Genomic surveillance of SARS-CoV-2 variants in Guangzhou, China, from April 2023 to March 2024

Infection Genetics and Evolution, Journal Year: 2025, Volume and Issue: unknown, P. 105747 - 105747

Published: April 1, 2025

After the relaxation of stringent control measures, nationwide large-scale SARS-CoV-2 surveillance was gradually phased out post-2023, transitioning to focused monitoring Influenza-like Illness (ILI) through sentinel hospitals and laboratory networks. Nationally, respiratory pathogens performed via random sampling, resulting in a lack microbial results Guangzhou China. A crucial area scientific inquiry is whether current cases are attributable emergence novel variant. Throat swab samples were obtained from 1478 outpatients 337 hospitalized patients with fever (temperature ≥ 38 °C) cough or sore throat detect SARS-CoV-2. The positive subjected viral whole-genome sequencing phylogenetic analysis. Respiratory pathogen multiplex PCR tests on stratified samples. detected 517 (28.48 %) patients. There higher rates infection among women, older those who hospitalized. total 299 high-quality sequences obtained, including 12 clades 71 pango lineages. advantageous evolved over three peak periods infection, BA.5 (April 2023) XBB (June July then JN.1 (February 2024). 590 distinct amino acid mutations identified across sequences. highest prevalence observed for spike protein mutations, more than 50 % epidemic peaks detected. Epidemiological profiles interactions between other exhibit considerable variation different seasons, tendency toward suppression within each. Surveillance by Eighth People's Hospital provides snapshot Guangzhou, which consistent national offers important data understanding spread southern

Language: Английский

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Biological factors associated with long COVID and comparative analysis of SARS-CoV-2 spike protein variants: a retrospective study in Thailand

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17898 - e17898

Published: Aug. 19, 2024

Post-acute COVID-19 syndrome (long COVID) refers to the persistence of symptoms or exceptional following recovery. Even without conferring fatality, it represents a significant global public health burden. Despite many reports on long COVID, prevalence and data associated biological factors remain unclear limited. This research aimed determine COVID during two distinct epidemic periods in Thailand, due Delta Omicron variants SARS-CoV-2, investigate with COVID. In addition, spike protein amino acid sequences were compared frequency mutations their potential implications.

Language: Английский

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Mapping disparities in viral infection rates using highly multiplexed serology

mSphere, Journal Year: 2024, Volume and Issue: 9(9)

Published: Aug. 20, 2024

ABSTRACT Despite advancements in medical interventions, the disease burden caused by viral pathogens remains large and highly diverse. This includes wide range of signs symptoms associated with active replication as well a variety clinical sequelae infection. Moreover, there is growing evidence supporting existence sex- ethnicity-based health disparities linked to infections their diseases. several well-documented infection rates, our current understanding virus-associated incomplete. knowledge gap can be attributed, part, limitations most commonly used detection methodologies, which lack breadth needed characterize exposures across entire virome. Additionally, virus-related are dynamic often differ considerably through space time. In this study, we utilize PepSeq, an approach for multiplexed serology, broadly assess individual’s history exposures, demonstrate effectiveness detecting pilot study 400 adults aged 30–60 Phoenix, AZ. Using human virome PepSeq library, observed expected seroprevalence rates common viruses detected both previously undocumented differences inferred between male/female Hispanic/non-Hispanic White individuals. IMPORTANCE Our population-level virus because high diversity human-infecting limited sensitivity traditional approaches events. Here, potential modern, antibody methods greatly increase subpopulations (e.g., different sexes or ethnic groups). The use antibodies biomarkers allows us detect past over extended period, serology (PepSeq) measure responses against hundreds efficient cost-effective manner.

Language: Английский

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Dynamic expedition of leading mutations in SARS-CoV-2 spike glycoproteins

Computational and Structural Biotechnology Journal, Journal Year: 2024, Volume and Issue: 23, P. 2407 - 2417

Published: May 24, 2024

The continuous evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused the recent pandemic, has generated countless new variants with varying fitness. Mutations spike glycoprotein play a particularly vital role in shaping its evolutionary trajectory, as they have capability to alter infectivity and antigenicity. We present time-resolved statistical method, Dynamic Expedition Leading (deLemus), analyze dynamics SARS-CoV-2 glycoprotein. proposed

Language: Английский

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