Molecular Aspects of Medicine, Год журнала: 2022, Номер 90, С. 101147 - 101147
Опубликована: Окт. 13, 2022
Язык: Английский
Carbohydrate Polymers, Год журнала: 2024, Номер 343, С. 122471 - 122471
Опубликована: Июль 9, 2024
Язык: Английский
Процитировано
5
Molecular Aspects of Medicine, Год журнала: 2022, Номер 90, С. 101141 - 101141
Опубликована: Сен. 9, 2022
Язык: Английский
Процитировано
16
Frontiers in Immunology, Год журнала: 2025, Номер 16
Опубликована: Май 13, 2025
Tumors may utilize different strategies to escape T cell immunosurveillance. Besides the overexpression of checkpoint ligands (such as PDL1) or secretion immunosuppressive agents, several studies have shown that cancer aberrant sialylation can, through interaction with selected receptors such those from Siglec family, neutralize NK and function. Herein, we wanted take advantage presence inhibitory sialic acid on tumor surface enhance anti-tumor activity. To this end, devised a novel chimeric receptor consisting extracellular portion Siglec-7 intracellular 41BB, which can convert signals into stimulatory ones when expressed in human T-cells. This co-stimulatory switch (CSR), co-expressed tumor-specific TCR, facilitated higher cytokine activation profiles following co-culture cells. Additionally, cells equipped CSR demonstrated improved function vivo. Given broad expression pattern cells, our data suggest act general adjuvant boost TCR Overall, work provides an approach improve engineered T-cell-based treatment.
Язык: Английский
Процитировано
0
Cell Stress and Chaperones, Год журнала: 2025, Номер unknown, С. 100083 - 100083
Опубликована: Май 1, 2025
Innate immune responses to cell damage-associated molecular patterns induce a controlled degree of inflammation, ideally avoiding the promotion intense unwanted inflammatory adverse events. When released by damaged cells, Hsp70 can stimulate different that range from activation suppression. The effects are mediated through innate receptors expressed primarily myeloid such as dendritic cells (DCs). regulatory bind extracellular mouse (mHsp70) not fully characterized, and neither their potential interactions with activating receptors. Here, we show mHsp70 interacts receptor complex formed both inhibitory Siglec-E LOX-1 on DCs. We also find this interaction takes place in lipid microdomains within plasma membrane, acts negative regulator LOX-1-mediated upon or oxidized LDL binding. Thus, modulate surface. These findings add another dimension mechanism indicate how self-molecules contribute dampening exacerbated responses.
Язык: Английский
Процитировано
0
Current Opinion in Chemical Biology, Год журнала: 2024, Номер 81, С. 102502 - 102502
Опубликована: Июль 18, 2024
Aberrant Siglec expression in the tumour microenvironment has been implicated malignancies and can impact behaviour patient survival. Further to this, engagement with sialoglycans induces masked antigen recognition promotes immune evasion, highlighting deregulated function. This necessitates elucidation of their profiles progression. MicroRNAs (miRNAs) mediated targeting represents a novel approach further elucidate potential clinical relevance. Although miRNA activity remains limited, we highlight current literature detailing miRNA:Siglec interactions within landscape provide insights for possible diagnostic therapeutic strategies Siglec/sialic acid axis.
Язык: Английский
Процитировано
3
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(6), С. 5512 - 5512
Опубликована: Март 14, 2023
Since the role of sialome-Siglec axis has been described as a regulatory checkpoint immune homeostasis, promotion stimulatory or inhibitory Siglec-related mechanisms is crucial in cancer progression and therapy. Here, we investigated effect tamoxifen on sialic acid-Siglec interplay its significance conversion breast cancer. To mimic tumour microenvironment, used oestrogen-dependent oestrogen-independent cells/THP-1 monocytes transwell co-cultures exposed to and/or β-estradiol. We found changes cytokine profiles accompanied by phenotype switching, measured expression arginase-1. The immunomodulatory effects THP-1 cells occurred with altered SIGLEC5 SIGLEC14 genes their products, confirmed RT-PCR flow cytometry. Additionally, exposure increased binding Siglec-5 Siglec-14 fusion proteins cells; however, these appeared be unassociated oestrogen dependency. Our results suggest that tamoxifen-induced alterations activity reflect crosstalk between Siglec-expressing tumour's sialome. Given distribution Siglec-5/14, profile activatory Siglecs patients may useful verification therapeutic strategies predicting behaviour patient's overall survival.
Язык: Английский
Процитировано
6
Heliyon, Год журнала: 2024, Номер 10(2), С. e24286 - e24286
Опубликована: Янв. 1, 2024
Siglecs belong to a family of immune regulatory receptors predominantly found on hematopoietic cells. They interact with Sia, resulting in the activation or inhibition response. Previous reports have suggested that
Язык: Английский
Процитировано
2
Seminars in Immunology, Год журнала: 2024, Номер 74-75, С. 101893 - 101893
Опубликована: Июль 1, 2024
Язык: Английский
Процитировано
2
Seminars in Immunology, Год журнала: 2024, Номер 77, С. 101925 - 101925
Опубликована: Дек. 19, 2024
Язык: Английский
Процитировано
2
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Дек. 4, 2023
Innate immune responses to cell damage-associated molecular patterns induce a controlled degree of inflammation, ideally avoiding the promotion intense unwanted inflammatory adverse events. When released by damaged cells, Hsp70 can stimulate different that range from activation suppression. The effects are mediated through innate receptors expressed primarily myeloid such as dendritic cells (DCs). regulatory bind extracellular mouse (mHsp70) not fully characterized, and neither their potential interactions with activating receptors. Here, we describe mHsp70 interacts receptor complex formed inhibitory Siglec-E LOX-1 on DCs. We also find this interaction takes place within lipid microdomains, acts negative regulator LOX-1-mediated upon or oxidized LDL binding. Thus, HSP70 both modulate surface. These findings add another dimension mechanism how self-molecules contribute dampening exacerbated responses.
Язык: Английский
Процитировано
4