NAR Genomics and Bioinformatics,
Journal Year:
2024,
Volume and Issue:
6(4)
Published: Sept. 28, 2024
Abstract
There
is
a
growing
interest
in
generating
bimodal,
single-cell
RNA
sequencing
(RNA-seq)
data
for
studying
biological
pathways.
These
are
predominantly
utilized
understanding
phenotypic
trajectories
using
velocities;
however,
the
shape
information
encoded
two-dimensional
resolution
of
such
not
yet
exploited.
In
this
paper,
we
present
an
elliptical
parametrization
RNA-seq
data,
from
which
derived
statistics
that
reveal
four
different
modalities.
modalities
can
be
interpreted
as
manifestations
changes
rates
splicing,
transcription
or
degradation.
We
performed
our
analysis
on
cell
cycle
and
colorectal
cancer
dataset.
both
datasets,
found
genes
picked
up
by
differential
gene
expression
(DGEA),
consequently
unnoticed,
visibly
delineate
phenotypes.
This
indicates
that,
addition
to
DGEA,
searching
exhibit
discovered
could
aid
recovering
set
phenotypes
apart.
For
communities
biomarkers
cellular
phenotyping,
bimodal
broaden
search
space
genes,
furthermore,
allow
incorporating
processing
into
regulatory
analyses.
Molecular Medicine,
Journal Year:
2025,
Volume and Issue:
31(1)
Published: March 12, 2025
Abstract
Chemoresistance
continues
to
pose
a
significant
challenge
in
managing
colorectal
cancer
(CRC),
resulting
unfavorable
outcomes
for
patients.
Recent
findings
indicate
that
ferroptosis,
an
innovative
type
of
regulated
cell
death,
might
influence
chemoresistance.
In
this
research,
we
explored
how
WW
domain-binding
protein
1
(WBP1)
affects
mitochondrial
function,
growth,
and
chemoresistance
CRC
cells.
By
employing
both
genetic
pharmacological
methods,
found
WBP1
is
essential
maintaining
respiration
depletion
impaired
leading
reduced
proliferation
increased
ferroptosis.
Exogenous
mitochondria
from
wild-type
cells
restored
proliferation,
suppressed
ferroptosis
WBP1-deficient
cells,
indicating
function
acts
downstream
WBP1.
Importantly,
demonstrated
targeting
or
its
mediated
sensitized
chemoresistant
5-fluorouracil
oxaliplatin
by
inducing
Furthermore,
analyzed
transcriptome
data
patients,
which
indicated
expression
correlated
with
poor
patients
receiving
chemotherapy,
thus
highlighting
the
clinical
significance
our
observations.
Collectively,
results
pinpoint
as
modulator
imply
may
represent
viable
approach
tackling
These
insights
offer
deeper
understanding
molecular
pathways
underlying
guide
development
new
treatment
options.
Investigación Clínica,
Journal Year:
2025,
Volume and Issue:
66(1), P. 39 - 48
Published: March 7, 2025
This
research
aimed
to
clarify
the
clinical
impact
of
early
enteral
nu-trition
(EN)
on
postoperative
pain,
gastrointestinal
function
and
nutritional
status
colorectal
cancer
(CRC)
patients.
Eighty
rectal
patients
undergoing
sur-gery
in
our
hospital
from
October
2021
2023
were
selected
as
subjects
divided
into
an
experimental
group
(EG)
a
control
(CG)
using
random
number
table
method,
with
40
cases
each.
Both
groups
received
conventional
nursing,
including
preoperative,
intraoperative,
nursing.
The
CG
traditional
preoperative
routine
diet
EN
support.
EG
five-day
support
parenteral
nutrition
based
diet.
analgesic
effect
indicators,
pain
scores,
recovery
indica-tors,
adverse
reactions
indicators
both
measure-ment
comparison.
Average
additional
amount
flurbiprofen
axetil
decreased
relative
those
(p<0.05).
At
six
h
12
after
surgery,
VAS
scores
lower
than
during
same
period;
at
24
48
no
statistical
significance
was
shown
be-tween
(p>0.05).
bowel
sound
time,
first
defecating
exhaust
getting-out-of-bed
time
inferior
incidence
reduced
that
Before
surgery
one
day
statistically
significant
differences
total
protein
(TP)
serum
albumin
(ALB)
levels
between
(p>0.05);
three
days
seven
TP
ALB
exhibited
elevation
period
In
conclusion,
can
improve
not
only
but
also
mitigate
facilitate
high
safety,
which
is
worthy
further
promotion.
Research and Practical Medicine Journal,
Journal Year:
2025,
Volume and Issue:
12(1), P. 26 - 39
Published: March 1, 2025
Purpose
of
the
study
.
To
examine
influence
specific
factors
on
apoptosis
process
in
mitochondria
cells
located
various
regions
colon,
with
a
focus
patients
both
sexes.
Patients
and
methods
The
included
results
obtained
from
132
T2–3N0M0
colon
cancer,
comprising
52
women
80
men.
Mitochondria
were
isolated
human
intestinal
tumor
tissue
using
differential
centrifugation.
concentration
cytochrome
C
(ng/mg
protein),
AIF
(pg/mg
Bcl-2
calcium
(mM/mg
protein)
determined.
Results
In
men,
level
rectal
left
was
reduced
relative
to
corresponding
along
resection
line,
while
levels
Bcl-2,
C,
were,
contrary,
creased.
At
same
time,
right
did
not
differ
statistically
significantly
values
increased
by
2.0
3.1
times.
observed
be
factor
1.5
women,
comparison
indicator
line
(
p
<
0.05).
Cytochrome
1.5,
2.9,
2.1
times,
respectively.
half
1.6
times
0.05),
2.7
1.7
higher,
respectively,
than
indicators
Conclusion
A
comprehensive
analysis
studied
suggests
that
is
suppressed
all
large
intestine
men
women.
Conversely,
processes
respiration
mitochondrial
energy
production
appear
enhanced.
Genes,
Journal Year:
2025,
Volume and Issue:
16(3), P. 268 - 268
Published: Feb. 25, 2025
The
mitochondria–telomere
axis
is
recognized
as
an
important
factor
in
the
processes
of
metabolism,
aging
and
oncogenesis.
MicroRNAs
(miRNAs)
play
essential
function
this
complex
interaction,
having
impact
on
aspects
such
cellular
homeostasis,
oxidative
responses
apoptosis.
In
recent
years,
miRNAs
have
been
found
to
be
crucial
for
telomeric
stability,
well
mitochondrial
behavior,
factors
that
influence
cell
proliferation
viability.
Furthermore,
(mitomiRs)
are
associated
with
gene
expression
activity
cGAS/STING
pathway
activity,
linking
DNA
recognition
immune
system
responses.
Hence,
maintain
a
link
biogenesis,
metabolic
changes
cancer
organelles.
This
review
focuses
roles
variety
progression
their
potential
application
biomarkers
or
therapeutic
agents.
BMC Cancer,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: March 14, 2025
Mitochondrial-encoded
circular
RNAs
(mecciRNAs)
are
a
newly
discovered
class
of
mitochondrial-encoded
non-coding
(mt-ncRNAs)
that
play
important
biological
roles
in
the
cell.
This
study
aimed
to
examine
expression
profile
SCAR/mc-COX2
(has_circ_0089762)
colorectal
cancer
(CRC)
and
its
relationship
with
clinicopathological
variables.
Furthermore,
better
understand
SCAR/mc-COX2's
functional
role
CRC,
we
constructed
competing
endogenous
RNA
(ceRNA)
network.
Quantitative
real-time
PCR
(qRT-PCR)
was
employed
analyze
levels
40
pairs
CRC
samples,
consisting
tumor
samples
adjacent
non-tumoral
from
patients.
The
ceRNA
regulatory
network
using
online
bioinformatics
tools.
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathway
analysis
Gene
Ontology
(GO)
enrichment
were
conducted
Enrichr
database.
results
demonstrated
significant
decrease
tissues
compared
(p-value<0.05).
In
another
finding,
observed
between
pathological
T
staging
status
(p-value=0.02).
Additionally,
Receiver
Operating
Characteristic
(ROC)
curve
revealed
had
an
area
under
(AUC)
0.77,
80%
sensitivity
75%
specificity.
Finally,
including
SCAR/mc-COX2,
5
miRNA,
9
mRNAs
found.
These
findings
suggest
may
act
as
suppressor
dysregulation
could
crucial
pathophysiology
this
cancer.
association
robust
diagnostic
performance
(AUC
=
80%,
specificity
75%)
highlight
potential
novel
biomarker
for
detection
prognosis.
Further
studies
required
elucidate
precise
tumorigenesis
clinical
applicability.
