Identification of a Plasma Exosomal lncRNA‐ and circRNA‐Based ceRNA Regulatory Network in Patients With Lung Adenocarcinoma DOI Creative Commons
Wangyu Zhu, Huafeng Zhang, L.J. Tang

et al.

The Clinical Respiratory Journal, Journal Year: 2024, Volume and Issue: 18(10)

Published: Oct. 1, 2024

ABSTRACT Background Exosomes have been established to be enriched with various long noncoding RNAs (lncRNAs) and circular (circRNAs) that exert biological effects. However, the lncRNA‐ circRNA‐mediated coexpression competing endogenous RNA (ceRNA) regulatory network in exosomes derived from plasma of patients lung adenocarcinoma (LUAD) remains elusive. Methods Results This study enrolled nine three healthy individuals, differential expression messenger (mRNAs), lncRNAs, circRNAs was detected using microarray analysis, while microRNAs (miRNAs) were through sequencing. Additionally, bioinformatics algorithms applied evaluate lncRNA–miRNA–mRNAs/circRNA–miRNA–mRNA network. Differentially expressed cicRNAs identified via quantitative reverse transcription polymerase chain reaction (RT‐qPCR). A total 1016 1396 circRNAs, 45 miRNAs, 699 mRNAs differentially LUAD compared controls. Among them, 881 lncRNAs upregulated 135 downregulated, 916 480 miRNAs none 591 108 downregulated ( p ≤ 0.05, fold change ≥ 2). Gene Ontology (GO) analysis Kyoto Encyclopedia Genes Genomes (KEGG) pathway revealed functions RNAs. Meanwhile, networks displayed relationship between dysregulated Finally, RT‐qPCR validated circ‐0033861, circ‐0043273, circ‐0011959 exosome controls = 0.0327, 0.0002, 0.0437, respectively). Conclusion proposed a newly discovered ncRNA–miRNA–mRNA/circRNA–miRNA–mRNA ceRNA circulating up‐regulated LUAD, offering valuable insights for exploring potential function exosomal identifying biomarkers LUAD.

Language: Английский

Identification of a Plasma Exosomal lncRNA‐ and circRNA‐Based ceRNA Regulatory Network in Patients With Lung Adenocarcinoma DOI Creative Commons
Wangyu Zhu, Huafeng Zhang, L.J. Tang

et al.

The Clinical Respiratory Journal, Journal Year: 2024, Volume and Issue: 18(10)

Published: Oct. 1, 2024

ABSTRACT Background Exosomes have been established to be enriched with various long noncoding RNAs (lncRNAs) and circular (circRNAs) that exert biological effects. However, the lncRNA‐ circRNA‐mediated coexpression competing endogenous RNA (ceRNA) regulatory network in exosomes derived from plasma of patients lung adenocarcinoma (LUAD) remains elusive. Methods Results This study enrolled nine three healthy individuals, differential expression messenger (mRNAs), lncRNAs, circRNAs was detected using microarray analysis, while microRNAs (miRNAs) were through sequencing. Additionally, bioinformatics algorithms applied evaluate lncRNA–miRNA–mRNAs/circRNA–miRNA–mRNA network. Differentially expressed cicRNAs identified via quantitative reverse transcription polymerase chain reaction (RT‐qPCR). A total 1016 1396 circRNAs, 45 miRNAs, 699 mRNAs differentially LUAD compared controls. Among them, 881 lncRNAs upregulated 135 downregulated, 916 480 miRNAs none 591 108 downregulated ( p ≤ 0.05, fold change ≥ 2). Gene Ontology (GO) analysis Kyoto Encyclopedia Genes Genomes (KEGG) pathway revealed functions RNAs. Meanwhile, networks displayed relationship between dysregulated Finally, RT‐qPCR validated circ‐0033861, circ‐0043273, circ‐0011959 exosome controls = 0.0327, 0.0002, 0.0437, respectively). Conclusion proposed a newly discovered ncRNA–miRNA–mRNA/circRNA–miRNA–mRNA ceRNA circulating up‐regulated LUAD, offering valuable insights for exploring potential function exosomal identifying biomarkers LUAD.

Language: Английский

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