Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії, Journal Year: 2024, Volume and Issue: 24(4), P. 212 - 218
Published: Dec. 26, 2024
The study aimed to investigate the effect of transcription factor modulators on nitric oxide (NO) system parameters in blood rats under lipopolysaccharide (LPS)-induced systemic inflammatory response (SIR). experiment was conducted 42 male Wistar weighing 180–220 g, divided into 6 groups (7 animals per group): Group 1 included intact (Control I); 2 involved rats, which underwent LPS-induced (SIR) modeling II). In remaining groups, were administered SIR modeling: 3 received anticancer drug bortezomib (used treat multiple myeloma and mantle cell lymphoma), an NF-κB inhibitor (via proteasome suppression); 4 agent SR 11302 (investigated as a potential treatment for lung cancer), AP-1; 5 dimethyl fumarate, specific activator Nrf2–ARE signaling pathway; with quercetin, flavonoid that acts Nrf2 pathway activator. results showed significantly increased total NOS iNOS activity while reducing cNOS arginase serum. This indicates development nitrosative stress impaired L-arginine metabolism rats. use inhibitors (bortezomib) AP-1 (SR 11302), well activators (dimethyl fumarate quercetin) reduced partially normalized activity, demonstrating their anti-inflammatory metabolic effects. most effective agents correcting 11302, near-intact levels restored functional cNOS. Most (bortezomib, highlighting role metabolism. However, 11302) further decreased compared group, suggesting possible inhibition compensatory enzyme mechanisms exacerbation imbalance. findings confirm key roles NF-κB, AP-1, pathways regulating stress, offering promising pharmacological targets disturbances.
Language: Английский