Homeostatic Control of Deep Sleep inDrosophila: Implications for Discovering Correlates of Sleep Pressure DOI Creative Commons
Budhaditya Chowdhury, Lakshman Abhilash, Antonio Ortega

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Oct. 2, 2022

Abstract Homeostatic control of sleep is typically addressed through mechanical stimulation-induced forced wakefulness and the measurement subsequent increases in sleep. A major confound attends this approach: biological responses to deprivation may reflect a direct response insult rather than loss Similar confounds accompany all forms represent challenge field. Here we describe new paradigm for Drosophila that fully accounts sleep-independent effects. Our results reveal deep states are primary target homeostatic establish presence multi-cycle rebound following deprivation. Furthermore, specific state state-specific rebound. Finally, by accounting molecular effects stimulation during experiments, show serotonin levels track pressure fly’s central brain. illustrate critical need when examining correlates call reassessment work has not accounted such non-specific

Language: Английский

Homeostatic Control of Deep Sleep inDrosophila: Implications for Discovering Correlates of Sleep Pressure DOI Creative Commons
Budhaditya Chowdhury, Lakshman Abhilash, Antonio Ortega

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Oct. 2, 2022

Abstract Homeostatic control of sleep is typically addressed through mechanical stimulation-induced forced wakefulness and the measurement subsequent increases in sleep. A major confound attends this approach: biological responses to deprivation may reflect a direct response insult rather than loss Similar confounds accompany all forms represent challenge field. Here we describe new paradigm for Drosophila that fully accounts sleep-independent effects. Our results reveal deep states are primary target homeostatic establish presence multi-cycle rebound following deprivation. Furthermore, specific state state-specific rebound. Finally, by accounting molecular effects stimulation during experiments, show serotonin levels track pressure fly’s central brain. illustrate critical need when examining correlates call reassessment work has not accounted such non-specific

Language: Английский

Citations

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