Transcriptome analysis reveals candidate genes for different root types of alfalfa in response to drought stress DOI Creative Commons
Kun Wang, Lili Nan,

Jing Xia

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Abstract Background We aimed to gain insight into the response mechanism of alfalfa drought stress by recognizing and analyzing drought-responsive genes in roots different root types alfalfa. The rhizomatous-rooted M. sativa ‘Qingshui’ (or QS), tap-rooted ‘Longdong’ LD), creeping-rooted varia ‘Gongnong No. 4’ GN) were used simulate with PEG-6000 analyze transcriptome information physiological characteristics system. Results It was found that aridity caused a significant increase content osmotic substances antioxidant enzyme activity. malondialdehyde (MDA) QS lower than LD GN under conditions, indicating stronger accumulation capacity regulatory substances. Based on sequencing results, 14,475, 9,336, 9,243 upregulated DEGs from QS, LD, annotated 26, 29, 28 transcription factor families, respectively. showed more GN. KEGG enrichment analysis identified significantly enriched metabolic pathways such as amino acid biosynthesis, phenylpropanoid plant hormone signaling transduction, MAPK pathways. This suggests strong correlation between these stress. Simultaneously, it discovered associated ABA (MS. gene93372, MS. gene072046, gene012975) are crucial for adapting Conclusions These genes, serine/threonine protein kinases abscisic receptors, play role They could serve potential candidate resistance research alfalfa, providing molecular foundation studying resistance.

Language: Английский

Transcriptome analysis reveals candidate genes for different root types of alfalfa (Medicago sativa) after water stress induced by PEG-6000 DOI Creative Commons
Kun Wang, Lili Nan,

Jing Xia

et al.

Chemical and Biological Technologies in Agriculture, Journal Year: 2024, Volume and Issue: 11(1)

Published: Aug. 5, 2024

We aimed to gain insight into the response mechanism of alfalfa (Medicago sativa) drought stress by recognizing and analyzing drought-responsive genes in roots different root types alfalfa. The rhizomatous-rooted M. sativa cv.'Qingshui' (QS), tap-rooted cv.'Longdong' (LD), creeping-rooted varia cv. 'Gongnong No. 4' (GN) were used analyze transcriptome information physiological characteristics systems cultivars under simulated using PEG-6000. It was found that aridity caused a significant increase content osmotic substances antioxidant enzyme activity. malondialdehyde (MDA) QS lower than LD GN moisture stress, indicating stronger accumulation capacity regulatory substances. Based on sequencing results, 14,475, 9336, 9243 upregulated DEGs from QS, LD, annotated 26, 29, 28 transcription factor families, respectively. showed more GN. KEGG enrichment analysis identified significantly enriched metabolic pathways such as amino acid biosynthesis, phenylpropanoid plant hormone signaling transduction, MAPK pathways. This suggests strong correlation between these stress. results also show associated with ABA (MS. gene93372, MS. gene072046, gene012975) are crucial for plant's adaptation These genes, serine/threonine protein kinases abscisic receptors, play role They could serve potential candidate resistance research alfalfa, providing molecular foundation studying resistance.

Language: Английский

Citations

2
Multifaceted natural drought response mechanisms in three elite date palm cultivars uncovered by expressed sequence tags analysis DOI Creative Commons
Zafar Iqbal, Muhammad Munir

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 5, 2024

This study extends our prior research on drought responses in three date palm cultivars (Khalas, Reziz, and Sheshi) under controlled conditions. Here, we investigated their stress adaptive strategies ambient environment. Under natural field conditions, experienced significantly (p ≤ 0.05) varying regulations physiological attributes. Specifically, chlorophyll content, leaf RWC, photosynthesis, stomatal conductance, transpiration reduced significantly, while intercellular CO2 concentration water use efficiency increased. Through suppression subtraction hybridization (SSH), a rich repertoire (1026) of drought-responsive expressed sequence tags (ESTs) were identified: 300 Khalas, 343 383 Sheshi. Functional analysis ESTs, including gene annotation KEGG pathways elucidation, unveiled that these withstand by leveraging indigenous multifaceted pathways. While some aligned with previously reported resilience mechanism observed several new noted, pinpointing cultivar-specific adaptations. ESTs identified enriched through GSEA analysis. Khalas exhibited enrichment cellular metabolic processes, catalytic activity, metal ion binding. Reziz showed biological regulation, signaling, nuclear functions. Conversely, Sheshi displayed organelle, photosynthetic, ribosomal components. Notably, ca. 50% the unique novel, underlining complexity genetic toolkit. Overall, superior tolerance, followed Sheshi, highlighting variability adaptation. Conclusively, diverse to cope drought, demonstrating greater compared settings.

Language: Английский

Citations

1
Agronomic Performance and Technological Attributes of Sugarcane Cultivars Under Split-Irrigation Management DOI Creative Commons
Henrique Fonseca Elias de Oliveira, Fernando Henrique Arriel, Frederico Antônio Loureiro Soares

et al.

AgriEngineering, Journal Year: 2024, Volume and Issue: 6(4), P. 4337 - 4352

Published: Nov. 18, 2024

In addition to being an important instrument in the search for increasingly greater productivity, agricultural production with adequate use of irrigation systems significantly minimizes impact on water resources. To meet high productivity and yield, as well industrial quality, a series studies sugarcane cultivation are necessary. Despite able adapt drought, is still crop highly dependent guarantee best quality standards. Our study aimed analyze agronomic performance technological attributes two cultivars, evaluating vegetative productive pattern, cultivars RB92579 SP80–1816, which were cultivated under split-irrigation management Sugarcane Research Unit IF Goiano—Campus Ceres, located state Goiás Central-West region Brazil. A self-propelled sprinkler system (IrrigaBrasil) was used, duly equipped Twin 120 Komet sprinklers (Fremon, USA). The propagated vegetatively planted 0.25 m deep furrows 1.5 between rows. experiment conducted completely randomized design (CRD), bifactorial split-plot scheme (5 × 2), four replications, where experimental plots subjected one following five systems: 00 mm + mm; 20 40 30 or 60 mm. At 150 days after planting (DAP), respective applied: Biometric attributes, such plant height (PH) stem diameter (SD), evaluated this case at 30-day intervals, starting 180 DAP ending 420 DAP. Measurements soluble solids content (°Brix), apparent sucrose (POL), fiber (Fiber), juice purity (PZA), broth POL (BP), reducing sugars (RS), total recoverable (TRS) made by sampling stems harvest showed sugar contents 11.89% 8.86% higher than those recorded SP80–1816 mm, respectively. Shoot reached 187.15 t ha−1 42.16% shoot observed SP80–1816. Both qualitative quantitative indices treatments that applied volumes initial phase culture, coinciding dry season. cultivar better adaptation prevailing conditions cultivar.

Language: Английский

Citations

1
Identification of the Gossypium hirsutum SDG Gene Family and Functional Study of GhSDG59 in Response to Drought Stress DOI Creative Commons
Ziyu Wang,

Wanwan Fu,

Xin Zhang

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(9), P. 1257 - 1257

Published: April 30, 2024

SET-domain group histone methyltransferases (SDGs) are known to play crucial roles in plant responses abiotic stress. However, their specific function cotton’s response drought stress has not been well understood. This study conducted a comprehensive analysis of the SDG gene family Gossypium hirsutum, identifying total 82 genes. An evolutionary revealed that can be divided into eight subgroups. The expression shows some GhSDG genes preferentially expressed tissues, indicating involvement cotton growth and development. transcription level is induced by PEG, with GhSDG59 showing significant upregulation upon polyethylene glycol (PEG) treatment. Quantitative polymerase chain reaction (qPCR) showed accumulation transcripts was significantly upregulated under Further functional studies using virus-induced silencing (VIGS) reduced tolerance Under conditions, proline content, superoxide dismutase (SOD) peroxidase (POD) enzyme activities GhSDG59-silenced plants were lower than control plants, while malondialdehyde (MDA) content higher. Transcriptome sequencing led changes levels 1156 KEGG enrichment these differentially (DEGs) mainly enriched carbon metabolism starch sucrose pathways. annotation identified drought-responsive genes, such as ERF, CIPK, WRKY, among DEGs. indicates involved drought-stress affecting related pathways, provides valuable information for genomic SDGs highlights potential beneficial genetic improvement breeding cotton.

Language: Английский

Citations

0
Transcriptome analysis reveals candidate genes for different root types of alfalfa in response to drought stress DOI Creative Commons
Kun Wang, Lili Nan,

Jing Xia

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Abstract Background We aimed to gain insight into the response mechanism of alfalfa drought stress by recognizing and analyzing drought-responsive genes in roots different root types alfalfa. The rhizomatous-rooted M. sativa ‘Qingshui’ (or QS), tap-rooted ‘Longdong’ LD), creeping-rooted varia ‘Gongnong No. 4’ GN) were used simulate with PEG-6000 analyze transcriptome information physiological characteristics system. Results It was found that aridity caused a significant increase content osmotic substances antioxidant enzyme activity. malondialdehyde (MDA) QS lower than LD GN under conditions, indicating stronger accumulation capacity regulatory substances. Based on sequencing results, 14,475, 9,336, 9,243 upregulated DEGs from QS, LD, annotated 26, 29, 28 transcription factor families, respectively. showed more GN. KEGG enrichment analysis identified significantly enriched metabolic pathways such as amino acid biosynthesis, phenylpropanoid plant hormone signaling transduction, MAPK pathways. This suggests strong correlation between these stress. Simultaneously, it discovered associated ABA (MS. gene93372, MS. gene072046, gene012975) are crucial for adapting Conclusions These genes, serine/threonine protein kinases abscisic receptors, play role They could serve potential candidate resistance research alfalfa, providing molecular foundation studying resistance.

Language: Английский

Citations

0