Optimizing Filament-Based TCP Scaffold Design for Osteoconduction and Bone Augmentation: Insights from In Vivo Rabbit Models DOI Creative Commons
Julien Guerrero, Ekaterina Maevskaia, Chafik Ghayor

et al.

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(7), P. 174 - 174

Published: June 25, 2024

Additive manufacturing has emerged as a transformative tool in biomedical engineering, offering precise control over scaffold design for bone tissue engineering and regenerative medicine. While much attention been focused on optimizing pore-based architectures, filament-based microarchitectures remain relatively understudied, despite the fact that majority of 3D-printers generate structures. Here, we investigated influence filament characteristics regeneration outcomes using lithography-based additive approach. Three distinct scaffolds (Fil050, Fil083, Fil125) identical macroporosity transparency, crafted from tri-calcium phosphate (TCP) with varying thicknesses distance, were evaluated rabbit model augmentation non-critical calvarial defect. Additionally, two types differing directionality (Fil FilG) compared to elucidate optimal parameters. Distance ingrowth percentage regenerated area within measured by histomorphometric analysis. Our findings reveal filaments 0.50 mm most effective scaffold, demonstrating superior bony larger size (i.e., 0.83 1.25 scaffolds). Optimized can overcome reduced performance filaments. This study advances our understanding microarchitecture’s role holds significant implications clinical practice, paving way development highly tailored, patient-specific substitutes enhanced efficacy.

Language: Английский

Role of Bioceramics for Bone Regeneration; History, Mechanisms and Future Perspective DOI Open Access
Md Amit Hasan Tanvir, Abdul Khaleque,

Ga-Hyun Kim

et al.

Published: April 4, 2024

Osteoporosis is a bone condition where bones become weaker, leading to fractures, especially in older adults and postmenopausal women. Bioceramics for regeneration have indeed emerged as promising solution conditions like osteoporosis. Choosing the right bioceramic depends on how quickly it dissolves, strong is, whether body will react it. Studies show that bioceramics can help grow back by activating (bone morphogenetic protein) BMP, (mitogen-activated protein kinase) MAPK, Wingless/integrated (Wnt)/β-catenin pathways when combined with stem cells, drugs, supports. However, some problems not being flexible enough prone breaking, well difficulties growing cells finding supports different types. While there has been progress improving healing, we need keep looking new ideas from other areas of medicine them. This review aims add expanding field scientific research offering detailed look at growth factors contribute regeneration. Ultimately, this knowledge creating types improve regrowth, providing treatment options people diseases.

Language: Английский

Citations

9
Polycaprolactone for Hard Tissue Regeneration: Scaffold Design and In Vivo Implications DOI Creative Commons
Fernanda Ramírez-Ruiz, Israel Núñez-Tapia, Cristina Piña‐Barba

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(1), P. 46 - 46

Published: Jan. 8, 2025

In the last thirty years, tissue engineering (TI) has emerged as an alternative method to regenerate tissues and organs restore their function by implanting specific lineage cells, growth factors, or biomolecules functionalizing a matrix scaffold. Recently, several pathologies have led bone loss damage, such malformations, resorption associated with benign malignant tumors, periodontal disease, traumas, others in which discontinuity integrity is observed. Bone characterized different stiffness, mechanical traction, compression resistance of compartments, can influence susceptibility injury destruction. For this reason, research into repairing defects began years ago find scaffold improve regeneration. Different techniques be used manufacture 3D scaffolds for regeneration based on optimizing reproducible controlled hierarchical porous structure like extracellular bone. Additionally, synthesized facilitate inclusion mesenchymal stem cells factors that osteogenesis, recruiting new neighborhood generate optimal environment review, current state-of-the-art manufacturing use polycaprolactone (PCL) biomaterial will described reporting relevant studies focusing processing techniques, from traditional—i.e., freeze casting, thermally induced phase separation, gas foaming, solvent particle leaching—to more recent approaches, additive (i.e., printing/bioprinting, electrofluid dynamics/electrospinning), well integrated techniques. As technique, work aims offer comprehensive overview benefits/limitations PCL-based order establish relationship between composition, namely integration other phases’ structural properties pore morphology properties) vivo response.

Language: Английский

Citations

1
Comparative Analysis of Gene Expression in Periodontal Ligament Stem Cells Exposed to Biodentine and Bio-C Repair: Implications for Cementogenesis—An In Vitro Study DOI Creative Commons
Mahmoud M. Bakr, Mahmoud Al Ankily, Mohammed Meer

et al.

Oral, Journal Year: 2025, Volume and Issue: 5(1), P. 19 - 19

Published: March 13, 2025

Background/Objectives: Bioactive materials are gaining increased popularity as of choice for pulpal regeneration. A similar trend is emerging with root repair materials; however, there a significant gap in the literature about cementogenic ability bioceramic on periodontal ligament cells. The aim present study was to investigate effect (Biodentine and Bio-C Repair) cementogenesis potential stem cells (PDLSCs). Methods: PDLSCs were isolated using enzymatic digestion approach from sound extracted teeth. Material extracts prepared rubber discs immersed fresh growth medium 24 h at 37 °C. Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) used detect mRNA expression levels markers cementum protein 1 (CEMP1), Cementum attachment (CAP), pathway transforming factor β1(TGF-β1), bone morphogenic 2 (BMP2), inflammatory marker IL-6. Results: Both showed significantly higher gene expressions when compared control groups. Repair Biodentine, except TGF-β1 expression, where both exhibited results. Conclusions: demonstrated Biodentine under tested conditions. Further vivo studies deemed necessary translate findings this into clinical practice.

Language: Английский

Citations

0
Three-Dimensional Bioprinting for Intervertebral Disc Regeneration DOI Creative Commons
Md Amit Hasan Tanvir,

Md Abdul Khaleque,

Junhee Lee

et al.

Journal of Functional Biomaterials, Journal Year: 2025, Volume and Issue: 16(3), P. 105 - 105

Published: March 14, 2025

The rising demand for organ transplants and the need precise tissue models have positioned in vitro biomanufacturing of tissues organs as a pivotal area regenerative treatment. Considerable development has been achieved growing tissue-engineered intervertebral disc (IVD) scaffolds, designed to meet stringent mechanical biological compatibility criteria. Among cutting-edge approaches, 3D bioprinting stands out due its unparalleled capacity organize biomaterials, bioactive molecules, living cells with high precision. Despite these advancements, polymer-based scaffolds still encounter limitations replicating extracellular matrix (ECM)-like environment, which is fundamental optimal cellular activities. To overcome challenges, integrating polymers hydrogels recommended promising solution. This combination enables advancement porous that nurture cell adhesion, proliferation, well differentiation. Additionally, bioinks derived from decellularized (dECM) exhibited potential biologically relevant microenvironments, enhancing viability, differentiation, motility. Hydrogels, whether natural sources involving collagen alginate or synthesized chemically, are highly valued their ECM-like properties superior biocompatibility. review will explore recent advancements techniques technologies IVD regeneration. Emphasis be placed on identifying research gaps proposing strategies bridge them, goal accelerating translation IVDs into clinical applications.

Language: Английский

Citations

0
Innovative Approaches in Bone Tissue Engineering: Strategies for Cancer Treatment and Recovery DOI Open Access

Samiksha Khobragade,

Manish Deshmukh,

Ujwal Vyas

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 3937 - 3937

Published: April 22, 2025

Cancer has rapidly emerged as a leading global cause of premature mortality, with significant economic implications projected to reach USD 25.2 trillion from 2020 2050. Among the various types cancer, primary bone cancers, though uncommon, are see nearly 4000 new cases diagnosed in United States 2024. The complexity treating cancer arises its rarity, diversity, and challenges associated surgical interventions, metastatic spread, post-operative complications. Advancements tissue engineering (BTE) have introduced innovative therapeutic approaches promote regeneration address tumor recurrence. This interdisciplinary field integrates biomaterials, scaffolds, gene therapy, utilizing technologies such 3D bioprinting create custom scaffolds that facilitate cellular activities essential for regeneration. Recent developments biodegradable, bioactive materials aim enhance biocompatibility effectiveness while nanotechnology presents promising avenues targeted drug delivery improved outcomes. review outlines current landscape BTE, highlighting scaffold fabrication techniques, advantages incorporating stem cell therapies, future directions, including integration artificial intelligence design personalized medicine orthopedic oncology. work underscores necessity ongoing research innovation, aiming improve strategies specifically designed unique posed by sarcomas cancers.

Language: Английский

Citations

0
Biological Behavior of Bioactive Glasses SinGlass (45S5) and SinGlass High (F18) in the Repair of Critical Bone Defects DOI Creative Commons
Dayane Maria Braz Nogueira, Marcelie Priscila de Oliveira Rosso, Paulo Sérgio da Silva Santos

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(1), P. 112 - 112

Published: Jan. 13, 2025

This study evaluated the osteogenic potential of bioactive glasses SinGlass (45S5) and High (F18) in regenerating critical bone defects rat calvaria. Both biomaterials promoted new formation around particles, with group exhibiting a higher rate maturation. Histomorphological birefringence analyses revealed better organization newly formed biomaterial-treated groups, immunohistochemistry indicated expression markers such as osteocalcin, immunostaining for morphogenetic protein 2 (BMP 2), 4 4). Microtomography computadorized (Micro-CT) centripetal both greater integration particles into surrounding tissue. The superior performance was attributed to its potassium magnesium content, which enhance osteoconductivity. After 42 days, showed highest percentage formation, line previous studies. Although our results are promising, limited follow-up period use single animal model highlight need further research validate clinical applicability. appears be viable alternative autografts repair, improve tissue accelerate recovery.

Language: Английский

Citations

0
Effectiveness of the Association of Fibrin Scaffolds, Nanohydroxyapatite, and Photobiomodulation with Simultaneous Low-Level Red and Infrared Lasers in Bone Repair DOI Open Access

Jéssica de Oliveira Rossi,

Emilie Maria Cabral Araujo,

Maria Eduarda Côrtes Camargo

et al.

Materials, Journal Year: 2024, Volume and Issue: 17(17), P. 4351 - 4351

Published: Sept. 3, 2024

Biomaterials and biopharmaceuticals for correcting large bone defects are a potential area of translational science. A new bioproduct, purified from snake venom fibrinogen buffalo blood, aroused interest in the repair venous ulcers. Expanding uses, it has also been used to form biocomplexes combination with grafts, associated physical therapies or alone. The aim this preclinical study was evaluate low-level laser photobiomodulation (PBM) critical calvaria rats filled nanohydroxyapatite (NH) heterologous fibrin biopolymer (HFB). Sixty animals were used, divided into six groups (n = 10 each): G1 (NH); G2 (HFB); G3 (NH + HFB); G4 PBM); G5 (HFB G6 HFB PBM). PBM simultaneously red (R) infrared (IR) light emission, applied intraoperatively twice week, until end experiment at 42 days. Microtomography, formation can be seen initially margins defect, more evident G5. Microscopically, demonstrated immature disorganized trabeculation 14 days, remnants grafting materials. At percentage formed higher all groups, especially (HFB, 45.4 ± 3.82), collagen fibers degree maturation yellowish-green color birefringence analysis Picrosirius-red. Therefore, is concluded that showed greater effectiveness process presents future clinical studies.

Language: Английский

Citations

2
Significance of Necroptosis in Cartilage Degeneration DOI Creative Commons

Md Abdul Khaleque,

Jae‐Hoon Kim, Md Amit Hasan Tanvir

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1192 - 1192

Published: Sept. 21, 2024

Cartilage, a critical tissue for joint function, often degenerates due to osteoarthritis (OA), rheumatoid arthritis (RA), and trauma. Recent research underscores necroptosis, regulated form of necrosis, as key player in cartilage degradation. Unlike apoptosis, necroptosis triggers robust inflammatory responses, exacerbating damage. Key mediators such receptor-interacting serine/threonine-protein kinase-1 (RIPK1), kinase-3(RIPK3), mixed lineage kinase domain-like (MLKL) are pivotal this process. Studies reveal contributes significantly OA RA pathophysiology, where elevated RIPK3 associated proteins drive Targeting necroptotic pathways shows promise; inhibitors like Necrostatin-1 (Nec-1), GSK’872, Necrosulfonamide (NSA) reduce cell death, offering potential therapeutic avenues. Additionally, autophagy’s role mitigating necroptosis-induced damage highlights the need comprehensive strategies addressing multiple pathways. Despite these insights, further is essential fully understand necroptosis’ mechanisms develop effective treatments. This review synthesizes current knowledge on degeneration, aiming inform novel approaches OA, RA,

Language: Английский

Citations

2
Optimizing Filament-Based TCP Scaffold Design for Osteoconduction and Bone Augmentation: Insights from In Vivo Rabbit Models DOI Creative Commons
Julien Guerrero, Ekaterina Maevskaia, Chafik Ghayor

et al.

Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(7), P. 174 - 174

Published: June 25, 2024

Additive manufacturing has emerged as a transformative tool in biomedical engineering, offering precise control over scaffold design for bone tissue engineering and regenerative medicine. While much attention been focused on optimizing pore-based architectures, filament-based microarchitectures remain relatively understudied, despite the fact that majority of 3D-printers generate structures. Here, we investigated influence filament characteristics regeneration outcomes using lithography-based additive approach. Three distinct scaffolds (Fil050, Fil083, Fil125) identical macroporosity transparency, crafted from tri-calcium phosphate (TCP) with varying thicknesses distance, were evaluated rabbit model augmentation non-critical calvarial defect. Additionally, two types differing directionality (Fil FilG) compared to elucidate optimal parameters. Distance ingrowth percentage regenerated area within measured by histomorphometric analysis. Our findings reveal filaments 0.50 mm most effective scaffold, demonstrating superior bony larger size (i.e., 0.83 1.25 scaffolds). Optimized can overcome reduced performance filaments. This study advances our understanding microarchitecture’s role holds significant implications clinical practice, paving way development highly tailored, patient-specific substitutes enhanced efficacy.

Language: Английский

Citations

0