Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 1, 2024

Arthrobotrys oligospora has been utilized as a model strain to study the interaction between fungi and nematodes owing its ability capture by developing specialized traps. A previous showed that high-osmolarity glycerol (Hog1) signaling regulates osmoregulation nematocidal activity of A. oligospora. However, function downstream transcription factors Hog1 in nematode-trapping (NT) remains unclear. This aimed investigate functions potential regulatory network AoMsn2, factor pathway The AoMsn2 was characterized using targeted gene deletion, phenotypic experiments, real-time quantitative PCR, RNA sequencing, untargeted metabolomics, yeast two-hybrid analysis. Loss Aomsn2 significantly enlarged swollen hyphae, with an increase septa significant decrease nuclei. In particular, spore yield, germination rate, traps, nematode predation efficiency were remarkably decreased mutants. Phenotypic transcriptomic analyses revealed is essential for fatty acid metabolism autophagic pathways. Additionally, metabolomic analysis identified important modulation secondary metabolites. Furtherly, we analyzed protein based on Kyoto Encyclopedia Genes Genomes map online website STRING. Finally, six putative proteins Y2H Our reveals plays crucial roles growth, conidiation, trap development, metabolism, well establishes broad basis understanding mechanisms morphogenesis environmental adaptation NT fungi.

Language: Английский

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Fus3 regulates asexual development and trap morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora

iScience, Journal Year: 2023, Volume and Issue: 26(8), P. 107404 - 107404

Published: Aug. 1, 2023

Mitogen-activated protein kinase (MAPK) Fus3 is an essential regulator of cell differentiation and virulence in fungal pathogens plants animals. However, the function regulatory mechanism MAPK signaling nematode-trapping (NT) fungi remain largely unknown. NT can specialize formation "traps", important indicator transition from a saprophytic to predatory lifestyle. Here, we characterized orthologous typical fungus Arthrobotrys oligospora using multi-phenotypic analysis multi-omics approaches. Our results showed that plays role asexual growth development, conidiation, stress response, DNA damage, autophagy, secondary metabolism. Importantly, indispensable hyphal fusion, trap morphogenesis, nematode predation. Moreover, constructed networks by means transcriptomic yeast two-hybrid techniques. This study provides insights into development pathogenicity fungi.

Language: Английский

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AoMedA has a complex regulatory relationship with AoBrlA, AoAbaA, and AoWetA in conidiation, trap formation, and secondary metabolism in the nematode-trapping fungus Arthrobotrys oligospora

Applied and Environmental Microbiology, Journal Year: 2023, Volume and Issue: 89(9)

Published: Sept. 1, 2023

The asexual sporulation of filamentous fungi is an important mechanism for their reproduction, survival, and pathogenicity. In Aspergillus several fungi, BrlA, AbaA, WetA are the key elements a central regulatory pathway controlling conidiation, MedA developmental modifier that regulates temporal expression genes; however, roles largely unknown in nematode-trapping (NT) fungi. Arthrobotrys oligospora representative NT fungus, which can capture nematodes by producing adhesive networks (traps). Here, we characterized function AoMedA three regulators (AoBrlA, AoAbaA, AoWetA) A. gene disruption, phenotypic comparison, multi-omics analyses, as these required conidiation play divergent mycelial development, trap formation, lipid droplet accumulation, vacuole assembly, secondary metabolism. A combined analysis traits transcriptome showed AoWetA involved regulation peroxisome, endocytosis, autophagy. Moreover, yeast one-hybrid AoBrlA regulate AoMedA, AoWetA, whereas AoAbaA AoWetA. Our results highlight AoBrlA, mycelia pathogenicity provide basis elucidating relationship between formation IMPORTANCE Conidiation most common reproductive mode many plays essential role fungal pathogens. Nematode-trapping special group owing to innate abilities digest traps (trapping devices). Sporulation growth reproduction conidia basic components biocontrol reagents diseases caused plant-parasitic nematodes. well-known fungus routinely used model probing interaction this study, functions four (AoMedA, were oligospora. complex was noted; pleiotropic multiple intracellular activities. study first revealed oligospora, contributed helped developing effective

Language: Английский

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The Hog1-Nmd5 signaling pathway regulates asexual development, lipid metabolism, stress response, trap morphogenesis, and secondary metabolism of Arthrobotrys oligospora

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 19, 2025

The high-osmolarity glycerol (HOG) signalling pathway, comprising Ste11/Ssk2/Ssk22 (MAPKKK), Pbs2 (MAPKK), and Hog1 (MAPK), is an important conserved pathway in fungi. However, the functions downstream regulatory factors of nematode-trapping (NT) fungi remain poorly understood. Here, three proteins (AoNmd5, AoPyp1, AoPtp) interacting with were screened a representative NT fungus Arthrobotrys oligospora using yeast screening library verified two-hybrid (Y2H) assay. function AoNmd5 was furtherly characterized by phenotypic comparison, staining technique, multi-omics analyses. essential for vegetative growth, conidial development, trap morphogenesis, nematode predation ability. In addition, played crucial roles endocytosis, lipid metabolism, reactive oxygen species, stress response, autophagy, other metabolic processes. Furthermore, we constructed interaction network based on transcriptomic analysis Y2H, revealing its significant role respiratory chain redox processes as well small GTPase Ran1, which mediates nucleocytoplasmic shuttling. These findings suggest that Hog1-Nmd5 has pleiotropic A. oligospora. This study deepens our understanding HOG importins

Language: Английский

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AoRab7A interacts with AoVps35 and AoVps41 to regulate vacuole assembly, trap formation, conidiation, and functions of proteasomes and ribosomes in Arthrobotrys oligospora

Microbiological Research, Journal Year: 2023, Volume and Issue: 280, P. 127573 - 127573

Published: Dec. 12, 2023

Language: Английский

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Cellular communication and fusion regulate cell fusion, trap morphogenesis, conidiation, and secondary metabolism in Arthrobotrys oligospora

Microbiological Research, Journal Year: 2023, Volume and Issue: 278, P. 127516 - 127516

Published: Oct. 12, 2023

Language: Английский

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Characterizing the Role of AosfgA and AofluG in Mycelial and Conidial Development in Arthrobotrys oligospora and Their Role in Secondary Metabolism

Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 615 - 615

Published: March 19, 2024

Arthrobotrys oligospora, a widespread nematode-trapping fungus which can produce conidia for asexual reproduction and form trapping devices (traps) to catch nematodes. However, little is known about the sporulation mechanism of A. oligospora. This research characterized functions regulatory roles upstream spore-producing genes, AosfgA AofluG, in Our analysis showed that AofluG interacted with each other. Meanwhile, gene was downregulated ΔAosfgA mutant strain, indicating positively regulates AofluG. Loss genes led shorter hyphae more septa, strain responded heat chemical stresses. Surprisingly, number nuclei increased mycelia but reduced ΔAofluG mutants. In addition, after nematode induction, volume vacuoles were remarkably strains. The abundance metabolites markedly decreased Collectively, play critical mycelial development, they are also involved vacuole assembly, stress response, secondary metabolism. study provides distinct insights into fungi.

Language: Английский

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AoMae1 Regulates Hyphal Fusion, Lipid Droplet Accumulation, Conidiation, and Trap Formation in Arthrobotrys oligospora

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(4), P. 496 - 496

Published: April 21, 2023

Malate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid (TCA) cycle and essential for energy balance, growth, tolerance to cold salt stresses plants. However, role of MDH filamentous fungi still largely unknown. In this study, we characterized an ortholog (AoMae1) representative nematode-trapping (NT) fungus Arthrobotrys oligospora via gene disruption, phenotypic analysis, nontargeted metabolomics. We found that loss Aomae1 led weakening activity ATP content, remarkable decrease conidia yield, considerable increase number traps mycelial loops. addition, absence also caused obvious reduction septa nuclei. particular, AoMae1 regulates hyphal fusion under low nutrient conditions but not nutrient-rich conditions, volumes sizes lipid droplets dynamically changed during trap formation nematode predation. involved regulation secondary metabolites such as arthrobotrisins. These results suggest has important fusion, sporulation, production, formation, pathogenicity A. oligospora. Our enhance understanding crucial enzymes TCA play development, NT fungi.

Language: Английский

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The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity of Ceratocystis fimbriata: Insights from Non-Targeted Metabolomics

Microorganisms, Journal Year: 2023, Volume and Issue: 11(11), P. 2666 - 2666

Published: Oct. 30, 2023

Ceratocystis fimbriata (C. fimbriata) is a notorious pathogenic fungus that causes sweet potato black rot disease. The APSES transcription factor Swi6 in fungi located downstream of the cell wall integrity (CWI)-mitogen-activated protein kinase (MAPK) signaling pathway and has been identified to be involved virulence several filamentous fungi. However, specific mechanisms by which regulates growth pathogenicity plant remain elusive. In this study, SWI6 deletion mutants complemented strains C. were generated. Deletion resulted aberrant patterns. Pathogenicity assays on storage roots revealed significant decrease mutant. Non-targeted metabolomic analysis using LC-MS total 692 potential differentially accumulated metabolites (PDAMs) ∆Cfswi6 mutant compared wild type, results KEGG enrichment demonstrated PDAMs within various metabolic pathways, including amino acid metabolism, lipid nucleotide GPI-anchored synthesis, ABC transporter metabolism. These pathways believed play crucial role mediating through regulation CWI. Firstly, gene led abnormal potentially exacerbating energy imbalance. Secondly, related biosynthesis implied compromised integrity. Lastly, disruption transport metabolism may hinder intracellular transmembrane transport. Importantly, study represents first investigation into regulatory from perspective. findings provide novel insights fimbriata, highlighting its as target for controlling pathogen.

Language: Английский

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An Oxidoreductase-like Protein is Required for Verticillium dahliae Infection and Participates in the Metabolism of Host Plant Defensive Compounds

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(9), P. 4669 - 4678

Published: Feb. 21, 2024

Verticillium dahliae, a notorious phytopathogenic fungus, is responsible for vascular wilt diseases in numerous crops. Uncovering the molecular mechanisms underlying pathogenicity crucial controlling V. dahliae. Herein, we characterized putative oxidoreductase-like protein (VdOrlp) from dahliae that contains functional signal peptide. While expression of VdOrlp was low artificial media, it significantly increased during host infection. Deletion had minimal effects on growth and development but severely impaired its pathogenicity. Metabolomic analysis revealed significant changes organic heterocyclic compounds phenylpropane cotton plants infected with ΔVdOrlp V991. Furthermore, induced by lignin, deletion affected metabolism lignin phenolic acids. In conclusion, our results demonstrated plays an important role plant phenylpropyl required fungal

Language: Английский

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