Fourier Raman light field microscopy based on surface-enhanced Raman scattering DOI
Jingfan Wang, Ming Chen, Xing Zhao

et al.

Optics Letters, Journal Year: 2024, Volume and Issue: 49(16), P. 4693 - 4693

Published: Aug. 5, 2024

Raman scattering, as a vibrational spectrum that carries material information, has no photobleaching enables long-duration imaging. spectra have very narrow emission peaks, and multiplex imaging can be achieved by using different scattering peak signals. These advantages make widely used in biology, cytology, medicine, which wider range of application scenarios. However, obtaining three-dimensional (3D) image requires scanning for tens minutes to several hours at present. Therefore, fast non-scanning 3D method is greatly needed. In this article, we propose Fourier light field microscopy based on surface-enhanced (sers-FRLFM). Using flower-like gap-enhanced nanoparticles (F-GERNs) enhance signals, Fourier-configured microscope (LFM) capable recording complete four-dimensional information single frame, facilitating the reconstruction without generating artifacts native object plan. Moreover, F-GERNs mark specific locations potential become new tracing achieve This great real-time cells, microorganisms, tissues with lateral resolution 2.40 µm an axial 4.02 µm.

Language: Английский

Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy DOI Creative Commons
Nicolas Verrier, Matthieu Debailleul, Olivier Haeberlé

et al.

Sensors, Journal Year: 2024, Volume and Issue: 24(5), P. 1594 - 1594

Published: Feb. 29, 2024

Optical microscopy techniques are among the most used methods in biomedical sample characterization. In their more advanced realization, optical microscopes demonstrate resolution down to nanometric scale. These rely on use of fluorescent labeling order break diffraction limit. However, molecules’ phototoxicity or photobleaching is not always compatible with investigated samples. To overcome this limitation, quantitative phase imaging have been proposed. Among these, holographic has demonstrated its ability image living microscopic samples without staining. for a 3D assessment samples, tomographic acquisitions needed. Tomographic Diffraction Microscopy (TDM) combines reconstructions. Relying synthetic aperture process, TDM allows measurements complex refractive index sample. Since initial proposition by Emil Wolf 1969, concept found lot applications and become one hot topics imaging. This review focuses recent achievements development. Current trends perspectives technique also discussed.

Language: Английский

Citations

5
Fourier Raman light field microscopy based on surface-enhanced Raman scattering DOI
Jingfan Wang, Ming Chen, Xing Zhao

et al.

Optics Letters, Journal Year: 2024, Volume and Issue: 49(16), P. 4693 - 4693

Published: Aug. 5, 2024

Raman scattering, as a vibrational spectrum that carries material information, has no photobleaching enables long-duration imaging. spectra have very narrow emission peaks, and multiplex imaging can be achieved by using different scattering peak signals. These advantages make widely used in biology, cytology, medicine, which wider range of application scenarios. However, obtaining three-dimensional (3D) image requires scanning for tens minutes to several hours at present. Therefore, fast non-scanning 3D method is greatly needed. In this article, we propose Fourier light field microscopy based on surface-enhanced (sers-FRLFM). Using flower-like gap-enhanced nanoparticles (F-GERNs) enhance signals, Fourier-configured microscope (LFM) capable recording complete four-dimensional information single frame, facilitating the reconstruction without generating artifacts native object plan. Moreover, F-GERNs mark specific locations potential become new tracing achieve This great real-time cells, microorganisms, tissues with lateral resolution 2.40 µm an axial 4.02 µm.

Language: Английский

Citations

1