Extracellular Vesicles from Plasma of Patients with Glioblastoma Promote Invasion of Glioblastoma Cells Even After Tumor Resection DOI Creative Commons
Ekaterina N. Lyukmanova, Artem V. Kirichenko, Igor Medyanik

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2834 - 2834

Published: Dec. 13, 2024

Background: Glioblastoma (GB) is a highly aggressive tumor, whose progression mediated by secretion of extracellular vesicles (EVs), which can pass the brain–blood barrier and be found in plasma. Here, we performed comparative analysis effects EVs from plasma healthy donors (hEVs) GB patients before (bEVs) after (aEVs) tumor surgical resection on invasion normal astrocytes cells. Methods: We transwell assay, analyzed MAP kinases activation Western blotting, studied SNAI1/SNAI2 cellular localization confocal microscopy, measured cadherins expression flow cytometry, cytokines, regulate migration inflammation, immunoassay. Results: hEVs did not affect cells, there was down-regulated astrocytes, while increased E- N-cadherin inflammation adhesion regulators both bEVs enhanced cells but via AKT, JNK1/2/3, p38 activation, stimulated clasterization SNAI1 cell nucleus, promoted an E/N cadherin switch, caused aEVs exhibited most pro-oncogenic (stimulation invasion, nuclear localization, JNK1/2/3 cells). However, were less pronounced than those bEVs. Conclusions: In our study, revealed common different plasma-derived hEVs, aEVs, stimulate some Being tumorigenic then bEVs, are still able to promote probably remaining resection.

Language: Английский

Extracellular Vesicles from Plasma of Patients with Glioblastoma Promote Invasion of Glioblastoma Cells Even After Tumor Resection DOI Creative Commons
Ekaterina N. Lyukmanova, Artem V. Kirichenko, Igor Medyanik

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(12), P. 2834 - 2834

Published: Dec. 13, 2024

Background: Glioblastoma (GB) is a highly aggressive tumor, whose progression mediated by secretion of extracellular vesicles (EVs), which can pass the brain–blood barrier and be found in plasma. Here, we performed comparative analysis effects EVs from plasma healthy donors (hEVs) GB patients before (bEVs) after (aEVs) tumor surgical resection on invasion normal astrocytes cells. Methods: We transwell assay, analyzed MAP kinases activation Western blotting, studied SNAI1/SNAI2 cellular localization confocal microscopy, measured cadherins expression flow cytometry, cytokines, regulate migration inflammation, immunoassay. Results: hEVs did not affect cells, there was down-regulated astrocytes, while increased E- N-cadherin inflammation adhesion regulators both bEVs enhanced cells but via AKT, JNK1/2/3, p38 activation, stimulated clasterization SNAI1 cell nucleus, promoted an E/N cadherin switch, caused aEVs exhibited most pro-oncogenic (stimulation invasion, nuclear localization, JNK1/2/3 cells). However, were less pronounced than those bEVs. Conclusions: In our study, revealed common different plasma-derived hEVs, aEVs, stimulate some Being tumorigenic then bEVs, are still able to promote probably remaining resection.

Language: Английский

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