Free Radical Research,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 13
Published: Dec. 8, 2024
Urban
aerosol
particulate
matter
(UPM)
is
widespread
in
the
environment,
and
its
concentration
continues
to
increase.
Several
recent
studies
have
reported
that
UPM
results
premature
cellular
senescence,
but
few
investigated
molecular
basis
of
UPM-induced
senescence
retinal
pigment
epithelial
(RPE)
cells.
In
this
study,
we
primarily
evaluated
protective
function
nuclear
factor
erythroid
2-related
2
(Nrf2)
human
RPE
ARPE-19
The
findings
indicated
exposure
substantially
induced
cells,
as
observed
by
increased
β-galactosidase
activity,
expression
levels
senescence-associated
marker
proteins,
phenotypes.
Such
associated
with
mitochondrial
oxidative
stress-mediated
phosphatidylinositol
3'-kinase/Akt/Nrf2
downregulation.
Sulforaphane-mediated
Nrf2
activation
upregulation
phosphorylated
suppressed
decrease
target
antioxidant
gene,
NAD(P)H
quinone
oxidoreductase
1,
under
UPM,
which
notably
prevented
cells
from
senescence.
By
contrast,
knockdown
exacerbated
promoted
stress.
Collectively,
our
demonstrate
regulatory
role
suggest
a
potential
target.
Pharmaceuticals,
Journal Year:
2024,
Volume and Issue:
17(11), P. 1493 - 1493
Published: Nov. 7, 2024
Ischemic
stroke
is
a
leading
cause
of
disability
and
mortality
worldwide,
with
current
therapies
limited
in
addressing
its
complex
pathophysiological
mechanisms,
such
as
inflammation,
oxidative
stress,
apoptosis,
impaired
autophagy.
Glycyrrhizic
acid
(GA),
bioactive
compound
from
licorice
(
Free Radical Biology and Medicine,
Journal Year:
2024,
Volume and Issue:
224, P. 335 - 345
Published: Aug. 30, 2024
Gasotransmitter-mediated
cysteine
post-translational
modifications,
including
S-nitrosylation
(SNO)
and
S-persulfidation
(SSH),
play
crucial
roles
interact
in
various
biological
processes.
However,
there
has
been
a
delay
appreciating
the
interactional
rules
between
SNO
SSH.
Here,
all
human
S-nitrosylated
S-persulfidated
proteomic
data
were
curated,
comprehensive
analyses
from
multiple
perspectives,
sequence,
structure,
function,
exact
protein
impacts
(e.g.,
up-/down-regulation),
performed.
Although
these
two
modifications
collectively
regulated
wide
array
of
proteins
to
jointly
maintain
redox
homeostasis,
they
also
exhibited
intriguing
differences.
First,
tended
be
more
accessible
functionally
clustered
pathways
associated
with
cell
damage
repair
other
such
as
phosphorylation
ubiquitination.
Second,
SSH
preferentially
targeted
cysteines
disulfide
bonds
modulated
tissue
development
immune-related
pathways.
Finally,
regardless
whether
occupied
same
position
given
protein,
their
combined
effect
suppressive
when
acting
synergistically;
otherwise,
likely
inhibited
while
activated
target
protein.
Indeed,
side-by-side
comparison
shed
light
on
globally
reciprocal
effects
provided
reference
for
further
research
gasotransmitter-mediated
effects.
Free Radical Research,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 13
Published: Dec. 8, 2024
Urban
aerosol
particulate
matter
(UPM)
is
widespread
in
the
environment,
and
its
concentration
continues
to
increase.
Several
recent
studies
have
reported
that
UPM
results
premature
cellular
senescence,
but
few
investigated
molecular
basis
of
UPM-induced
senescence
retinal
pigment
epithelial
(RPE)
cells.
In
this
study,
we
primarily
evaluated
protective
function
nuclear
factor
erythroid
2-related
2
(Nrf2)
human
RPE
ARPE-19
The
findings
indicated
exposure
substantially
induced
cells,
as
observed
by
increased
β-galactosidase
activity,
expression
levels
senescence-associated
marker
proteins,
phenotypes.
Such
associated
with
mitochondrial
oxidative
stress-mediated
phosphatidylinositol
3'-kinase/Akt/Nrf2
downregulation.
Sulforaphane-mediated
Nrf2
activation
upregulation
phosphorylated
suppressed
decrease
target
antioxidant
gene,
NAD(P)H
quinone
oxidoreductase
1,
under
UPM,
which
notably
prevented
cells
from
senescence.
By
contrast,
knockdown
exacerbated
promoted
stress.
Collectively,
our
demonstrate
regulatory
role
suggest
a
potential
target.
