Heme dependent activity of the Streptomyces c-di-GMP-metabolizing enzyme CdgA
Journal of Inorganic Biochemistry,
Год журнала:
2025,
Номер
269, С. 112874 - 112874
Опубликована: Фев. 27, 2025
Streptomyces
species
are
vital
for
producing
natural
products
like
antibiotics,
with
c-di-GMP
playing
a
key
role
in
regulating
processes
such
as
differentiation.
C-di-GMP
metabolism
is
controlled
by
diguanylate
cyclases
(DGCs)
and
phosphodiesterases
(PDEs),
which
synthesize
hydrolyze
c-di-GMP,
respectively,
to
modulate
cellular
levels.
To
improve
our
understanding
of
c-di-GMP-regulated
Streptomyces,
we
have
characterized
c-di-GMP-metabolizing
enzyme
CdgA
from
ghanaensis
that
contains
both
cyclase
phosphodiesterase
domain.
Our
studies
demonstrate
the
purified
form
without
heme
only
able
degrade
c-di-GMP.
When
reconstituted
heme,
it
enables
synthesis,
depending
on
redox
state
synthesis
rate
changed.
knowledge,
this
first
heme-dependent
activity
reported
has
major
implications
way
metabolized
vivo
Streptomyces.
Язык: Английский
Microbe Profile: Streptomyces venezuelae – a model species to study morphology and differentiation in filamentous bacteria
Microbiology,
Год журнала:
2025,
Номер
171(3)
Опубликована: Март 13, 2025
Graphical
Abstract
Streptomyces
venezuelae
undergoing
the
spore-to-spore
life
cycle
in
liquid
culture
makes
it
a
versatile
model
organism
for
generating
key
insights
into
regulatory
networks,
cell
biology
and
development
of
this
genus.
Язык: Английский
The protocatechuic acid catabolism regulator PcaV inhibits lincomycin biosynthesis either directly or indirectly through the control of the expression of other regulatory or metabolic genes
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
311, С. 143649 - 143649
Опубликована: Апрель 29, 2025
Язык: Английский
Impact of the alkaline volatile trimethylamine on the physiology of Streptomyces venezuelae : an integrated transcriptomic and metabolomics study
Microbiology Spectrum,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 27, 2025
ABSTRACT
Streptomyces
bacteria
use
volatile
compounds
for
a
range
of
functions,
including
communication
and
to
kill
competing
species.
We
previously
showed
that
the
wild-type
venezuelae
strain
ISP5230
produces
alkaline
trimethylamine
(TMA),
which
can
completely
rescue
morphological
defects
S.
mutant
MU-1,
suggesting
TMA
have
significant
impact
on
physiology.
In
this
study,
we
further
characterized
MU-1
elucidated
effects
volatiles
by
comparing
transcriptomic
data
MU-1.
Our
RNA-sequence
analysis
revealed
exhibited
differentially
expressed
genes
(DEGs)
in
multiple
categories,
involved
development,
carbon
metabolism,
antibiotic
production,
transport,
these
were
verified
real-time
PCR
analysis.
Using
metabolomics
analysis,
next
organic
acids
accumulated
consistent
with
acidification
growth
medium
addition,
our
quantitative
much
higher
residual
glucose
content
inefficient
consumption
mutant.
Notably,
exposure
restored
normal
expression
pattern
DEGs
ability
phenotype,
morphology,
medium,
uptake,
production;
findings
indicated
global
conclusion,
study
suggests
profoundly
affects
physiology
may
promote
mutants,
enabling
their
survival
complex
microbial
communities.
IMPORTANCE
Microbes
produce
wide
array
diverse
biological
roles
been
implicated
volatiles.
,
(TMA)
enables
between
cells,
could
also
defective
morphology
indicating
broader
identified
demonstrated
induced
levels
comparable
those
strain,
abnormal
phenotype
expands
role
recipient
cells
adds
new
understanding
importance
Язык: Английский
Regulation of daptomycin biosynthesis in Streptomyces roseosporus: new insights from genomic analysis and synthetic biology to accelerate lipopeptide discovery and commercial production
Natural Product Reports,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Complex
regulatory
mechanisms
govern
daptomycin
biosynthesis
in
Streptomyces
roseosporus
.
Ten
genes
modulate
transcription
of
biosynthetic
from
a
single
promoter,
and
translation
is
coordinated
by
translational
coupling.
Язык: Английский
Characterization of glycogen-related glycoside hydrolase glgX and glgB from Klebsiella pneumoniae and their roles in biofilm formation and virulence
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Дек. 18, 2024
Glycogen
is
a
polymer
used
by
bacteria
to
store
excess
glucose,
playing
crucial
role
in
bacterial
growth,
stress
resistance,
biofilm
formation,
and
virulence.
In
bacteria,
the
glycoside
hydrolase
family
13
protein
are
involved
synthesis
metabolism
of
glycogen,
respectively.
The
absence
these
enzymes
leads
changes
glycogen
content,
thereby
affecting
growth
strain.
To
date,
research
on
roles
glycogen-related
genes
phenotypes
Язык: Английский