Exploring the standardization of human nasal antibody measurements DOI Creative Commons

Xuanxuan Zhang,

Shui Yu, Si Chen

et al.

Emerging Microbes & Infections, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Mucosal immunity is crucial for preventing the infection and transmission of respiratory viruses. Nasal antibody inversely correlated with a lower risk However, current reference standard nasal assessment serum-based, mainly consisting monomeric IgG IgA. The applicability serum-derived standards assessing antibodies, mostly dimeric or polymeric secretory IgA (sIgA), remains unvalidated. Herein, we first proved that sera-derived was not applicable antibodies. Using non-homologous as calibrator introduces systematic error up to 10 times, which does benefit understanding mucosal response. Therefore, attempted develop two candidate (CS1, CS2) using lining fluids (NMLFs) collected from SARS-CoV-2 Omicron convalescents intranasal vaccine recipients, CS3 sIgA monoclonal antibody. CS2 exhibited broad-spectrum binding activity against 12 strains, including all tested subvariants. A collaborative study conducted by seven laboratories demonstrated improved harmonization inter-laboratory variability (pre-standardization geometric coefficients variance, 14-314%; post-standardization, 3-35%). ensures an accurate Thus, established national evaluating SARS-CoV-2-specific antibodies (Lot: 300052-202401, 1000 U/mL). Our work provides benchmark vaccines inspires new avenues developing other vaccines.

Language: Английский

Exploring the standardization of human nasal antibody measurements DOI Creative Commons

Xuanxuan Zhang,

Shui Yu, Si Chen

et al.

Emerging Microbes & Infections, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Mucosal immunity is crucial for preventing the infection and transmission of respiratory viruses. Nasal antibody inversely correlated with a lower risk However, current reference standard nasal assessment serum-based, mainly consisting monomeric IgG IgA. The applicability serum-derived standards assessing antibodies, mostly dimeric or polymeric secretory IgA (sIgA), remains unvalidated. Herein, we first proved that sera-derived was not applicable antibodies. Using non-homologous as calibrator introduces systematic error up to 10 times, which does benefit understanding mucosal response. Therefore, attempted develop two candidate (CS1, CS2) using lining fluids (NMLFs) collected from SARS-CoV-2 Omicron convalescents intranasal vaccine recipients, CS3 sIgA monoclonal antibody. CS2 exhibited broad-spectrum binding activity against 12 strains, including all tested subvariants. A collaborative study conducted by seven laboratories demonstrated improved harmonization inter-laboratory variability (pre-standardization geometric coefficients variance, 14-314%; post-standardization, 3-35%). ensures an accurate Thus, established national evaluating SARS-CoV-2-specific antibodies (Lot: 300052-202401, 1000 U/mL). Our work provides benchmark vaccines inspires new avenues developing other vaccines.

Language: Английский

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