The role of NOP58 in prostate cancer progression through SUMOylation regulation and drug response DOI Creative Commons
Wei Guo, Shi Zong, Tao Liu

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 18, 2024

Background Prostate cancer is one of the leading causes cancer-related deaths in men. Its molecular pathogenesis closely linked to various genetic and epigenetic alterations, including posttranslational modifications like SUMOylation. Identifying biomarkers that predict outcomes specific therapeutic targets depends on a comprehensive understanding these processes. With growing interest SUMOylation as mechanism affecting prostate genes, this study aimed investigate central role prognostics, focusing significance NOP58. Methods We conducted bioinformatics analysis, integrating differential expression survival gene set enrichment analysis (GSEA), single-cell transcriptomic analyses using data from The Cancer Genome Atlas (TCGA). Key genes were identified through intersections Venn diagrams, Boralta algorithm signatures, machine learning models. These signaling mechanisms validated experimental studies, immunohistochemical staining ontology analyses. Results dual-gene subtype with SUMO1, SUMO2, XPO1 revealed significant differences across subtypes, further emphasizing potential impact NOP58 SUMOylation, key post-translational modification, cancer. overexpression was strongly associated shorter overall (OS), progression-free interval (PFI), disease-specific death patients. Immunohistochemical confirmed significantly overexpressed tissues compared normal tissues. ROC curve demonstrated could distinguish control samples high diagnostic accuracy. Gene Ontology along GSVA GSEA, suggested may be involved cell cycle regulation DNA repair pathways. Moreover, knockdown led increased BCL2 decreased Ki67 levels, promoting apoptosis inhibiting proliferation. Colony formation assays showed inhibited, while its promoted, colony formation, highlighting critical growth survival. Additionally, drug responses, Methotrexate, Rapamycin, Sorafenib, Vorinostat. Conclusion regulator progression mediation pathway. level serves reliable prognostic biomarker an actionable target, advancing precision medicine for Targeting enhance efficacy improve oncology.

Language: Английский

Huc-MSC-derived exosomes alleviates alcohol-induced osteonecrosis of the femoral head through targeting the miR-25-3p/GREM1 axis DOI Creative Commons

Zhifang Tang,

Xiaoyan Xu, Weisong Shi

et al.

Genomics, Journal Year: 2025, Volume and Issue: 117(2), P. 110996 - 110996

Published: Jan. 16, 2025

Language: Английский

Citations

1
Emerging role of sphingolipids and extracellular vesicles in development and therapeutics of cardiovascular diseases DOI Creative Commons

Owais Mohmad Bhat,

Rakeeb Ahmad Mir,

Iqra Bashir Nehvi

et al.

IJC Heart & Vasculature, Journal Year: 2024, Volume and Issue: 53, P. 101469 - 101469

Published: July 23, 2024

Sphingolipids are eighteen carbon alcohol lipids synthesized from non-sphingolipid precursors in the endoplasmic reticulum (ER). The sphingolipids serve as for a vast range of moieties found our cells that play critical role various cellular processes, including cell division, senescence, migration, differentiation, apoptosis, pyroptosis, autophagy, nutrition intake, metabolism, and protein synthesis. In CVDs, different subclasses other derived molecules such sphingomyelin (SM), ceramides (CERs), sphingosine-1-phosphate (S1P) directly related to diabetic cardiomyopathy, dilated myocarditis, ischemic heart disease (IHD), hypertension, atherogenesis. Several genome-wide association studies showed an between genetic variations sphingolipid pathway genes risk CVDs. plays important biogenesis secretion exosomes. Small extracellular vesicles (sEVs)/ exosomes have recently been possible indicators onset linking signaling pathways contribute progression. Important features EVs like biocompatibility, crossing biological barriers can improve pharmacokinetics drugs will be exploited develop next-generation drug delivery systems. this review, we comprehensively discussed sphingolipids, metabolites development addition, concise deliberations were laid discuss sEVs/exosomes regulating pathophysiological processes CVDs therapeutic targets.

Language: Английский

Citations

4
Non-coding RNAs in myocardial ischemia/reperfusion injury and repair DOI
Mingming Pan, Zhixin Li, Xiaohong Wang

et al.

Current Opinion in Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 100825 - 100825

Published: March 1, 2025

Language: Английский

Citations

0
Stem-Cell Derived Exosomal microRNAs as Biomarkers and Therapeutics for Pediatric Cardiovascular Disease DOI Creative Commons
Aaron H. Wasserman,

Bana Abolibdeh,

Reema Hamdan

et al.

Current Treatment Options in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 27(1)

Published: April 9, 2025

In recent years, several pre-clinical studies have demonstrated the therapeutic potential of stem cell-derived exosomes in treatment cardiovascular disease (CVD). Here, we evaluate their as biomarkers for detection and monitoring CVD, with a particular focus on pediatric heart disease. Exosomes isolated from cell sources, including mesenchymal cells (MSCs) pluripotent (PSCs), benefit function, inflammatory responses, angiogenesis injured diseased hearts. These carry variety cargo, such proteins, lipids, nucleic acids. However, majority contain non-coding RNA molecules. Review existing literature RNAs relationship to CVD suggests that containing microRNAs (miRNAs) can serve promising due presence circulation, ease isolation, potential. are especially screening diagnostic tools early congenital

Language: Английский

Citations

0
Exosome-Loaded Tannic Acid–Thioctic Acid Hydrogel Enhances Wound Healing in Coagulation Disorders DOI Creative Commons

Yuesheng Tu,

Weixin Zheng,

Zhenyu Ding

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101496 - 101496

Published: Jan. 25, 2025

Language: Английский

Citations

0
Inflammatory Cell-Targeted Delivery Systems for Myocardial Infarction Treatment DOI Creative Commons
Wenyuan Zhang, Dan Peng, Shiqi Cheng

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(2), P. 205 - 205

Published: Feb. 19, 2025

Myocardial infarction (MI) is a cardiovascular disease (CVD) with high morbidity and mortality worldwide, which serious threat to human life health. Inflammatory immune responses are initiated immediately after MI, unbalanced inflammation post-MI can lead cardiac dysfunction, scarring, ventricular remodeling, emphasizing the critical need for an effective inflammation-regulating treatment. With development of novel therapies, drug delivery system specific inflammatory cells offers significant potential. In this review, we introduce fibroblasts involved in MI summarize newly developed systems related use injectable hydrogels, patches, nanoparticles, extracellular vesicles (EVs). Finally, highlight recent trends cell-targeting involving different strategies that facilitate treatment MI.

Language: Английский

Citations

0
M2 microglia-derived small extracellular vesicles modulate NSC fate after ischemic stroke via miR-25-3p/miR-93-5p-TGFBR/PTEN/FOXO3 axis DOI Creative Commons
Qian Zhang,

Yan Yi,

Tiange Chen

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 23, 2025

Endogenous neurogenesis could promote stroke recovery. Furthermore, anti-inflammatory phenotypical microglia (M2-microglia) facilitate Neural Stem Cell (NSC)-mediated following Ischemic Stroke (IS). Nonetheless, the mechanisms through which M2 influence NSC-mediated post-IS remain unclear. On other hand, microglia-derived small Extracellular Vesicles (M2-sEVs) exert phenomenal biological effects and play significant roles in cell-to-cell interactions, highlighting their potential involvement post-IS, forming basis of this study. M2-sEVs were first isolated from IL-4-stimulated microglia. For vivo tests, intravenously injected into mice every day for 14 days after transient Middle Cerebral Artery Occlusion (tMCAO). Following that, infarct volume neurological function, as well NSC proliferation Subventricular Zone dentate gyrus, migration, differentiation area, examined. vitro administered to subjected Oxygen-Glucose Deprivation (OGD) then reoxygenation, assessed. Finally, microRNA sequencing explore regulatory mechanisms. Our findings revealed that reduced increased score post-tMCAO. M2-sEV treatment promoted neuronal both vitro. Additionally, miR-93-5p miR-25-3p enrichment M2-sEVs. Inhibitors these miRNAs prevented TGFBR, PTEN, FOXO3 downregulation NSC, reversing M2-sEVs' beneficial on sensorimotor differentiation, protected against IS, at least partially, via delivering downregulate expression NSC.

Language: Английский

Citations

0
Immune in myocardial ischemia/reperfusion injury: potential mechanisms and therapeutic strategies DOI Creative Commons

Xiaoyu Xuan,

Jilin Fan, Jingyi Zhang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 8, 2025

Myocardial infarction (MI), which is characterized by high morbidity and mortality, a serious threat to human life health, timely reperfusion therapy save ischemic myocardium currently the most effective intervention. Although effectively restores coronary blood flow maximally limits infarct size, it triggers additional cell death tissue damage, known as myocardial ischemia/reperfusion injury (MIRI). Multiple immune cells are present in area, executing specific functions engaging crosstalk during diverse stages, constituting complex microenvironment involved repair regeneration after MIRI. Immunotherapy brings new hope for treating heart disease modulating microenvironment. In this paper, we explore regulatory roles of various MIRI close relationship between different deaths addition, current status research on targeting system intervene MIRI, with expectation providing basis achieving clinical translation.

Language: Английский

Citations

0
Exosomes and Macrophages: Bidirectional Mutual Regulation in the Treatment of Diabetic Complications DOI
Xue Li,

Lianrong Yang,

Shujun Xu

et al.

Cellular and Molecular Bioengineering, Journal Year: 2024, Volume and Issue: 17(4), P. 243 - 261

Published: Aug. 1, 2024

Language: Английский

Citations

0
The immune regulatory role of exosomal miRNAs and their clinical application potential in heart failure DOI Creative Commons
Dandan Guo, Jianjun Yan, Zhenyu Yang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 2, 2024

Heart failure (HF) is a complex and debilitating condition characterized by the heart's inability to pump blood effectively, leading significant morbidity mortality. The abnormality of immune response key factor in progression HF, contributing adverse cardiac remodeling dysfunction. Exosomal microRNAs (miRNAs) play pivotal role regulating gene expression cellular function, which are integral crosstalk between cells, influencing cell functions, such as macrophage polarization, T activity, cytokine production, thereby modulating various pathological processes inflammation, fibrosis, This review emphasizes immune-regulatory exosomal miRNAs HF highlights their clinical potential diagnostic biomarkers therapeutic agents.

Language: Английский

Citations

0