Meloidogyne enterolobiiMeMSP1 effector targets the glutathione‐S‐transferase phi GSTF family in Arabidopsis to manipulate host metabolism and promote nematode parasitism DOI
Yongpan Chen, Qian Liu, Xuqian Sun

et al.

New Phytologist, Journal Year: 2023, Volume and Issue: 240(6), P. 2468 - 2483

Published: Oct. 12, 2023

Meloidogyne enterolobii is an emerging root-knot nematode species that overcomes most of the resistance genes in crops. Nematode effector proteins secreted planta are key elements molecular dialogue parasitism. Here, we show MeMSP1 into giant cells and promotes M. Using co-immunoprecipitation bimolecular fluorescent complementation assays, identified glutathione-S-transferase phi GSTFs as host targets effector. This protein family plays important roles plant responses to abiotic biotic stresses. We demonstrate interacts with all Arabidopsis GSTF. Moreover, confirmed N-terminal region AtGSTF9 critical for its interaction, atgstf9 mutant lines more susceptible infection. Combined transcriptome metabolome analyses showed affects metabolic pathways thaliana, resulting accumulation amino acids, nucleic their metabolites, organic acids downregulation flavonoids. Our study has shed light on a novel mechanism metabolism, reducing production defence-related compounds while favouring metabolites beneficial nematode, thereby promoting

Language: Английский

Chromosome‐scale genome assembly‐assisted identification of Mi‐9 gene in Solanum arcanum accession LA2157 , conferring heat‐stable resistance to Meloidogyne incognita DOI Creative Commons
Lijun Jiang, Jian Ling, Jianlong Zhao

et al.

Plant Biotechnology Journal, Journal Year: 2023, Volume and Issue: 21(7), P. 1496 - 1509

Published: April 19, 2023

Summary Root‐knot nematodes (RKNs) are infamous plant pathogens in tomato production, causing considerable losses agriculture worldwide. Mi‐1 is the only commercially available RKN‐resistance gene; however, resistance inactivated when soil temperature over 28 °C. Mi‐9 wild ( Solanum arcanum LA2157) has stable to RKNs under high but not been cloned and applied. In this study, a chromosome‐scale genome assembly of S. LA2157 was constructed through Nanopore Hi‐C sequencing. Based on molecular markers comparative genomic analysis, localization region candidate genes cluster consisting seven nucleotide‐binding sites leucine‐rich repeat (NBS‐LRR) were located. Transcriptional expression profiles confirmed that five expressed root tissue. Moreover, virus‐induced gene silencing Sarc_034200 resulted increased susceptibility Meloidogyne incognita , genetic transformation susceptible pimpinellifolium conferred significant M. at 25 °C 30 showed hypersensitive responses nematode infection sites. This suggested gene. summary, we cloned, applied heat‐stable which great significance breeding for resistance.

Language: Английский

Citations

9
Unveiling the Diversity: Plant Parasitic Nematode Effectors and Their Plant Interaction Partners DOI Creative Commons

Sapinder Bali,

Cynthia Gleason

Molecular Plant-Microbe Interactions, Journal Year: 2023, Volume and Issue: 37(3), P. 179 - 189

Published: Oct. 23, 2023

Root-knot and cyst nematodes are two groups of plant parasitic that cause the majority crop losses in agriculture. As a result, these focus most nematode effector research. effectors defined as secreted molecules, typically proteins, with crucial roles parasitism. There likely hundreds molecules exuded through stylet into plant. The current research has shown can target variety host proteins have impacts include suppression immune responses manipulation hormone signaling. discovery localize to nucleus indicates directly modulate gene expression for cellular reprogramming during feeding site formation. In addition, peptide mimicry by some highlights sophisticated strategies employ manipulate processes. Here we describe on interactions between will provide insights molecular mechanisms underpinning plant-nematode interactions. By identifying pathways targeted root-knot effectors, scientists gain better understanding how establish sites subvert responses. Such information be invaluable future engineering nematode-resistant crops, ultimately fostering advancements agricultural practices protection. [Formula: see text] author(s) dedicated work public domain under Creative Commons CC0 "No Rights Reserved" license waiving all his or her rights worldwide copyright law, including related neighboring rights, extent allowed 2024.

Language: Английский

Citations

9
Revisiting the Critical Role of ROS and RNS in Plant Defense DOI
Krishna Kumar

Journal of Plant Growth Regulation, Journal Year: 2022, Volume and Issue: 42(10), P. 6202 - 6227

Published: Oct. 10, 2022

Language: Английский

Citations

14
Efficient control of root-knot nematodes by expressing Bt nematicidal proteins in root leucoplasts DOI
Yong Wang, Mengnan Wang, Yali Zhang

et al.

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(10), P. 1504 - 1519

Published: Aug. 14, 2024

Language: Английский

Citations

2
A root-knot nematode effector manipulates the rhizosphere microbiome for establishing parasitism relationship with hosts DOI Creative Commons
Rui Liu,

Mengfei Chen,

B. Liu

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: July 19, 2023

Introduction Root-knot nematode (RKN; Meloidogyne spp.) is one of the most infamous soilborne plant diseases, causing severe crop losses every year. Effector proteins secreted by RKNs play crucial roles during plant-nematode interaction. However, less known about whether RKN effector can impact rhizosphere microbial environment. Methods In this study, we investigated microbiome community MiMIF-2 (a immunity-modulating effector) transgenic Arabidopsis thaliana with or without infection using Illumina high-throughput sequencing analysis. Results and discussion The results showed that bacterial species richness index increased, while fungi decreased in M. incognita -infected A. plants. relative abundance genera such as Clitopilus, Komagataeibacter, Lactobacillus, Prevotella, Moritella, Vibrio, Escherichia-Shigella , Pseudomonas was reduced plants compared to wild type, but significantly increased after inoculation . Cluster Orthologous Genes (COG) function classification analysis revealed a decrease defense mechanisms, secondary metabolite biosynthesis, transport, catabolism-related functions lines type. These differences may be reason for susceptibility infection. Our provide new insight into their association community, host, pathogens, which will lead exploration innovative ideas future biological control RKNs.

Language: Английский

Citations

4
The root‐knot nematode effector MiEFF12 targets the host ER quality control system to suppress immune responses and allow parasitism DOI Creative Commons
Salomé Soulé,

Kaiwei Huang,

Karine Mulet

et al.

Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(7)

Published: July 1, 2024

Abstract Root‐knot nematodes (RKNs) are microscopic parasitic worms able to infest the roots of thousands plant species, causing massive crop yield losses worldwide. They evade plant's immune system and manipulate cell physiology metabolism transform a few root cells into giant cells, which serve as feeding sites for nematode. RKN parasitism is facilitated by secretion in planta effector molecules, mostly proteins that hijack host cellular processes. We describe here conserved RKN‐specific effector, 12 (EFF12), synthesized exclusively oesophageal glands nematode, we demonstrate its function parasitism. In plant, MiEFF12 localizes endoplasmic reticulum (ER). A combination RNA‐sequencing analysis immunity‐suppression bioassays revealed contribution modulation immunity. Yeast two‐hybrid, split luciferase co‐immunoprecipitation approaches identified an essential component ER quality control system, Solanum lycopersicum bap‐like (PBL), basic leucine zipper 60 (BZIP60) targets MiEFF12. Finally, silencing PBL genes Nicotiana benthamiana decreased susceptibility Meloidogyne incognita infection. Our results suggest EFF12 manipulates modify responses allow

Language: Английский

Citations

1
Genetic dissection of Meloidogyne incognita resistance genes based on VIGS functional analysis in Cucumis metuliferus DOI Creative Commons
Xiaoxiao Xie, Jian Ling, Junru Lu

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 15, 2024

The southern root-knot nematode, Meloidogyne incognita, is a highly serious plant parasitic nematode species that causes significant economic losses in various crops, including cucumber (Cucumis sativus L.). Currently, there are no commercial cultivars available with resistance to M. incognita cucumber. However, the African horned melon metuliferus Naud.), semi-wild relative of cucumber, has shown high incognita. In this study, we constructed an ultrahigh-density genetic linkage bin-map using low-coverage sequences from F

Language: Английский

Citations

1
A Meloidogyne incognita effector Minc03329 suppresses plant immunity and promotes parasitism DOI Creative Commons

Jing-jing ZHOU,

Xiao-ping ZHANG,

Rui Liu

et al.

Journal of Integrative Agriculture, Journal Year: 2022, Volume and Issue: 22(3), P. 799 - 811

Published: Aug. 28, 2022

Meloidogyne incognita is a devastating plant-parasitic nematode. Effectors play important roles during the stages of nematodes infection and parasitism, but their molecular functions remain largely unknown. In this study, we characterized new effector, Minc03329, which contains signal peptide for secretion C-type lectin domain. The yeast sequence trap experiments indicated that Minc03329 functional. situ hybridization showed was specifically expressed in subventral esophageal gland. Real-time qPCR confirmed expression level transcript significantly increased pre-parasitic parasitic second-stage juveniles (pre-J2s par-J2s). Tobacco rattle virus (TRV)-mediated gene silencing host plants reduced pathogenicity nematodes. On contrary, ectopic Arabidopsis thaliana plant susceptibility to Transient Nicotiana benthamiana leaves suppressed programmed cell death triggered by pro-apoptotic protein BAX. Moreover, transcriptome analysis Minc03329-transgenic wild type revealed many defense-related genes were down-regulated. Interestingly, some different involved formation nematode feeding sites. These results an effector M. incognita, suppressing defense response promoting pathogenicity.

Language: Английский

Citations

6
Effects of Silicon Nanoparticles on the Activity of Antioxidant Enzymes in Tomato Roots Invaded by Meloidogyne incognita (Kofoid et White, 1919) Chitwood, 1949 DOI

Ж. В. Удалова,

S. V. Zinovieva

Doklady Biochemistry and Biophysics, Journal Year: 2022, Volume and Issue: 506(1), P. 191 - 194

Published: Oct. 1, 2022

Language: Английский

Citations

5
Genome-Guided Reanalysis of Root-Knot Nematode Meloidogyne incognita Esophageal Gland Cell-Enriched Sequence Tag Libraries: A Resource for the Discovery of Novel Effectors DOI Creative Commons
Melissa G. Mitchum, Raquel O. Rocha, Guozhong Huang

et al.

PhytoFrontiers™, Journal Year: 2023, Volume and Issue: 3(2), P. 435 - 441

Published: Jan. 10, 2023

Language: Английский

Citations

2