Bioactive Molecules from the Invasive Blue Crab Callinectes sapidus Exoskeleton: Evaluation of Reducing, Radical Scavenging, and Antitumor Activities

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(1), P. 45 - 45

Published: Jan. 17, 2025

In recent years, the invasive Atlantic blue crab (Callinectes sapidus) has increased its spread throughout Mediterranean Sea, threatening native biodiversity and local economies. This study aimed to valorize C. sapidus sampled in Sicily by utilizing exoskeleton as a source of chitosan, astaxanthin, bio-phenolic compounds. These biomolecules were evaluated for their reducing, radical scavenging, antitumor activity. The ferric ion reducing antioxidant power (FRAP) free scavenging activity against 2,2-Diphenyl-1-picrylhydrazyl (DPPH) significantly higher chitosan (3.16 ± 0.10 mg AAE/g 8.1 µmol TE/g). No significant differences observed among tested 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Both compounds astaxanthin exhibited dose-dependent cytotoxicity on CaCo-2 (IC50 = 12.47 18 µg/mL) HepG2 10.25 1.26 cell lines, while only bio-phenols showed no cytotoxic effect differentiated cells up 20 µg/mL. findings highlight value by-products supporting circular economy, offering sustainable approach managing this species providing bioactive with promising medical nutraceutical applications.

Language: Английский

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Important carotenoids derived from marine biomass: Extraction, stabilization, and potentiality in food, cosmetics, and pharmaceutical application

Food Bioscience, Journal Year: 2024, Volume and Issue: 60, P. 104421 - 104421

Published: May 27, 2024

Language: Английский

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Bioactive Compounds Extraction from Crustacean Waste by Biotechnology Process

Published: Jan. 1, 2025

Language: Английский

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Astaxanthin extraction from lobster “Panulirus penicillatus” waste and its quantification by environmentally safe microbial methods

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Journal Year: 2025, Volume and Issue: 77(3)

Published: Jan. 1, 2025

ABSTRACT The accumulation of large amounts crustacean waste is a major environmental issue, however, this can yield valuable bioactive chemicals. Carotenoids and astaxanthin were recovered from the exoskeleton red sea lobsters (Panulirus penicillatus) utilizing three eco-friendly techniques. techniques employed include use flaxseed oil at various incubation durations, beneficial bacterial fungal strains (Lactobacillus lactis, Bifidobacterium Saccharomyces cerevisiae, Candida utilis) in biological method, utilization microorganisms with oil. spectrophotometer HPLC results showed that after one hour incubation, highest amount carotenoid achieved extraction was 0.52g/g 13.4g/g, respectively. Also, quantities technique using S. cerevisiae 0.7g/g 30.766g/g, last approach yielded (9.39g/g for 46.266g/g carotenoid). It critical to develop more environmentally acceptable technologies extracting chemicals decrease contamination future. extensive research enhance efficiency will eventually minimize need

Language: Английский

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Valorization of the Invasive Blue Crabs (Callinectes sapidus) in the Mediterranean: Nutritional Value, Bioactive Compounds and Sustainable By-Products Utilization

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(9), P. 430 - 430

Published: Sept. 23, 2024

The blue crab (Callinectes sapidus), originally from the western Atlantic Ocean, has recently spread to Mediterranean and is now considered one of hundred most invasive species in that region. This opportunistic species, known for its adaptability different temperatures salinities, negatively impacts biodiversity human activities such as fishing tourism Mediterranean. However, gaining interest a potential food resource due high nutritional value delicate, sweet flavor. Its meat rich protein (14% 30%), omega-3 fatty acids (EPA DHA) other essential nutrients beneficial health vitamins, minerals. Utilizing this production new foods could help mitigate negative impact invasiveness offer economic opportunities. One challenge with generation waste. Approximately 6–8 million tonnes shells are produced worldwide each year, leading disposal problems concerns regarding environmental sustainability. To improve sustainability, there need valorize these residues, which an important source proteins, lipids, chitin, minerals, pigments can be processed into high-value-added products. especially areas industrial pollution, attention should paid heavy metal (Cd As) contents shells. Studies suggest by-products used various sectors, reducing impacts, promoting circular economy, creating

Language: Английский

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The Blue Treasure: Comprehensive Biorefinery of Blue Crab (Callinectes sapidus)

Foods, Journal Year: 2024, Volume and Issue: 13(13), P. 2018 - 2018

Published: June 26, 2024

The blue crab, Callinectes sapidus (Rathbun, 1896), has become an invading species in the Mediterranean region, almost completely replacing native within a few years and causing significant loss to local production. In some areas, there is urgent need propose new supply chains based on crab exploitation, where potential valorisation routes for unsaleable waste play important role. final purpose transform threat into treasure, towards more sustainable world. addition applications food industries, considerable quantity of bioactive compounds by-products, such as polysaccharides, proteins, amino acids, carotenoids, chitin, needs be capitalised by means efficacious strategies appropriate management. Crab exoskeleton can also exploited carbonaceous material with several fields, including medicine. Blue molecules have been widely recognised having antioxidant, anticancer, antidiabetic, anti-inflammatory, antimicrobial properties. Due these functional distinctive activities, high-value components could employed various industries food–feed–pharma cosmetics. Recycling reusing underutilised but economically valuable or by-products help reduce environmental impacts whole chain from perspective circular economy.

Language: Английский

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Extraction and Synthesis of Typical Carotenoids: Lycopene, β-Carotene, and Astaxanthin

Molecules, Journal Year: 2024, Volume and Issue: 29(19), P. 4549 - 4549

Published: Sept. 25, 2024

Carotenoids are tetraterpene compounds acting as precursors to vitamin A, with functions that include protecting eyesight, enhancing immunity, promoting cell growth and differentiation, providing antioxidative benefits. Lycopene, β-carotene, astaxanthin particularly critical for health have diverse applications in food, products, medicine. However, natural carotenoids encased within structures, necessitating mechanical methods disrupt the wall their extraction purification—a process often influenced by environmental conditions. Thus, improving efficiency of carotenoid from resources is great interest. This review delves into research progress made on processes, biological carotenoids, focusing lycopene, astaxanthin. Traditional primarily involve organic solvent-assisted crushing. With deeper technological advancements, more environmentally friendly solvents, advanced machinery, suitable being employed enhance purification carotenoids. These improvements significantly increased efficiency, reduced preparation time, lowered production costs, laying groundwork new product developments.

Language: Английский

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A comprehensive review on marine by-products use for the recovery of value-added products

Current Opinion in Green and Sustainable Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 100972 - 100972

Published: Sept. 1, 2024

Language: Английский

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Advances in extraction and biological activities of crawfish chitosan and its application in decolorization of synthetic dyes

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Journal Year: 2024, Volume and Issue: 76(6)

Published: Jan. 1, 2024

ABSTRACT An eco-friendly method of extraction for chitin and chitosan extracted from crawfish was our goal. Chitin is always present with proteins, minerals, other components. This study used an eco-accommodating, novel technique extraction. Lactobacillus lactis the deproteinization demineralization in a single stage by Saccharomyces cerevisiae (BB: biological-biological extraction) to convert into chitosan. BB more environmentally friendly producing than deacetylation NaOH (BC: biological-chemical extraction). Chitosan characterized FTIR. A high degree (%) observed. The UV spectrum similar at 0.788, 0.415, 1.150 CC, BC, BB, respectively. results show that (BB) has potential applications biomedical fields such as antioxidant activity, anticancer activity against human liver cancer (HepG2), breast (MCF-7) hepatocellular carcinoma (HCT) cell lines. terms water treatment removal dyes using are valuable its application industrial wastewater demonstrate it can be biosorbent.

Language: Английский

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Extraction and Purification of Highly Active Astaxanthin from Corynebacterium glutamicum Fermentation Broth

Marine Drugs, Journal Year: 2023, Volume and Issue: 21(10), P. 530 - 530

Published: Oct. 11, 2023

The marine carotenoid astaxanthin is one of the strongest natural antioxidants and therefore used in a broad range applications such as cosmetics or nutraceuticals. To meet growing market demand, producer Corynebacterium glutamicum has been engineered to produce by heterologous expression genes from bacterium Fulvimarina pelagi. exploit this promising source fermentative astaxanthin, an efficient extraction process using ethanol was established study. Appropriate parameters for were identified screening concentration (62.5-97.5% v/v), temperature (30-70 °C) biomass-to-solvent ratio (3.8-19.0 mgCDW/mLsolvent). results demonstrated that optimal conditions were: 90% ethanol, 60 °C, 5.6 mgCDW/mLsolvent. In total, 94% cellular recovered oleoresin obtained contained 9.4 mg/g astaxanthin. With respect other carotenoids, further purification column chromatography resulted pure (100%, HPLC). addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay showed similar activities compared esterified microalgae nine-fold higher antioxidative activity than synthetic

Language: Английский

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