Chemical Reviews,
Год журнала:
2024,
Номер
124(5), С. 2553 - 2582
Опубликована: Март 4, 2024
The
intricate
and
complex
features
of
enzymatic
reaction
networks
(ERNs)
play
a
key
role
in
the
emergence
sustenance
life.
Constructing
such
vitro
enables
stepwise
build
up
complexity
introduces
opportunity
to
control
activity
using
physicochemical
stimuli.
Rational
design
modulation
network
motifs
enable
engineering
artificial
systems
with
emergent
functionalities.
Such
functional
are
useful
for
variety
reasons
as
creating
new-to-nature
dynamic
materials,
producing
value-added
chemicals,
constructing
metabolic
modules
synthetic
cells,
even
enabling
molecular
computation.
In
this
review,
we
offer
insights
into
chemical
characteristics
ERNs
while
also
delving
their
potential
applications
associated
challenges.
Green Chemistry,
Год журнала:
2023,
Номер
25(18), С. 7041 - 7057
Опубликована: Янв. 1, 2023
Biocatalysts
raised
by
the
green
chemistry
and
circular
principles
can
constitute
most
important
efficient
strategy
for
achieving
many
of
17
Sustainable
Development
Goals
launched
UN.
Molecules,
Год журнала:
2023,
Номер
28(14), С. 5520 - 5520
Опубликована: Июль 19, 2023
Direct
biocatalytic
processes
for
CO2
capture
and
transformation
in
value-added
chemicals
may
be
considered
a
useful
tool
reducing
the
concentration
of
this
greenhouse
gas
atmosphere.
Among
other
enzymes,
carbonic
anhydrase
(CA)
formate
dehydrogenase
(FDH)
are
two
key
biocatalysts
suitable
challenge,
facilitating
uptake
carbon
dioxide
from
atmosphere
complementary
ways.
Carbonic
anhydrases
accelerate
by
promoting
its
solubility
water
form
hydrogen
carbonate
as
first
step
converting
into
species
widely
used
storage
utilization
(CCSU),
particularly
carbonation
mineralization
methods.
On
hand,
dehydrogenases
represent
machinery
evolved
certain
organisms
to
convert
enriched,
reduced,
easily
transportable
species,
such
formic
acid,
via
enzymatic
cascade
systems
that
obtain
energy
chemical
electrochemical
sources,
or
light.
Formic
acid
is
basis
fixing
C1-carbon
other,
more
reduced
molecules.
In
review,
state-of-the-art
both
methods
assessed,
highlighting
biotechnological
approaches
have
been
developed
using
enzymes.
Carbon Resources Conversion,
Год журнала:
2024,
Номер
7(4), С. 100234 - 100234
Опубликована: Март 3, 2024
Hydrogenating
carbon
dioxide
to
formate
using
dehydrogenase
(FDH)
is
a
sustainable
approach
for
CO2
mitigation.
Herein,
we
developed
biocatalytic
system
with
cofactor
regeneration
by
immobilizing
multiple
enzymes,
namely
FDH,
carbonic
anhydrase
(CA),
and
glutamate
(GDH),
on
hydrophobic
surface
modified
MOF,
SA-HKUST-1.
The
adsorption
kinetics
of
the
enzymes
SA-HKUST-1
were
described
pseudo
second-order
model,
while
equilibrium
followed
Freundlich
isotherm.
Formate
production
immobilized
was
3.75
times
higher
than
that
achieved
free
8.4
FDH
alone
interaction
between
support
altered
secondary
structure
retained
94%
their
activity
after
four
reuse
cycles.
This
study
provides
novel
insights
into
combined
effect
catalytic
efficiency
stability
FDH.
These
findings
can
provide
basis
developing
highly
stable
continuous
hydrogenation
at
industrial
level.
Nicotinamide
adenine
dinucleotide
(NADH)
and
nicotinamide
phosphate
(NADPH)
constitute
major
hydrogen
donors
for
oxidative/reductive
bio-transformations.
NAD(P)H
regeneration
systems
coupled
with
formate
dehydrogenases
(FDHs)
represent
a
dreamful
method.
However,
most
of
the
native
FDHs
are
NAD+
-dependent
suffer
from
insufficient
reactivity
compared
to
other
enzymatic
tools,
such
as
glucose
dehydrogenase.
An
efficient
competitive
NADP+
-utilizing
FDH
necessitates
availability
robustness
NADPH
systems.
Herein,
we
report
engineering
new
Candida
dubliniensis
(CdFDH),
which
showed
no
strict
preference
by
structure-guided
rational/semi-rational
design.
A
combinatorial
mutant
CdFDH-M4
(D197Q/Y198R/Q199N/A372S/K371T/▵Q375/K167R/H16L/K159R)
exhibited
75-fold
intensification
catalytic
efficiency
(kcat
/Km
).
Moreover,
has
been
successfully
employed
in
diverse
asymmetric
processes
cofactor
total
turnover
numbers
(TTNs)
ranging
135
986,
making
it
potentially
useful
NADPH-required
biocatalytic
transformations.
Critical Reviews in Food Science and Nutrition,
Год журнала:
2024,
Номер
unknown, С. 1 - 18
Опубликована: Июнь 27, 2024
Anthocyanins
(ACNs)
are
secondary
metabolites
found
in
plants.
Due
to
their
impressive
biological
activities,
ACNs
have
gained
significant
popularity
and
extensive
application
within
the
food,
pharmaceutical,
nutraceutical
industries.
A
derivative
of
ACNs:
pyranoanthocyanins
(PACNs)
possesses
more
stable
properties
interesting
activities.
However,
conventional
methods
for
production
ACNs,
including
chemical
synthesis
plant
extraction,
involve
organic
solvents.
Microbial
from
renewable
biomass,
such
as
amino
acids
or
flavonoids,
is
considered
a
sustainable
environmentally
friendly
method
large-scale
ACNs.
Recently,
construction
microbial
cell
factories
(MCFs)
efficient
biosynthesis
PACNs
has
attracted
much
attention.
In
this
review,
we
summarize
cases
analyze
bottlenecks
reconstructing
metabolic
pathways
synthesizing
microorganisms.
Consequently,
there
an
urgent
need
investigate
mechanisms
behind
development
MCFs
synthesis.
Such
research
also
holds
promise
advancing
food
pigments.
Meanwhile,
propose
potential
solutions
bottleneck
problem
based
on
engineering
enzyme
engineering.
Finally,
prospects
natural
biotechnology
discussed.
