PROX1 Controls Cellular Patterning, Innervation and Differentiation in the Mouse Organ of Corti DOI Creative Commons

Shubham Kale,

Dimitri Traenkner,

Ellison J. Goodrich

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract The organ of Corti is divided into functional compartments responsible for hearing or cochlear amplification. A medial compartment containing inner hair cells innervated by Type I spiral ganglion neurons and a lateral outer II neurons. Supporting also differ, with pillar Deiters’ developing specialized cellular structures to support cell electromotility. We bred Corti-restricted Prox1 conditional knockout mice study development because PROX1 the first transcription factor expressed strictly in supporting cells. In absence , numbers increased without corresponding changes cells, they appear incompletely differentiated based on morphological criteria. Outer number was not impacted but innervation disrupted many afferent turning incorrectly towards apex. RNAseq revealed no gene expression that could account phenotype. Therefore, we propose promotes differentiation organization has secondary effects required specification.

Language: Английский

Not a hair out of place: Polarizing and orienting sensory hair cells DOI
Katie S. Kindt, Basile Tarchini

Current topics in developmental biology/Current Topics in Developmental Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
PROX1 Controls Cellular Patterning, Innervation and Differentiation in the Mouse Organ of Corti DOI Creative Commons

Shubham Kale,

Dimitri Traenkner,

Ellison J. Goodrich

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract The organ of Corti is divided into functional compartments responsible for hearing or cochlear amplification. A medial compartment containing inner hair cells innervated by Type I spiral ganglion neurons and a lateral outer II neurons. Supporting also differ, with pillar Deiters’ developing specialized cellular structures to support cell electromotility. We bred Corti-restricted Prox1 conditional knockout mice study development because PROX1 the first transcription factor expressed strictly in supporting cells. In absence , numbers increased without corresponding changes cells, they appear incompletely differentiated based on morphological criteria. Outer number was not impacted but innervation disrupted many afferent turning incorrectly towards apex. RNAseq revealed no gene expression that could account phenotype. Therefore, we propose promotes differentiation organization has secondary effects required specification.

Language: Английский

Citations

0