Genomic analyses elucidate the causes and consequences of breakdown of distyly inLinum trigynum DOI Creative Commons
Juanita Gutiérrez‐Valencia, Panagiotis‐Ioannis Zervakis, Zoé Postel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 17, 2023

Abstract Distyly is an iconic floral polymorphism governed by a supergene, which promotes efficient pollen transfer and outcrossing through reciprocal differences in the position of sexual organs flowers, often coupled with heteromorphic self-incompatibility (SI). has evolved convergently multiple flowering plant lineages, but also broken down repeatedly, resulting homostylous, self-compatible populations elevated rates self-fertilization. Here, we aimed to study genetic causes genomic consequences shift homostyly Linum trigynum , closely related distylous tenue. Building on high-quality genome assembly, show that L. harbors region homologous dominant haplotype distyly supergene conferring long stamens short styles tenue suggesting loss first occurred short-styled individual. In contrast homostylous Primula Fagopyrum no fixed loss-of-function mutations coding sequences S- linked candidate genes. Instead, gene expression analyses controlled crosses suggest downregulating LtWDR-44 for male SI and/or anther height could underlie self-compatibility (SC) . Population 224 whole-genome further demonstrate highly self-fertilizing, exhibits significantly lower diversity genome-wide, experiencing relaxed purifying selection less frequent positive nonsynonymous relative Our shed light advance our understanding common evolutionary transition plants.

Language: Английский

The molecular basis of phenotypic evolution: beyond the usual suspects DOI
Rong‐Chien Lin,

Bianca T Ferreira,

Yao‐Wu Yuan

et al.

Trends in Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: May 1, 2024

Language: Английский

Citations

5
PfPIN5 promotes style elongation by regulating cell length in Primula forbesii Franch DOI Open Access
Ying Liu,

Weijia Si,

Sitong Fu

et al.

Annals of Botany, Journal Year: 2024, Volume and Issue: 133(3), P. 473 - 482

Published: Jan. 8, 2024

Abstract Background and Aims Style dimorphism is one of the polymorphic characteristics flowers in heterostylous plants, which have two types flowers: pin morph, with long styles shorter anthers, thrum short longer anthers. The formation dimorphic has received attention plant world. Previous studies showed that CYP734A50 Primula determined style length limited elongation brassinosteroid metabolic pathway was involved regulation length. However, it unknown whether there are other factors affecting Primula. Methods Differentially expressed genes highly morph were screened based on forbesii transcriptome data. Virus-induced gene silencing used to silence these genes, anatomical changes observed 20 days after injection. Key Results PfPIN5 styles. When silenced, shortened long-homostyle plants by shortening cells. Moreover, increased expression level significantly, increased. results indicated PfPIN5, an auxin efflux transporter gene, contributed P. forbesii. Conclusions implied might also be forbesii, providing a new for elucidating molecular mechanism

Language: Английский

Citations

4
A hemizygous supergene controls homomorphic and heteromorphic self-incompatibility systems in Oleaceae DOI
Pauline Raimondeau,

Sayam Ksouda,

William Marande

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(9), P. 1977 - 1986.e8

Published: April 15, 2024

Language: Английский

Citations

4
The Monochoria genome provides insights into the molecular mechanisms underlying floral heteranthery DOI
Jingshan Yang, Jinming Chen, Xiangyan He

et al.

Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Genomic studies inLinumshed light on the evolution of the distyly supergene and the molecular basis of convergent floral evolution DOI Creative Commons
Panagiotis‐Ioannis Zervakis, Zoé Postel, Aleksandra Losvik

et al.

Published: April 17, 2025

Summary Distyly, an example of convergent evolution, is governed by a supergene called the S- locus. Recent studies highlight similar genetic architectures independently evolved S -loci, but whether regulatory pathways underlie evolution distyly remains unclear. We examined supergenes and mechanisms underlying in Linum species that diverged ∼33 Mya. Using haplotype-resolved genomes population genomics, we identified characterized loci perenne (distylous) grandiflorum (style length dimorphic), compared them to tenue (distylous). then tested for conserved hormonal mechanism regulating style polymorphism . Hemizygosity short-styled individuals shared feature -locus supergene, though its size, gene content, repeat elements, extent recombination suppression vary greatly among species. Two candidate genes, TSS1 length) WDR-44 (anther height/pollen self-incompatibility) are at Consistent with brassinosteroid-dependent role , epibrassinolide treatment revealed conserved, morph-specific effect on length. locus architecture, key genes remain >30 Mya In combination findings from other systems, our results suggest brassinosteroid pathway frequently contributes polymorphism.

Language: Английский

Citations

0
Genetic Causes and Genomic Consequences of Breakdown of Distyly in Linum trigynum DOI Creative Commons
Juanita Gutiérrez‐Valencia, Panagiotis‐Ioannis Zervakis, Zoé Postel

et al.

Molecular Biology and Evolution, Journal Year: 2024, Volume and Issue: 41(5)

Published: May 1, 2024

Abstract Distyly is an iconic floral polymorphism governed by a supergene, which promotes efficient pollen transfer and outcrossing through reciprocal differences in the position of sexual organs flowers, often coupled with heteromorphic self-incompatibility. has evolved convergently multiple flowering plant lineages, but also broken down repeatedly, resulting homostylous, self-compatible populations elevated rates self-fertilization. Here, we aimed to study genetic causes genomic consequences shift homostyly Linum trigynum, closely related distylous tenue. Building on high-quality genome assembly, show that L. trigynum harbors region homologous dominant haplotype distyly supergene conferring long stamens short styles tenue, suggesting loss first occurred short-styled individual. In contrast homostylous Primula Fagopyrum, no fixed loss-of-function mutations coding sequences S-linked candidate genes. Instead, gene expression analyses controlled crosses suggest downregulating LtWDR-44 for male self-incompatibility and/or anther height could underlie self-compatibility trigynum. Population 224 whole-genome further demonstrate highly self-fertilizing, exhibits significantly lower diversity genome-wide, experiencing relaxed purifying selection less frequent positive nonsynonymous relative Our shed light advance our understanding common evolutionary transition plants.

Language: Английский

Citations

3
Converging on long and short: The genetics, molecular biology and evolution of heterostyly DOI Creative Commons
Lele Shang,

Karol Gad,

Michael Lenhard

et al.

Current Opinion in Plant Biology, Journal Year: 2025, Volume and Issue: 85, P. 102731 - 102731

Published: May 3, 2025

Heterostyly is a fascinating floral polymorphism that enhances outcrossing. In heterostylous species the flowers of two or three morphs differ in multiple traits, including reciprocal reproductive-organ placement and self-incompatibility. These traits are controlled by individual genes within an S-locus supergene, whose suppressed recombination ensures coordinated inheritance morph phenotypes. Recent breakthroughs about genetic molecular basis heterostyly have resulted from studies on many independently evolved instances include following: The hemizygous region comprising several taxa. systems, single gene plays dual roles regulating both female style length self-incompatibility type, often involving brassinosteroid signalling. S-loci through stepwise segmental duplication different lineages. frequent breakdown generally results mutations at leads to genomic selfing syndrome. discoveries suggest convergent genetically constrained evolution level.

Language: Английский

Citations

0
The Primula edelbergii S‐locus is an example of a jumping supergene DOI Creative Commons
Giacomo Potente, Narjes Yousefi, Barbara Keller

et al.

Molecular Ecology Resources, Journal Year: 2024, Volume and Issue: 24(6)

Published: June 30, 2024

Abstract Research on supergenes, non‐recombining genomic regions housing tightly linked genes that control complex phenotypes, has recently gained prominence in genomics. Heterostyly, a floral heteromorphism promoting outcrossing several angiosperm families, is controlled by the S ‐locus supergene. The been studied primarily closely related Primula species and, more recently, other groups independently evolved heterostyly. However, it remains unknown whether genetic architecture and composition of are maintained among share common origin heterostyly subsequently diverged across larger time scales. To address this research gap, we present chromosome‐scale genome assembly edelbergii , shares same with veris (whose characterized) but from 18 million years ago. Comparative analyses between these two allowed us to show, for first time, can ‘jump’ (i.e. translocate) chromosomes maintaining its function controlling Additionally, found four were conserved reshuffled within supergene, seemingly without affecting their expression, thus could not detect changes explaining lack self‐incompatibility P. . Furthermore, confirmed undergoing degeneration. Finally, investigated evolutionary history Ericales terms whole duplications transposable element accumulation. In summary, our work provides valuable resource comparative aimed at investigating genetics pivotal role supergenes shaping evolution phenotypes.

Language: Английский

Citations

2
Genomic evidence for supergene control of Darwin’s “complex marriage arrangement” – the tristylous floral polymorphism DOI Creative Commons
Haoran Xue, Yunchen Gong, Stephen Wright

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 11, 2024

Abstract Tristyly is a polymorphism characterized by three flower morphs with reciprocal stigma and anther heights controlled two epistatically interacting diallelic loci ( S M ), hypothesized to be supergenes. Chromosome-level genome assemblies of Eichhornia paniculata identified the S- M- loci. The -locus supergene consisting divergent alleles: -allele (2.51Mb) specific genes hemizygous in most S-morph plants s (596kb) five genes. Two genes, LAZY1-S HRGP-S, were specifically expressed styles stamens, respectively, making them tristyly candidate contained one gene (LAZY1-M), homologous LAZY1-S, that was present but absent from m -allele. Estimates allele ages are consistent prediction locus evolved before -locus. Re-use same family highlights potential role duplication evolution epistatic multilocus polymorphisms.

Language: Английский

Citations

1
Phosphoproteomic analysis of distylous Turnera subulata identifies pathways related to endoreduplication that correlate with reciprocal herkogamy DOI Creative Commons
Paige M. Henning, Benjamin B. Minkoff, Michael R. Sussman

et al.

American Journal of Botany, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

A multi-omic approach was used to explore proteins and networks hypothetically important for establishing filament dimorphisms in heterostylous Turnera subulata (Sm.) as an exploratory method identify genes future empirical research.

Language: Английский

Citations

1