Cell Painting: a decade of discovery and innovation in cellular imaging
Nature Methods,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 5, 2024
Язык: Английский
Flexible and stretchable bioelectronics for organoids
Med-X,
Год журнала:
2025,
Номер
3(1)
Опубликована: Фев. 1, 2025
Abstract
Organoids
have
gained
significant
interest
due
to
their
ability
recapitulate
the
structural,
molecular,
and
functional
complexity
of
corresponding
organs.
While
methods
been
developed
characterize
benchmark
organoid
structural
molecular
properties,
capturing
development
maturation
organoids
remains
challenging.
To
address
this,
multifunctional
bioelectronics
for
interfacing
with
has
actively
pursued.
However,
conventional
electronics
face
limitations
in
achieving
recording
control
across
entire
three-dimensional
(3D)
volume
a
long-term
stable
manner
large
morphological
cellular
composition
changes
during
development.
In
this
review,
we
first
discuss
application
interfacing.
We
then
focus
on
flexible
stretchable
designed
create
organoid/electronics
hybrids
chronically
interfaces.
also
review
recent
advancements
charting
multimodal
cell
activities
throughout
Furthermore,
explore
integration
other
characterization
modalities
comprehensive
cells
within
3D
tissues.
Finally,
potential
integrating
artificial
intelligence
into
system
through
embedded
electronics,
harnessing
biosymbiotic
computational
systems.
These
could
provide
valuable
tools
characterizing
maturation,
establishing
patient-specific
models,
developing
therapeutic
opportunities,
exploring
novel
strategies.
Graphical
abstract
Язык: Английский
HIPPIE: A Multimodal Deep Learning Model for Electrophysiological Classification of Neurons
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 15, 2025
Abstract
Extracellular
electrophysiological
recordings
present
unique
computational
challenges
for
neuronal
classification
due
to
noise,
technical
variability,
and
batch
effects
across
experimental
systems.
We
introduce
HIPPIE
(High-dimensional
Interpretation
of
Physiological
Patterns
In
recordings),
a
deep
learning
framework
that
combines
self-supervised
pretraining
on
unlabeled
datasets
with
supervised
fine-tuning
classify
neurons
from
extracellular
recordings.
Using
conditional
convolutional
joint
autoencoders,
learns
robust,
technology-adjusted
representations
waveforms
spiking
dynamics.
This
model
can
be
applied
clustering
diverse
biological
cultures
technologies.
validated
both
in
vivo
mouse
vitro
brain
slices,
where
it
demonstrated
superior
performance
over
other
unsupervised
methods
cell-type
discrimination
aligned
closely
anatomically
defined
classes.
Its
latent
space
organizes
along
gradients,
while
enabling
individual
corrected
alignment
experiments.
establishes
general
systematically
decoding
diversity
native
engineered
Язык: Английский
Special Mini-Issue: Quantitative methods to decipher cellular heterogeneity – from single-cell to spatial omic methods
Biophysical Reviews,
Год журнала:
2024,
Номер
16(1), С. 11 - 12
Опубликована: Янв. 30, 2024
Язык: Английский
Biophysical Reviews: peering into 2024
Biophysical Reviews,
Год журнала:
2024,
Номер
16(1), С. 1 - 7
Опубликована: Фев. 1, 2024
Язык: Английский
Integration of hyperspectral imaging and transcriptomics from individual cells with HyperSeq
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 30, 2024
Abstract
Microscopy
and
omics
are
complementary
approaches
to
probe
the
molecular
state
of
cells
in
health
disease,
combining
granularity
with
scalability.
While
important
advances
have
been
achieved
over
last
decade
each
area,
integrating
both
imaging-
sequencing-based
assays
on
same
cell
has
proven
challenging.
In
this
study,
a
new
approach
called
HyperSeq
that
combines
hyperspectral
autofluorescence
imaging
transcriptomics
is
demonstrated.
was
applied
Michigan
Cancer
Foundation
7
(MCF-7)
breast
cancer
identified
subpopulation
exhibiting
bright
rings
at
plasma
membrane
optical
channel
13
(
λ
ex
=
431
nm,
em
594
nm).
Correlating
presence
ring
gene
expression
indicated
ringed
more
likely
express
hallmark
genes
apoptosis
less
associated
ATP
production.
Further,
correlation
morphology
suggested
multiple
members
spliceosome
were
downregulated
larger
MCF-7
cells.
Multiple
evenly
expressed
across
sizes
but
also
exhibited
higher
usage
specific
exons
or
smaller
Finally,
between
fluorescence
within
spectral
range
Nicotinamide
adenine
dinucleotide
hydrogen
(NADH)
provided
insight
into
metabolic
states
These
observations
link
cell’s
spectrum
its
internal
state,
demonstrating
utility
study
biology
single
resolution
by
spectral,
morphological
transcriptomic
analyses
single,
streamlined
workflow.
Язык: Английский
Gene Expression, Morphology, and Electrophysiology During the Dynamic Development of Human Induced Pluripotent Stem Cell-Derived Atrial- and Ventricular-Like Cardiomyocytes [Letter]
Biologics,
Год журнала:
2024,
Номер
Volume 18, С. 145 - 146
Опубликована: Июнь 1, 2024
Язык: Английский