Overexpression of Lol-miR11467 negatively affects osmotic resistance in Larix kaempferi 3 × L. gmelinii 9
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 10, 2025
Abstract
Background
Larch
(
Larix
gmelinii
(Rupr.)
Kuzen.)
is
an
important
timber
and
ecological
tree
species
in
northern
China.
Excellent
germplasm
resources
have
been
acquired
through
time-consuming
traditional
breeding.
Molecular
breeding
offers
a
promising
approach
to
shorten
the
cycle
achieve
genetic
improvements
more
efficiently.
MicroRNAs
(miRNAs)
are
non-coding,
single-stranded
small
RNAs
that
primarily
affect
plant
growth
stress
resistance,
including
drought
stress.
However,
study
of
miRNAs
larch
under
has
not
well
explored.
Results
In
this
study,
investigate
function
Lol-miR11467
PEG
osmotic
larch,
embryogenic
callus
tissue
Larix
kaempferi
3
×
L.
9
was
employed
as
experimental
material,
serving
explants
for
study.
Lol-miR11467was
transferred
into
using
Agrobacterium-mediated
method
determine
physiological
changes
survey
gene
expression
overexpressing
cell
lines.
The
results
showed
fresh
weight,
peroxidase
(POD),
soluble
protein
sugar
content
were
lower
than
wild-type,
while
malondialdehyde
(MDA)
increased
osmoticstress.
Transcriptome
analysis
genes
associated
with
phenylpropanoid
metabolism,
transcription
factors,
oxidoreductase,
hormone
signal
transduction,
glucose
metabolism
bioprotective
macromolecules
mainly
downregulated
Conclusions
Overall,
these
indicated
resistance
lines
reduced.
This
study'sfindings
might
provide
foundation
understanding
molecular
mechanisms
potentially
contributing
development
strategies
improving
resilience
environmental
stresses.
Language: Английский
Overexpression of Lol-miR11467 negatively affects osmotic resistance in Larix kaempferi 3 × L. gmelinii 9
BMC Plant Biology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: May 6, 2025
Language: Английский