Defining spatial nonuniformities of all ipRGC types using an improved Opn4 recombinase mouse line

Cell Reports Methods, Год журнала: 2024, Номер 4(8), С. 100837 - 100837

Опубликована: Авг. 1, 2024

Intrinsically photosensitive retinal ganglion cells (ipRGCs) play a crucial role in several physiological light responses. In this study, we generate an improved Opn4cre knockin allele (Opn4cre(DSO)), which faithfully reproduces endogenous Opn4 expression and improves compatibility with widely used reporters. We evaluated the efficacy sensitivity of Opn4cre(DSO) for labeling retina brain provide in-depth comparison extensively utilized Opn4cre(Saha) line. Through characterization, demonstrated higher specificity ipRGCs minimal recombination escape. Leveraging combination electrophysiological, molecular, morphological analyses, confirmed its detecting all ipRGC types (M1–M6) defined their unique topographical distribution across retina. brain, line labels projections cell bodies. Overall, mouse represents tool studying function distribution, offering means to selectively target these study light-regulated behaviors physiology.

Язык: Английский

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Endogenous opioid signaling in the retina modulates sleep/wake activity in mice

Neurobiology of Sleep and Circadian Rhythms, Год журнала: 2022, Номер 13, С. 100078 - 100078

Опубликована: Июнь 26, 2022

Circadian sleep/wake rhythms are synchronized to environmental light/dark cycles in a process known as photoentrainment. We have previously shown that activation of β-endorphin-preferring μ-opioid receptors (MORs) inhibits the light-evoked firing intrinsically photosensitive retinal ganglion cells (ipRGCs), sole conduits Although we β-endorphin is expressed adult mouse retina, conditions under which unknown. Moreover, it unclear whether endogenous MORs by ipRGCs modulates photoentrainment cycles. To elucidate this, first measured mRNA expression β-endorphin's precursor, proopiomelanocortin (POMC), at various times day quantitative reverse-transcription PCR. POMC appears cyclic retina. then studied with immunohistochemistry and found more highly dark/at night. Finally, used telemetry measure activity, EEG EMG freely moving animals compare wild-type transgenic mice only lack functional MORs. Results from these experiments suggest contribute induction maintenance activity dark phase nocturnal mice, via promotion wakefulness inhibition slow-wave sleep. Together, data activates modulate pathway.

Язык: Английский

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Melanopic Limits of Metamer Spectral Optimisation in Multi-Channel Smart Lighting Systems

Energies, Год журнала: 2021, Номер 14(3), С. 527 - 527

Опубликована: Янв. 20, 2021

Modern indoor lighting faces the challenge of finding an appropriate balance between energy consumption, legal requirements, visual performance, and circadian effectiveness a spectrum. Multi-channel LED luminaires have option keeping image-forming metrics steady while varying melanopic radiance through metamer spectra for non-visual purposes. Here, we propose theoretical concept automated smart system that is designed to satisfy user’s preference neural networks triggering pathway via metamers. To quantify limits metamers at chromaticity point, used 561 coordinates along Planckian locus (2700 K 7443 K, ±Duv 0 0.048) as optimisation targets generated by using 6-channel, 8-channel, 11-channel combination three different luminance levels. We found in best-case scenario, can be varied up 65% constant (Δu′v′≤7.05×10−5) spectra. The highest contrast reached near 3292 4717 within Duv range −0.009 0.006. Additionally, publish over 1.2 million optimised multichannel open-source dataset with this work.

Язык: Английский

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Intrinsic circadian timekeeping properties of the thalamic lateral geniculate nucleus

Journal of Neuroscience Research, Год журнала: 2021, Номер 99(12), С. 3306 - 3324

Опубликована: Ноя. 10, 2021

Abstract Circadian rhythmicity in mammals is sustained by the central brain clock—the suprachiasmatic nucleus of hypothalamus (SCN), entrained to ambient light–dark conditions through a dense retinal input. However, recent discoveries autonomous clock gene expression cast doubt on supremacy SCN and suggest circadian timekeeping mechanisms devolve local clocks. Here, we use combination molecular, electrophysiological, optogenetic tools evaluate intrinsic properties main retinorecipient thalamic center—the lateral geniculate (LGN) male rats mice. We identify dorsolateral as slave oscillator, which exhibits core exclusively vivo . Additionally, provide compelling evidence for accompanied variation neuronal activity intergeniculate leaflet ventrolateral (VLG). Finally, our experiments propose VLG light‐entrainable whose phase may be advanced input at beginning projected night. Altogether, this study first time demonstrates shaping physiology LGN.

Язык: Английский

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Immediate responses to ambient light in vivo reveal distinct subpopulations of suprachiasmatic VIP neurons

iScience, Год журнала: 2023, Номер 26(10), С. 107865 - 107865

Опубликована: Сен. 9, 2023

The circadian rhythm pacemaker, the suprachiasmatic nucleus (SCN), mediates light entrainment via vasoactive intestinal peptide (VIP) neurons (SCNVIP). Yet, how these uniquely respond and connect to intrinsically photosensitive retinal ganglion cells (ipRGCs) expressing melanopsin (Opn4) has not been determined functionally in freely behaving animals. To address this, we first used monosynaptic tracing from SCNVIP mice identified two subpopulations. Second, recorded calcium changes response ambient light, at both bulk single-cell levels, found unique activity patterns high- low-intensity blue light. of subpopulations could be manipulated by application an Opn4 antagonist. These results suggest that types Opn4-expressing ipRGCs, likely M1 M2, but only one is responsive red findings have important implications for our basic understanding non-image-forming processing.

Язык: Английский

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