Establishing VIGS and CRISPR/Cas9 techniques to verify RsPDS function in radish DOI Creative Commons
Jiali Ying, Yan Wang,

Liang Xu

et al.

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: 23(5), P. 1557 - 1567

Published: March 22, 2024

Virus-induced gene silencing (VIGS) and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) systems are effective technologies for rapid accurate function verification in modern plant biotechnology. However, the investigation of editing radish remains limited. In this study, a bleaching phenotype was generated through knockdown RsPDS using tobacco rattle virus (TRV)- turnip yellow mosaic (TYMV)-mediated vectors. The TYMV-mediated efficiency higher than TRV-based VIGS system radish. expression level significantly inhibited 'NAU-067' leaves. rootless seedlings were infected with Agrobacterium rhizogenes 2300GN-Ubi-RsPDS-Cas9 vector two target sequences. Nine adventitious roots blue GUS staining, four these edited at sequence 1 as indicated by Sanger sequencing. Furthermore, albino lines A. tumefaciens-mediated transformation cotyledons. Five base substitutions three deletions occurred 2 Line 1, insertions 2. This study shows that CRISPR/Cas9 techniques can be employed to precisely verify biological functions genes radish, which will facilitate genetic improvement vital horticultural traits breeding programs.

Language: Английский

CRISPR technology: A decade of genome editing is only the beginning DOI
Joy Y. Wang, Jennifer A. Doudna

Science, Journal Year: 2023, Volume and Issue: 379(6629)

Published: Jan. 19, 2023

The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome editing, coupled with advances in computing and imaging capabilities, has initiated a new era which genetic diseases individual disease susceptibilities are both predictable actionable. Likewise, genes responsible for plant traits can be identified altered quickly, transforming the pace agricultural research breeding. In this Review, we discuss current state CRISPR-mediated manipulation human cells, animals, plants along relevant successes challenges present roadmap future technology.

Language: Английский

Citations

608
Gene therapy for CNS disorders: modalities, delivery and translational challenges DOI
Jingjing Gao,

Swetharajan Gunasekar,

Ziting Xia

et al.

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(8), P. 553 - 572

Published: June 19, 2024

Language: Английский

Citations

21
Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops DOI Open Access
Miguel Ángel Villalobos-López, Analilia Arroyo-Becerra,

Anareli Quintero-Jiménez

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(19), P. 12053 - 12053

Published: Oct. 10, 2022

The major challenges that agriculture is facing in the twenty-first century are increasing droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate change. However, most crops not tolerant climatic environments. aim near future, a world with hunger an population, breed and/or engineer tolerate abiotic stress higher yield. Some crop varieties display certain degree of tolerance, which has been exploited by plant breeders develop thrive under conditions. Moreover, long list genes involved tolerance have identified characterized molecular techniques overexpressed individually transformation experiments. Nevertheless, phenotypes polygenetic traits, current genomic tools dissecting exploit their use accelerating genetic introgression using markers or site-directed mutagenesis such as CRISPR-Cas9. In this review, we describe mechanisms sense adverse conditions examine discuss classic new select improve crops.

Language: Английский

Citations

50
Multiplex gene editing reveals cucumber MILDEW RESISTANCE LOCUS O family roles in powdery mildew resistance DOI
Mingru Ma, Li Yang, HU Zhen-zhu

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(2), P. 1069 - 1088

Published: Feb. 8, 2024

Abstract Powdery mildew (PM) is one of the most widespread and prevalent diseases that affects a wide range crops. In cucumber (Cucumis sativus L.), previous forward genetic studies have identified MILDEW RESISTANCE LOCUS O 8 (CsMLO8) as necessary but alone insufficient for PM resistance (PMR) suggested involvement other members CsMLO family. However, function family in remains largely unknown. Here, we developed highly efficient multiplex gene editing system to generate series Csmlo mutants from all 13 members. Systematic analysis these revealed growth effects on development PMR. Importantly, obtained Csmlo1/8/11 triple mutant with complete PM. Transcriptome proteome PM-resistant kinesin-like calmodulin-binding protein (KCBP)-interacting Ca2+-binding (CsKIC), calmodulin-like 28 (CsCML28), Ca2+-dependent kinase 11 (CsCPK11)-mediated calcium signaling pathway involved CsMLO8 interacted directly CsKIC, simultaneous silencing both genes resulted phenotype resembled CsKIC alone. Silencing CsCML28 CsCPK11 increased susceptibility PM, whereas overexpressing through transformation enhanced cucumber's PMR, demonstrating their positive regulatory roles Given importance PMR cucurbit crops, this research provides unprecedented insights into proteins encoded by well plant defense response pathogen.

Language: Английский

Citations

10
Emerging applications of gene editing technologies for the development of climate-resilient crops DOI Creative Commons
R. L. Chavhan,

Siddhant Gahininath Jaybhaye,

V. R. Hinge

et al.

Frontiers in Genome Editing, Journal Year: 2025, Volume and Issue: 7

Published: March 10, 2025

Climate change threatens global crop yield and food security due to rising temperatures, erratic rainfall, increased abiotic stresses like drought, heat, salinity. Gene editing technologies, including CRISPR/Cas9, base editors, prime offer precise tools for enhancing resilience. This review explores the mechanisms of these technologies their applications in developing climate-resilient crops address future challenges. While CRISPR/enables targeted modifications plant DNA, editors allow direct conversion without inducing double-stranded breaks, enable insertions, deletions, substitutions. By understanding manipulating key regulator genes involved stress responses, such as DREB, HSP, SOS, ERECTA, HsfA1, NHX; tolerance can be enhanced against salt stress. improve traits related root development, water use efficiency, response pathways, heat shock response, photosynthesis, membrane stability, ion homeostasis, osmotic adjustment, oxidative response. Advancements gene integration with genomics, phenomics, artificial intelligence (AI)/machine learning (ML) hold great promise. However, challenges off-target effects, delivery methods, regulatory barriers must addressed. highlights potential develop crops, contributing sustainable agriculture.

Language: Английский

Citations

1
Evolutionary dynamics and adaptive benefits of deleterious mutations in crop gene pools DOI Creative Commons

Sangam L. Dwivedi,

J. S. Heslop‐Harrison, Charles Spillane

et al.

Trends in Plant Science, Journal Year: 2023, Volume and Issue: 28(6), P. 685 - 697

Published: Feb. 8, 2023

Mutations with deleterious consequences in nature may be conditionally crop plants. That is, while some genetic variants reduce fitness under wild conditions and subject to purifying selection, they can positive selection domesticates. Such alleles plant breeding targets, particularly for complex traits. The difficulty of distinguishing favorable from unfavorable reduces the power trait variation heterosis attributable alleles. Here, we review roles mutations discuss how used as a new avenue improvement emerging genomic tools, including HapMaps pangenome analysis, aiding identification, removal, or exploitation mutations.

Language: Английский

Citations

22
Breeding rice for yield improvement through CRISPR/Cas9 genome editing method: current technologies and examples DOI
Rengasamy Balakrishnan, Mrinalini Manna,

Nargis Begum Thajuddin

et al.

Physiology and Molecular Biology of Plants, Journal Year: 2024, Volume and Issue: 30(2), P. 185 - 198

Published: Feb. 1, 2024

Language: Английский

Citations

8
The genetic orchestra of salicylic acid in plant resilience to climate change induced abiotic stress: critical review DOI Creative Commons

Mohamed Elsisi,

Moaz Elshiekh,

Nourine Sabry

et al.

Stress Biology, Journal Year: 2024, Volume and Issue: 4(1)

Published: June 17, 2024

Abstract Climate change, driven by human activities and natural processes, has led to critical alterations in varying patterns during cropping seasons is a vital threat global food security. The climate change impose several abiotic stresses on crop production systems. These include extreme temperatures, drought, salinity, which expose agricultural fields more vulnerable conditions lead substantial yield quality losses. Plant hormones, especially salicylic acid (SA), crucial roles for plant resiliency under unfavorable environments. This review explores the genetics molecular mechanisms underlying SA's role mitigating stress-induced damage plants. It also SA biosynthesis pathways, highlights regulation of their products stresses. Various possible modes action are discussed, along with unraveling genetic genes involved responses stress conditions. Additionally, this investigates pathways through exerts its protective effects, such as redox signaling, cross-talks other mitogen-activated protein kinase pathways. Moreover, discusses potentials using engineering approaches, CRISPR technology, deciphering enhancing resilience related comprehensive analysis bridges gap between response stressors. Overall goal highlight significance safeguarding plants offering insights hormone sustainable agriculture challenging environmental

Language: Английский

Citations

7
Africanizing genome editing for food sustainability DOI Creative Commons
Steven Runo,

Margaret Karembu,

Francis Nan'gayo

et al.

Global Food Security, Journal Year: 2024, Volume and Issue: 42, P. 100785 - 100785

Published: July 24, 2024

Genome editing has great potential to alleviate the persistent food insecurity in Africa. However, achieving this goal is faced with a myriad of challenges. We describe components that we envisage are crucial positioning Africa for an early commercial agricultural genome take-off. review evolving technologies based on CRISPR/Cas systems. then present status research improve sustainability Africa, and its commercialization short-term.

Language: Английский

Citations

6
One-step knock-in of two antimicrobial peptide transgenes at multiple loci of catfish by CRISPR/Cas9-mediated multiplex genome engineering DOI
Jinhai Wang,

Indira Medina Torres,

Mei Shang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 260, P. 129384 - 129384

Published: Jan. 21, 2024

Language: Английский

Citations

5