Nature-Inspired Strategies for Sustainable Degradation of Synthetic Plastics

JACS Au, Год журнала: 2024, Номер 4(9), С. 3323 - 3339

Опубликована: Авг. 27, 2024

Synthetic plastics have become integral to our daily lives, yet their escalating production, limited biodegradability, and inadequate waste management contribute environmental contamination. Biological plastic degradation is one promising strategy address this pollution. The inherent chemical physical properties of synthetic plastics, however, pose challenges for microbial enzymes, hindering the effective development a sustainable biological recycling process. This Perspective explores alternative, nature-inspired strategies designed overcome some key limitations in currently available plastic-degrading enzymes. Nature's refined pathways natural polymers, such as cellulose, present compelling framework efficient technologies enzymatic degradation. By drawing insights from nature, we propose general employing substrate binding domains improve targeting multienzyme scaffolds efficiency limitations. As potential application, outline pathway upcycle polyethylene into alkenes. Employing can path toward solution impact plastics.

Язык: Английский

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Targeted Genome Mining Facilitates the Discovery of a Promiscuous, Hyperthermostable Amidase from Thermovenabulum gondwanense with Notable Nylon‐Degrading Capacity

Angewandte Chemie, Год журнала: 2024, Номер unknown

Опубликована: Окт. 23, 2024

Abstract Plastics are ubiquitous in our ecosystems, and microplastic accumulation the environment is an emerging global health concern. Since available recycling technologies not economically competitive with primary plastic production, use expected to reach 1231 megatons by 2060, 493 leeching into each year. To identify new nylon‐recycling biotechnologies, targeted genome mining was used thermostable enzymes capable of degrading polyamides. Here, we describe characterization a novel protein sourced from Thermovenabulum gondwanense : TvgC. TvgC extremely stable, exhibiting melting temperature 93 °C no detectable losses hydrolytic activity after one week at 60 °C. While nylonases primarily process nylon‐6, catalysed degradation both nylon‐6 nylon‐6,6 films, which considerably more difficult degrade. Finally, conversion experiments demonstrate that achieves 1.2 wt % film, comparable most highly engineered nylonases. This hyperthermostable represents excellent starting point for future engineering increasingly efficient

Язык: Английский

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Characterization of Potential Plastic-Degradation Enzymes from Marine Bacteria

ACS Omega, Год журнала: 2024, Номер 9(29), С. 32185 - 32192

Опубликована: Июль 8, 2024

Polyethylene terephthalate (PET) and polyethylene (PE) are prominent polymer materials that comprise a significant portion of commercial plastic waste. Their durability slow degradation rate have resulted in accumulation on Earth. In recent study, macrotranscriptomic profiling reconstituted marine bacterial community identified 10 putative enzymes capable directly acting PE or PET (PEases PETases). Among these enzymes, three recombinant proteins were reported to possess activity. To select potential degrading enzyme candidates for protein engineering efforts, we expressed purified eight out the candidates, excluding two due poor expression and/or solubility. Notably, several candidate displayed esterase activity p-nitrophenyl butyrate exhibited unexpected thermostability despite their origin. Additionally, observed dose- time-dependent hydrolytic trimer substrate. Structural analysis mutagenesis confirmed presence catalytic triad residues, classifying it as an esterase. Furthermore, elucidated structural importance disulfide bonds. Through point mutation experiments, enhanced selected nanoparticles. Our findings challenge classification highlight significance complexity validating through metagenomic analysis.

Язык: Английский

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Targeted Genome Mining Facilitates the Discovery of a Promiscuous, Hyperthermostable Amidase from Thermovenabulum gondwanense with Notable Nylon‐Degrading Capacity

Angewandte Chemie International Edition, Год журнала: 2024, Номер unknown

Опубликована: Окт. 23, 2024

Abstract Plastics are ubiquitous in our ecosystems, and microplastic accumulation the environment is an emerging global health concern. Since available recycling technologies not economically competitive with primary plastic production, use expected to reach 1231 megatons by 2060, 493 leeching into each year. To identify new nylon‐recycling biotechnologies, targeted genome mining was used thermostable enzymes capable of degrading polyamides. Here, we describe characterization a novel protein sourced from Thermovenabulum gondwanense : TvgC. TvgC extremely stable, exhibiting melting temperature 93 °C no detectable losses hydrolytic activity after one week at 60 °C. While nylonases primarily process nylon‐6, catalysed degradation both nylon‐6 nylon‐6,6 films, which considerably more difficult degrade. Finally, conversion experiments demonstrate that achieves 1.2 wt % film, comparable most highly engineered nylonases. This hyperthermostable represents excellent starting point for future engineering increasingly efficient

Язык: Английский

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Chemical Reactivity of Weathered Nanoplastics and Their Interactions with Heavy Metals

Environmental Science Nano, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Nanoplastics (NPs) are chemically reactive following abiotic and biotic weathering processes. These weathered NPs have the potential to facilitate transformation of legacy contaminants, such as heavy metals.

Язык: Английский

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