Seaweeds-derived proteins and peptides: preparation, virtual screening, health-promoting effects, and industry applications

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 28

Published: Jan. 15, 2025

Seaweed, a promising source of nutritional proteins, including protein hydrolysates, bioactive peptides, phycobiliproteins, and lectins with multi-biological activities. Seaweeds-derived proteins peptides have attracted increasing interest for their potential applications in dietary supplements, functional foods, pharmaceuticals industries. This work aims to comprehensively review the preparation methods virtual screening strategies seaweed-derived peptides. Additionally, it elucidates diverse biological activities, mechanisms action, industrial applications. Enzymatic hydrolysis appears as most effective method preparing from seaweeds. Computational has also proven be valuable strategy assessing nature offer numerous health benefits, alleviation oxidative stress, anti-diabetic, anti-hypertensive, anti-inflammatory, anti-obesity, anti-cancer, anti-microbial Studies indicate that hydrolysates derived seaweeds low molecular weight aromatic and/or hydrophobic amino acids are particularly significant contributing these bio-activities. Furthermore, seaweeds-derived hold great promise owing broad spectrum bio-functional effects. They can used active ingredients food products or disease prevention treatment, preservatives, potentially fewer side

Language: Английский

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Harnessing the power of bee venom for therapeutic and regenerative medical applications: an updated review

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: July 18, 2024

Honeybees have been helpful insects since ancient centuries, and this benefit is not limited to being a honey producer only. After the bee stings person, pain, swelling occur in place, due effects of venom (BV). This poison total sense word because it has many benefits, its composition rich proteins, peptides, enzymes, other types molecules low concentrations that show promise treatment numerous diseases conditions. BV also demonstrated positive against various cancers, antimicrobial activity, wound healing versus human immunodeficiency virus (HIV). Even though topical therapy used varying degrees among countries, localized or itching are common side may some patients. review provides an in-depth analysis complex chemical BV, highlighting diverse range bioactive compounds their therapeutic applications, which extend beyond well-known anti-inflammatory pain-relieving effects, showcasing versatility modern medicine. A specific search strategy was followed across databases; Web sciences, Scopus, Medline, Google Scholar including vitro vivo clinical studies.to outline overview composition, methods use, preparation requirements, Individual consumption contraindications. Furthermore, addresses safety concerns emerging approaches, such as use nanoparticles, mitigate adverse demonstrating balanced holistic perspective. Importantly, incorporates historical context traditional uses, well unique focus on veterinary setting apart from previous works providing valuable resource for researchers practitioners field.

Language: Английский

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Food-derived bioactive peptides: The gateway to reach the full potential of food proteins for human health

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: 157, P. 104896 - 104896

Published: Jan. 31, 2025

Language: Английский

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Anticancer and Antioxidant Effects of Bioactive Peptides from Black Soldier Fly Larvae (Hermetia illucens)

Nutrients, Journal Year: 2025, Volume and Issue: 17(4), P. 645 - 645

Published: Feb. 11, 2025

Background: Protein hydrolysates from insects are recognized for their biological activities. Black soldier fly larvae (BSFL) have drawn attention due to antioxidant protein hydrolysates. However, research on bioactive peptides derived these hydrolysates, particularly cancer chemopreventive potential, remains limited. This study aims evaluate the antioxidant, anti-inflammatory, antimutagenic, and anticancer activities of BSFL-derived explore molecular mechanisms. Methods: Alkali-soluble BSFL (ASBP) was extracted hydrolyzed using Alcalase bromelain under optimized conditions. Antioxidant activity assessed via FRAP, ABTS, DPPH assays. The hydrolysate with highest fractionated into weight (MW) groups (>30, 10, <3 kDa). bioactivity evaluated through anti-inflammatory (nitric oxide production in RAW 264.7 cells), antimutagenic (Ames test), (CCK-8 assay HCT 116, COLO205, Cw-2, Caco-2 cells) Mechanistic insights were obtained microarray Western blot analyses. Peptides identified by LC-MS/MS. Results: ASBP-Alcalase (ASBP-AH) showed optimal at 3% (w/w) 4 h. ASBP-AH 30 (MW > kDa) fraction exhibited capacity. In contrast, ASBP-AH3 < 3 significant effects, reduced nitric production, decreased COLO205 cell viability. Treatment its LC50 dose modulated SKP2/p21/cyclin D1 pathways. Mostly composed hydrophobic charged amino acids. Conclusions: exhibit potential as multifunctional agents chemoprevention. vivo studies required clinical applications.

Language: Английский

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Functional characterization of enzyme-hydrolysed soy and whey protein isolates: A comparative approach

Food Chemistry Advances, Journal Year: 2024, Volume and Issue: 5, P. 100745 - 100745

Published: June 6, 2024

In the present study, soy protein and whey isolates were hydrolysed using food grade endoenzymes (papain pancreatin) for different time periods (10, 30, 60, 90, 120 minutes) at 35°C. Several functional properties examined various of hydrolysis. Papain both better than pancreatin through out hydrolysis period. The degree papain isolate was 1.12 after min During hydrolysis, particle size decreased initially followed by slight increase. higher isolate. Zeta potential during isolates. zeta inversely proportional to SDS-PAGE shown that, had a greater number peptide bands Emulsifying activity increased with time. Emulsion stability enzyme addition From this study it evident enzymatic defatted enhances its properties. Hence, can be used as cost-effective alternative isolate, vegan patients.

Language: Английский

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Bioactive peptides from food science to pharmaceutical industries: their mechanism of action, potential role in cancer treatment and available resources

Heliyon, Journal Year: 2024, Volume and Issue: 10(23), P. e40563 - e40563

Published: Nov. 22, 2024

Language: Английский

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Bioactive peptides with potential anticancer properties from various food protein sources: status of recent research, production technologies, and developments

Critical Reviews in Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 22

Published: Jan. 5, 2025

Recently, bioactive peptides, from natural resources, have attracted remarkable attention as nutraceutical treasures and the health benefits of their consumption extensively been studied. Therapies based on peptides recognized an innovative promising alternative method for dangerous diseases such cancer. Indeed, there has enormous interest in nutraceuticals bioactive-based chemopreventive molecules a potential opportunity to manage chronic diseases, including cancer at different stages, rather than traditionally used therapies. The relative safety efficacy these targeting only tumor cells without affecting normal make them attractive alternatives existing pharmaceuticals treatment, management, prevention cancer, being able act physiological modulators metabolism during intestinal digestion. Novel derived food sources can be beneficial anticancer provide basis pharmaceutical development food-derived peptides. Bioactive generated through protein hydrolysis methods purified using advanced chromatographic techniques. Moreover, establishing peptides' mechanism action management. Most research is carried out cell lines with very limited investigated animal models or human clinical models. In this context, review article comprehensively discusses peptides': production, isolation, therapeutic strategies, action, application therapy.

Language: Английский

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Anticarcinogenic cationic peptides derived from tryptic hydrolysis of β-lactoglobulin

Frontiers in Molecular Biosciences, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 7, 2025

This study investigated the tryptic hydrolysis of β-lactoglobulin (BLG) for 30, 60, 90, and 120 min at 1/200 E/S (enzyme/substrate ratio, w/w) to prepare potentially anticarcinogenic peptides. The properties hydrolysates were characterized, including degree hydrolysis, free amino acids, SDS-PAGE, FTIR, antioxidant activity employing DPPH-assay, β-carotene/linoleic acid, FRAP assay. BLG hydrolysate produced after 60 recorded highest activity, LCMS analysis revealed 162 peptides molecular masses ranging from 800 5671Da, most them are hydrophobic nature. Within low-MW peptide group (24 peptides), there nine basic (HB) seven acidic (HA), representing 38% 29%, respectively. HB may be responsible considerable biological hydrolysate. With dominant character supporting carcinogenic this in vitro anticancer against Mcf-7, Caco-2, A-549 human cancer cell lines proliferation by MTT assay IC50% 42.8, 76.92, 45.93 μg/mL, Treating each line with 24 h increased apoptosis enhancing expression caspase-9 5.66, 7.97, 3.28 folds over untreated control inhibited angiogenesis reducing VEGFR-2 about 56, 76, 70%, respectively, indicating strong antiangiogenic actions on cells. serve as a natural agent. results demonstrated that have direct effects chemical composition characteristics influence these activities. generally effective three studied (Mcf-7, A-549). effectiveness was assessed measuring using evaluating their impact through inhibition activity. Future studies focus isolating prominent individual varying treatment conditions.

Language: Английский

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Molecular Chimera in Cancer Drug Discovery: Beyond Antibody Therapy, Designing Grafted Stable Peptides Targeting Cancer

International Journal of Peptide Research and Therapeutics, Journal Year: 2025, Volume and Issue: 31(3)

Published: Feb. 17, 2025

Abstract Background Several cancer therapies are being developed, and given the variability of different types, goal these is to remove invasive tumor from body, kill cells, or else retard growth. These include chemotherapeutic agents targeted therapy using small molecules antibodies. However, antibodies can generate an immune response upon repeated administration, producing could be expensive. Purpose The purpose this review describe therapeutic approaches utilized for therapy, current approaches, their limitations. As a novel strategy combat cancer, designing new stable peptide scaffolds such as cyclotides sunflower trypsin inhibitors (SFTI) described. Results Stable peptides that target proteins used agents. Here, we utilization amalgamation plant-based with biological epitopes in called “Molecular Chimeras” grafted strategy. cyclic bind receptors modulate protein-protein interactions they high affinity selectivity. Grafted also possess better serum stability owing head-to-tail cyclization other structural modifications. Conclusion outweigh biologicals terms manufacturing process. Peptides peptidomimetics agents, provide alternatives molecule drugs.

Language: Английский

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Potential health benefits of lactoferrin and derived peptides – how to qualify as a medical device?

Critical Reviews in Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 25

Published: Feb. 18, 2025

ABSTRACTsLactoferrin (LF) is a glycoprotein, member of the transferrin family, and present in variety secretory fluids, including milk, saliva, tears, mucosal secretions. Iron binding, immunological regulation, antibacterial action, intestinal nutrition absorption are only few its important biological roles. Although much research has been done on human lactoferrin (hLF), LF derived from different animals equally essential for physiology health. Depending intended application mechanism goods containing peptide derivatives may be classified as medical devices under FDA rules or EU Directives. For regulations, product categorized device if it primarily provides antimicrobial health advantages. However, LFs not considered when used food addition supplement without particular medicinal claims. Safety efficacy data examined regulatory approval this category to guarantee appropriate usage usefulness clinical settings. When utilized various applications, wound healing, gastrointestinal problems, immune system stimulation, complex nature potential advantages their would consistent with categorization class II device. The role several species (especially cameloids) discussed paper products activities where satisfies requirements

Language: Английский

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