The impact of Annona muricata and metformin on semen quality and hormonal profile in Arsenic trioxide-induced testicular dysfunction in male Wistar rat DOI Creative Commons
Akpoghene Eyeghre Onoriode,

Ezeokafor E.N Dike C.C,

Oparaji K.C

et al.

Magna Scientia Advanced Research and Reviews, Journal Year: 2023, Volume and Issue: 8(1), P. 001 - 018

Published: May 5, 2023

Male Infertility has been an issue of significant concern affecting appraised 20-30% couples worldwide. The study aims to access the hormonal and semen profile following metformin ethanolic leave extract Annona muricata (ELAM) on arsenic-induced toxicity in Wistar rats. Thirty-male rats were used study, weighing 150-170 grams, divided into six groups five each. Group A received 50 mg/kg arsenic trioxide (As2O3) only. B feed water ad libitum. Groups C D 50mg/kg As2O3 immediately treated with 30 500 ELAM. E 500mg/kg ELAM As2O3, group F As2O3. Data obtained subjected ANOVA followed by post Hoc LSD using SPSS version 25. Semen quality, Testosterone, FSH, LH levels, gonadal-somatic index analysed. Values presented as Mean±SEM considered at p<0.05. revealed a decrease D, while B, C, E, had no compared A. showed impaired sperm motility, morphology, total count significantly improved count. LH, FSH decline except for LH. increase testosterone FSH. concluded that MET quality profile, which could be reversing prevention male infecundity linked caused oxidative stress.

Language: Английский

Antenatal substance exposure and infants’ telomere length: A scoping review DOI Creative Commons
Holly Wei,

Lori A. Devlin,

Ya Cao

et al.

Global Pediatrics, Journal Year: 2025, Volume and Issue: unknown, P. 100252 - 100252

Published: March 1, 2025

Language: Английский

Citations

0
Reproductive aging and telomeres: Are women and men equally affected? DOI
Lucía Chico-Sordo, Isabel Córdova-Oriz, Alba M. Polonio

et al.

Mechanisms of Ageing and Development, Journal Year: 2021, Volume and Issue: 198, P. 111541 - 111541

Published: July 7, 2021

Language: Английский

Citations

22
Is telomere length a biomarker of sperm quality? A systematic review and meta‐analysis of observational studies DOI Creative Commons
María Fernández de la Puente, Albert Salas‐Huetos, Cristina Valle‐Hita

et al.

Andrology, Journal Year: 2023, Volume and Issue: 12(2), P. 277 - 288

Published: June 17, 2023

Telomeres are essential for the integrity of chromosome ends during cell division and their involvement in different processes linked to aging has been established. These components involved spermatogenesis seem play an important role fertilization embryo development. Telomere length is shortened with each division. Recently, short sperm telomere proposed as a potential biomarker male infertility.

Language: Английский

Citations

7
Association between sperm mitochondrial DNA copy number and deletion rate and industrial air pollution dynamics DOI Creative Commons
Miluše Vozdová, Svatava Kubı́čková,

Věra Kopecká

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: May 18, 2022

Abstract The effects of air pollution on men’s reproductive health can be monitored by evaluating semen quality and sperm DNA damage. We used real-time PCR to analyse the mitochondrial copy number (mtDNAcn) deletion (mtDNAdel) rates in samples collected from 54 men two seasons with different levels industrial traffic pollution. MtDNAdel were significantly higher following high exposure period positively correlated mtDNAcn. However, we did not find any difference mtDNAcn between seasons. MtDNAcn was fragmentation index chromatin condensation defects, previously assessed structure assay, negatively concentration, progressive motility, viability, normal morphology. This indicates that is more closely associated male fertility than mtDNAdel rates. In contrast, might a sensitive biomarker urban environments.

Language: Английский

Citations

9
Freezing Does Not Alter Sperm Telomere Length despite Increasing DNA Oxidation and Fragmentation DOI Open Access

Charlène Gouhier,

Hanae Pons‐Rejraji, Sandra Dollet

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(5), P. 1039 - 1039

Published: May 3, 2023

Correlations were reported between sperm telomere length (STL) and male fertility, DNA fragmentation, oxidation. Sperm freezing is widely used for assisted reproductive techniques, fertility preservation, donation. However, its impact on STL remains unknown. For this study, semen surplus from patients who underwent routine analysis used. The of slow was analyzed by performing qPCR before after freezing. populations with different evaluated using Q-FISH. relationship oxidation, assessed in fresh frozen samples. No significant observed, neither measured nor Q-FISH allowed the distinguishing STLs within individual Slow induced distributions some samples, but no correlation found fragmentation or does not alter despite increasing oxidation fragmentation. As alterations could be transmitted to offspring, lack method ensures safety procedure.

Language: Английский

Citations

5
Sperm telomere length in male-factor infertility and reproduction DOI Creative Commons
Zane Randell, Hojat Dehghanbanadaki, Kiarad Fendereski

et al.

Fertility and Sterility, Journal Year: 2023, Volume and Issue: 121(1), P. 12 - 25

Published: Nov. 9, 2023

Language: Английский

Citations

5
Telomere Length and Telomerase Activity of Granulosa Cells and Follicular Fluid in Women Undergoing In Vitro Fertilization DOI Creative Commons

Sándor Péntek,

Ákos Várnagy, Bálint Farkas

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(2), P. 419 - 419

Published: Feb. 8, 2023

This study aimed to evaluate the interrelationship between telomere length, telomerase activity and oxidative DNA damage in patients undergoing vitro fertilization (IVF). single-center, observational clinical comprised 102 unselected, consecutive with various infertility diagnoses. Granulosa cells (GCs) follicular fluid (FF) were analyzed simultaneously for functions marker of damage, 8-hydroxy-2-deoxyguanosine (8-OHdG). An Absolute Human Telomere Lengths Quantification qPCR Assay kit Telomerase Activity (Nucleotestbio, Budapest, Hungary), as well an 8-OHdG ELISA (Abbexa Ltd., Cambridge, United Kingdom) used analyses. Similar lengths found GCs FF, however was markedly depressed, while levels elevated FF compared those (p < 0.01). independent both FF. However, GC inversely related 0.05). Importantly, had significant negative impact on number retrieved MII oocytes 0.01), whereas negatively further fertilized blastocysts In conclusion, we could not confirm direct association function reproductive potential. mainly reflected by 8-OHdG, adversely affected early markers IVF outcome pregnancies.

Language: Английский

Citations

4
Telomeres and SIRT1 as Biomarkers of Gamete Oxidative Stress, Fertility, and Potential IVF Outcome DOI Open Access
Anna Pańczyszyn, Ewa Boniewska‐Bernacka, Iwona Wertel

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8652 - 8652

Published: Aug. 8, 2024

The number of infertile couples undergoing in vitro fertilisation (IVF) has increased significantly. efficacy this procedure is contingent upon a multitude factors, including gamete quality. One factor influencing quality oxidative stress, which leads to telomere damage and accelerates cellular ageing. Identifying new biomarkers that can predict the success assisted reproduction techniques current relevant area research. In review, we discuss potential role SIRT1, protein known protect against stress telomeres, are responsible for genome stability, as technique outcomes.

Language: Английский

Citations

1
Cyclophosphamide‐Induced Infertility and the Impact of Antioxidants DOI
Morteza Abdi, Manouchehr Fadaee, Amirreza jourabchi

et al.

American Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 92(6)

Published: Dec. 1, 2024

ABSTRACT An important drawback of anticancer chemotherapy is the harm it causes to healthy cells. Cyclophosphamide (CP) a widely used chemotherapeutic alkylating agent that regularly in cancer treatment. However, can cause severe side effects, including genotoxicity, due its ability damage DNA. This toxicity thought be associated with oxidative stress induced by an excessive amount reactive oxygen species (ROS). Therefore, there specific focus on potential effects treatments fertility. Due increasing life expectancy patients, those desiring parenthood may face negative impacts therapies. Utilizing substances antioxidant and cytoprotective characteristics protect reproductive system from harmful consequences during would highly beneficial. review introduces physiological pathological roles ROS systems both males females, then we address adverse CP administration infertility discuss how antioxidants reverse these effects.

Language: Английский

Citations

1
Genetic Association in the Maintenance of the Mitochondrial Microenvironment and Sperm Capacity DOI Creative Commons
Hwang I. S. Thomas, Ying‐Shiuan Chen, Ching-Han Hung

et al.

Oxidative Medicine and Cellular Longevity, Journal Year: 2021, Volume and Issue: 2021(1)

Published: Jan. 1, 2021

Sperm motility is one of the major determinants male fertility. Since sperm need a great deal energy to support their fast movement by active metabolism, they are thus extremely vulnerable oxidative damage reactive oxygen species (ROS) and other free radicals generated as byproducts in electron transport chain. The present study aimed at understanding impact mitochondrial oxidizing/reducing microenvironment etiopathology infertility. We detected DNA (mtDNA) 4,977 bp deletion human sperm. examined gene mutation ATP synthase 6 ( ATPase6 m.T8993G) generation, polymorphisms uncoupling protein 2 UCP2 , G‐866A) phosphorylation, role genes such manganese superoxide dismutase MnSOD C47T) catalase CAT C‐262T) scavenging system neutralizing species, 8‐oxoguanine glycosylase hOGG1 C1245G) 8‐hydroxy‐2 ′ ‐deoxyguanosine (8‐OHdG) repair. found that with higher were have membrane potential bioenergetics. genotype frequencies G‐866A, C47T, C‐262T be significantly different among fertile subjects, infertile subjects more than 50% motility, less motility. A prevalence mtDNA was impaired Furthermore, we there significant differences between occurrences (C47T) (C1245G). In conclusion, maintenance redox genome integrity an important issue

Language: Английский

Citations

8