Mechanism of desuccinylation of G6PD mediated by SIRT7 to promote vitiligo disease progression DOI Creative Commons

Yiyun Yang,

Haidong Long,

Long Lan

и другие.

Immunity Inflammation and Disease, Год журнала: 2024, Номер 12(8)

Опубликована: Авг. 1, 2024

Abstract Background Sirtuin 7 (SIRT7) is pivotal in diverse diseases progression. Importantly, SIRT7 associated with melanin production. However, whether regulates vitiligo unclear. Therefore, we aimed to investigate the effects of on pigmentation and modification glucose 6‐phosphate dehydrogenase (G6PD). Methods After knockdown G6PD, melanocytes was evaluated using commercial kits, immunofluorescence, Western blot analysis. The succinylation G6PD mediated by analyzed co‐immunoprecipitation, analysis, cycloheximide‐chase experiment. Results We found that highly expressed skin lesions. Knockdown increased tyrosinase activity, content, levels α‐melanocyte‐stimulating hormone, MITF, TYR, TRP1, TRP2. Additionally, directly interacted G6PD. Silenced promoted enhanced its protein stability. reversed effect reduced expression Conclsuion Silencing promotes succinylating suggesting SIRT7‐mediated desuccinylation may promote

Язык: Английский

Unraveling the multifaceted role of SIRT7 and its therapeutic potential in human diseases DOI
Li Han,

Ziyue Yuan,

Junhao Wu

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 279, С. 135210 - 135210

Опубликована: Авг. 30, 2024

Язык: Английский

Процитировано

4
Mechanism of desuccinylation of G6PD mediated by SIRT7 to promote vitiligo disease progression DOI Creative Commons

Yiyun Yang,

Haidong Long,

Long Lan

и другие.

Immunity Inflammation and Disease, Год журнала: 2024, Номер 12(8)

Опубликована: Авг. 1, 2024

Abstract Background Sirtuin 7 (SIRT7) is pivotal in diverse diseases progression. Importantly, SIRT7 associated with melanin production. However, whether regulates vitiligo unclear. Therefore, we aimed to investigate the effects of on pigmentation and modification glucose 6‐phosphate dehydrogenase (G6PD). Methods After knockdown G6PD, melanocytes was evaluated using commercial kits, immunofluorescence, Western blot analysis. The succinylation G6PD mediated by analyzed co‐immunoprecipitation, analysis, cycloheximide‐chase experiment. Results We found that highly expressed skin lesions. Knockdown increased tyrosinase activity, content, levels α‐melanocyte‐stimulating hormone, MITF, TYR, TRP1, TRP2. Additionally, directly interacted G6PD. Silenced promoted enhanced its protein stability. reversed effect reduced expression Conclsuion Silencing promotes succinylating suggesting SIRT7‐mediated desuccinylation may promote

Язык: Английский

Процитировано

2