Role of vertical and horizontal microbial transmission of antimicrobial resistance genes in early life: insights from maternal-infant dyads

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 77, P. 102424 - 102424

Published: Jan. 21, 2024

Early life represents a critical window for metabolic, cognitive and immune system development, which is influenced by the maternal microbiome as well infant gut microbiome. Antibiotic exposure, mode of delivery breastfeeding practices modulate reservoir antibiotic resistance genes (ARGs). Vertical horizontal microbial gene transfer during early mechanisms behind these transfers are being uncovered. In this review, we aim to provide an overview current knowledge on in mother–infant dyad through vertical transmission highlight main gaps challenges area.

Language: Английский

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The detailed analysis of the microbiome and resistome of artisanal blue-veined cheeses provides evidence on sources and patterns of succession linked with quality and safety traits

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: April 27, 2024

Abstract Background Artisanal cheeses usually contain a highly diverse microbial community which can significantly impact their quality and safety. Here, we describe detailed longitudinal study assessing the of ripening in three natural caves on microbiome resistome succession across different producers Cabrales blue-veined cheese. Results Both producer cave were ripened influenced cheese microbiome. Lactococcus former Lactobacillus genus, among other taxa, showed high abundance at initial stages ripening, either coming from raw material, starter culture used, and/or environment processing plants. Along caves, these taxa displaced by bacteria, such as Tetragenococcus , Corynebacterium Brevibacterium Yaniella Staphylococcus predominantly originating environments (mainly food contact surfaces), demonstrated source-tracking analysis, strain analysis read level, characterization 613 metagenome-assembled genomes. The koreensis halophilus detected has not been found previously metagenomes. Furthermore, level horizontal gene transfer with members microbiome, mainly involving genes related to carbohydrate metabolism functions. revealed that milk associated are rich reservoir antimicrobial resistance determinants, aminoglycosides, tetracyclines, β -lactam antibiotics harbored aerobic gram-negative bacteria relevance safety point view, Escherichia coli Salmonella enterica Acinetobacter Klebsiella pneumoniae displacement most milk-associated cave-associated during gave rise significant decrease load ARGs and, therefore, safer end product. Conclusion Overall, represented an important source non-starter microorganisms may play relevant role products. Among them, have identified novel regarded being dominant components ( spp.), providing very valuable information for authentication this protected designation origin artisanal

Language: Английский

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Class 1 integron and related antimicrobial resistance gene dynamics along a complex freshwater system affected by different anthropogenic pressures

Environmental Pollution, Journal Year: 2022, Volume and Issue: 316, P. 120601 - 120601

Published: Nov. 6, 2022

Language: Английский

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Integrons as the potential targets for combating multidrug resistance inEnterobacteriaceaeusing CRISPR- Cas9 technique

Journal of Applied Microbiology, Journal Year: 2023, Volume and Issue: 134(7)

Published: July 1, 2023

Abstract The emergence of multi-drug resistance (MDR) to pan-drug (PDR) in Enterobacteriaceae has made treatment extremely challenging. Genetic mutations and horizontal gene transfer (HGT) through mobile genetic elements (MGEs) were frequently associated mechanisms drug pathogens. However, transposons, plasmids, integrons MDR genes bacterium via HGT much faster. Integrons are dsDNA segment that plays a crucial role the adaptation evolution bacteria. They contain multiple cassettes code for antibiotic determinants expressed by single promoter (Pc). cause Enterobacteriaceae. Although alternatives antibiotics such as bacteriophages, phage proteins, antimicrobial peptides, natural compounds have been widely used treat infections, there limited efforts reverse ability Thus, silencing harboured on MGEs achieved Gene Editing Techniques (GETs) might prevent spread MDR. One GETs, which simple design, good repeatability, low cost, high efficiency, is CRISPR- Cas9 system. this review first kind focuses utilizing structure an integron make it ideal target GETs like systems.

Language: Английский

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Comparison of antimicrobial resistant Escherichia coli isolated from Irish commercial pig farms with and without zinc oxide and antimicrobial usage

Gut Pathogens, Journal Year: 2023, Volume and Issue: 15(1)

Published: Feb. 24, 2023

The prophylactic use of antimicrobials and zinc oxide (ZnO) in pig production was prohibited by the European Union 2022 due to potential associations between antimicrobial heavy metal usage with resistance (AMR) concerns regarding environmental pollution. However, effects their on bacterial AMR profiles commercial farms are still not fully understood previous studies examining effect ZnO have reported contrasting findings. objective this study examine farms. Faecal samples were taken 10 Irish farms, which 5 regularly used (amoxicillin or sulphadiazine-trimethoprim) for prevention disease. other did any form prophylaxis. Escherichia coli numbers quantified from all using non-supplemented supplemented Tryptone Bile X-glucuronide agar.In total 351 isolates phenotypically analysed, genomes 44 AmpC/ESBL-producing E. 4 characterised whole-genome sequencing. Phenotypic analysis suggested higher multi-drug resistant (MDR) Furthermore, prophylaxis associated apramycin, trimethoprim, tetracycline, streptomycin, chloramphenicol, while ciprofloxacin more Thirty-four strains harboured blaCTX-M-1 gene multi drug (MDR). Moreover, network plasmids integrons showed that biocide genes frequently co-located mobile genetic elements, indicating possibility co-selection during as a contributor occurrence persistence farms.The results evidence treatment pigs post-weaning can favour selection development MDR coli. Co-location elements observed. This demonstrated usefulness phenotypic genotypic detection combining sequencing microbiological methods.

Language: Английский

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