Analysis of cellular senescence-related genes in calcified aortic valve disease and the potential therapeutic role of β-Carotene
PLoS ONE,
Journal Year:
2025,
Volume and Issue:
20(3), P. e0318574 - e0318574
Published: March 10, 2025
Objective
Calcific
aortic
valve
disease
(CAVD)
is
a
progressive,
age-related
degenerative
characterized
by
the
accumulation
of
calcium
deposits
in
valve.
We
aim
to
screen
key
genes
associated
with
cellular
senescence
(CS)
CAVD.
Methods
The
GSE12644
and
GSE51472
datasets
from
GEO
database
was
utilized
this
study,
differentially
expressed
(DEGs)
were
identified
using
“
limma
”
R
package.
CS-related
DEGs
(CS-DEGs)
determined
through
CellAge
database.
Gene
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
enrichment
analyses
performed
on
CS-DEGs.
A
protein–protein
interaction
(PPI)
network
constructed
STRING
cytoHubba
plug-in
Cytoscape
used
identify
hub
genes.
noncoding-RNA-mRNA
regulatory
established.
DSigDB
drugs
potentially
be
useful
for
treating
Results
total
16
CS-DEGs
identified.
These
primarily
collagen
metabolic
process,
catabolic
process
external
side
plasma
membrane.
10
as
regulators
CAVD:
LPAR1,
PTPN6,
CD28,
ID1,
MEIS2,
FGFR3,
KDR,
MMP7,
AR,
HIF1A.
Noncoding
RNA-mRNA
indicated
that
may
regulated
noncoding
RNAs.
β-Carotene,
naturally
occurring
carotenoid
antioxidant
properties,
potential
therapeutic
agents
interacting
MMP9,
CTSB.
Conclusion
This
study
provides
insights
into
pathways
related
CAVD
(MMP9,
CTSB)
highlights
role
β-Carotene
treatment
Language: Английский
Exploring Protein Functions of Gut Bacteriome and Mycobiome in Thai Infants Associated with Atopic Dermatitis Through Metaproteomic and Host Interaction Analysis
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(24), P. 13533 - 13533
Published: Dec. 18, 2024
Atopic
dermatitis
(AD),
a
prevalent
allergic
skin
condition
in
children,
has
been
closely
associated
with
imbalances
the
gut
microbiome.
To
investigate
these
microbial
alterations
and
their
functional
implications,
we
investigated
protein
expression,
functions
interactions
of
bacteriome
mycobiome
as
well
human
proteome
Thai
infants
AD
using
integrative
metaproteomic
host
interaction
analysis.
As
observed,
probiotic
species,
such
Lactobacillus
acidophilus
Bacteroides
salyersiae,
were
reduced
abundance
group
while
key
pathogenic
bacteria
fungi,
Streptococcus
constellatus
Penicillium
chrysogenum,
increased
abundance.
Additionally,
analysis
expressed
proteins
was
enriched
response
to
stress
DNA
repair
ribosome
biogenesis-related
processes
group,
potentially
reactive
oxygen
species
(ROS),
intestinal
inflammation,
fungal
growth
dysbiosis.
Further,
protein–protein
(PPIs)
network
incorporating
revealed
10
signature
related
immune
system
AD.
Our
findings
propose
between
microbes
infants.
our
knowledge,
this
study
serves
first
framework
for
monitoring
bacteriome–mycobiome–human
studies
other
diseases
Language: Английский