Advances in DNA-based electrochemical biosensors for the detection of foodborne pathogenic bacteria

Talanta, Journal Year: 2024, Volume and Issue: 275, P. 126072 - 126072

Published: April 16, 2024

Language: Английский

---

Recent Advances in Hydrogel-Based Biosensors for Cancer Detection

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(36), P. 46988 - 47002

Published: Aug. 27, 2024

Early cancer detection is crucial for effective treatment, but current methods have limitations. Novel biomaterials, such as hydrogels, offer promising alternatives developing biosensors detection. Hydrogels are three-dimensional and cross-linked networks of hydrophilic polymers that properties similar to biological tissues. They can be combined with various achieve high sensitivity, specificity, stability. This review summarizes the recent advances in hydrogel-based detection, their synthesis, applications, challenges. It also discusses implications future directions this emerging field.

Language: Английский

---

Current trends and future potential in the detection of avian coronaviruses: An emphasis on sensors-based technologies.

Virology, Journal Year: 2025, Volume and Issue: 604, P. 110399 - 110399

Published: Jan. 18, 2025

Infectious bronchitis virus (IBV), an avian coronavirus, member of the genus Gammacoronavirus, poses significant threats to poultry health, causing severe respiratory, reproductive, and renal infections. The genetic diversity IBV, driven by mutations, recombination deletions, has led emergence numerous serotypes genotypes, complicating both diagnosis control measures. Rapid accurate diagnostic tools are essential for effective disease management minimizing economic losses. Conventional methods, such as PCR, isolation, serological assays, hindered limitations in sensitivity, specificity, turnaround time. In contrast, innovative biosensor platforms employing advanced detection mechanisms-including electrochemical, optical, piezoelectric sensors-offer a transformative solution. These technologies provide portable, highly sensitive, rapid IBV detection. Beyond addressing challenges conventional these biosensor-based approaches facilitate real-time monitoring enhance surveillance. This review highlights potential biosensors their integration into strategies coronavirus infections, presenting them promising alternative precise efficient

Language: Английский

---

Sensitive and on-Site Detection of Staphylococcus aureus Based on CRISPR/Cas 13a-Assisted Chemiluminescence Resonance Energy Transfer

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(22), P. 9270 - 9277

Published: May 21, 2024

Developing a specific, sensitive, rapid, and on-site method for detecting pathogenic bacteria in food samples is critical to ensuring public safety. This article demonstrates CRISPR/Cas13a system chemiluminescence resonance energy transfer (CRET) (CRISPR/Cas 13a-assisted CRET)-based strategy sensitive detection of real samples. Once the hybrid double strand aptamerS. aureus-cRNA recognizes target model Staphylococcus aureus (S. aureus), released cRNA would bind with CRISPR/Cas 13a form complex cRNA-CRISPR/Cas 13a, which could cleave RNA molecule probe horseradish peroxidase (HRP) modified-gold nanoparticles (AuNPs) linked by (AuNPs-RNA-HRP), resulting an enhanced signal due CRET "OFF" phenomenon after introducing substrate luminol. The successfully detected S. drinking water milk limits 20 30 cfu/mL, respectively, within recovery 90.07–105.50%. Furthermore, integrating immunochromatographic test strip (ICTS), achieved as low 102 cfu/mL via smartphone, about 10 times lower than that previously reported AuNPs-based colorimetric ICTS, demonstrating convenient

Language: Английский

---

The importance, benefits, and future of nanobiosensors for infectious diseases

Biotechnology and Applied Biochemistry, Journal Year: 2024, Volume and Issue: 71(2), P. 429 - 445

Published: Jan. 18, 2024

Abstract Infectious diseases, caused by pathogenic microorganisms such as bacteria, viruses, parasites, or fungi, are crucial for efficient disease management, reducing morbidity and mortality rates controlling spread. Traditional laboratory‐based diagnostic methods face challenges high costs, time consumption, a lack of trained personnel in resource‐poor settings. Diagnostic biosensors have gained momentum potential solution, offering advantages low cost, sensitivity, ease use, portability. Nanobiosensors promising tool detecting diagnosing infectious diseases coronavirus disease, human immunodeficiency virus, hepatitis. These sensors use nanostructured carbon nanotubes, graphene, nanoparticles to detect specific biomarkers pathogens. They operate through mechanisms like the lateral flow test platform, where sample containing biomarker pathogen is applied strip. If present, binds recognition probes on strip, indicating positive result. This binding event visualized colored line. review discusses importance, benefits, nanobiosensors diseases.

Language: Английский

---

Electrochemical biosensors for pathogenic microorganisms detection based on recognition elements

Folia Microbiologica, Journal Year: 2024, Volume and Issue: 69(2), P. 283 - 304

Published: Feb. 17, 2024

Language: Английский

---

Development of a New Lab-on-Paper Microfluidics Platform Using Bi-Material Cantilever Actuators for ELISA on Paper

Biosensors, Journal Year: 2023, Volume and Issue: 13(3), P. 310 - 310

Published: Feb. 23, 2023

In this paper, we present a novel and cost-effective lab-on-paper microfluidics platform for performing ELISA autonomously, with no user intervention beyond adding the sample. The utilizes two Bi-Material Cantilever Valves placed in specially designed housing. integration of these valves specific channel network forms complete fluidic logic circuit on paper. housing also incorporates an innovative reagent storage release mechanism that minimizes variability volume reagents released into pads. design was optimized to minimize variance time fluid wicking from pad, using randomized experiment. adheres World Health Organization’s ASSURED principles. used conduct detecting rabbit immunoglobulin G (IgG) buffer, limit detection 2.27 ng/mL quantification 8.33 ng/mL. This represents 58% improvement over previous methods IgG buffer portable microfluidic technology.

Language: Английский

---

MicroRNA biosensors for detection of glioblastoma

Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 556, P. 117829 - 117829

Published: Feb. 13, 2024

Language: Английский

---

Biosensors for plant pathogen detection

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 689 - 710

Published: Jan. 1, 2025

Language: Английский

---

Coupled Cantilever Biosensor Utilizing a Novel Approach to Gap-Method for Real-Time Detection of E. Coli in Low Concentrations

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137665 - 137665

Published: March 1, 2025

Language: Английский

---