Model‐Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production DOI Creative Commons
Zeynep Efsun Duman‐Özdamar, Mattijs K. Julsing, Vitor A. P. Martins dos Santos

и другие.

Microbial Biotechnology, Год журнала: 2025, Номер 18(3)

Опубликована: Март 1, 2025

Extensive usage of plant-based oils, especially palm oil, has led to environmental and social issues, such as deforestation loss biodiversity, thus sustainable alternatives are required. Microbial from Yarrowia lipolytica, offer a promising solution because their similar composition low carbon footprint ability utilise low-cost substrates. In this study, we employed the Design-Build-Test-Learn (DBTL) approach enhance lipid production in Y. lipolytica. We systematically evaluated predictions genome-scale metabolic model identify overcome bottlenecks biosynthesis. tested effect predicted medium supplements (glutamate, leucine, methionine threonine) genetic intervention targets, including overexpression ATP-citrate lyase (ACL), acetyl-CoA carboxylase (ACC), threonine synthase (TS), diacylglycerol acyltransferase(DGA1), deletion citrate exporter gene (CEX1) disruption β-oxidation pathway (MFE1). This work revealed critical roles ACC, ACL, TS DGA1 interaction these genes with elevated intracellular availability Combining Δmfe_Δcex background achieved remarkable 200% increase content (56% w/w) 230% yield on glycerol. These findings underscore potential lipolytica an efficient microbial cell factory for fatty acid production. Our study advances understanding metabolism demonstrates viable developing economically feasible oil.

Язык: Английский

Model‐Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production DOI Creative Commons
Zeynep Efsun Duman‐Özdamar, Mattijs K. Julsing, Vitor A. P. Martins dos Santos

и другие.

Microbial Biotechnology, Год журнала: 2025, Номер 18(3)

Опубликована: Март 1, 2025

Extensive usage of plant-based oils, especially palm oil, has led to environmental and social issues, such as deforestation loss biodiversity, thus sustainable alternatives are required. Microbial from Yarrowia lipolytica, offer a promising solution because their similar composition low carbon footprint ability utilise low-cost substrates. In this study, we employed the Design-Build-Test-Learn (DBTL) approach enhance lipid production in Y. lipolytica. We systematically evaluated predictions genome-scale metabolic model identify overcome bottlenecks biosynthesis. tested effect predicted medium supplements (glutamate, leucine, methionine threonine) genetic intervention targets, including overexpression ATP-citrate lyase (ACL), acetyl-CoA carboxylase (ACC), threonine synthase (TS), diacylglycerol acyltransferase(DGA1), deletion citrate exporter gene (CEX1) disruption β-oxidation pathway (MFE1). This work revealed critical roles ACC, ACL, TS DGA1 interaction these genes with elevated intracellular availability Combining Δmfe_Δcex background achieved remarkable 200% increase content (56% w/w) 230% yield on glycerol. These findings underscore potential lipolytica an efficient microbial cell factory for fatty acid production. Our study advances understanding metabolism demonstrates viable developing economically feasible oil.

Язык: Английский

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