Chemistry - A European Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 10, 2024
Abstract
The
development
of
redox‐sensitive
molecular
fluorescent
probes
for
the
detection
redox
changes
in
Plasmodium
falciparum
‐parasitized
red
blood
cells
remains
interest
due
to
limitations
current
genetically
encoded
biosensors.
This
study
describes
design,
screening
and
synthesis
new
pro‐fluorophores
based
on
flavylium
azido
dyes
coupled
by
CuAAC
click
chemistry
alkynyl
analogues
plasmodione
oxide,
key
metabolite
potent
redox‐active
antimalarial
plasmodione.
photophysical
electrochemical
properties
these
were
evaluated,
focusing
their
fluorogenic
responses.
influence
both
status
quinone
length
PEG
chain
separating
fluorophore
from
electrophore
was
investigated.
fluorescence
quenching
photoinduced
electron
transfer
is
reversible
high
amplitude
oxidized
forms
reinstated
reduced
hydroquinone
forms.
Our
results
demonstrate
that
shortening
has
effect
enhancing
response,
likely
non‐covalent
interactions
between
two
chromophores.
All
systems
evaluated
antiparasitic
activities
imaging
suggests
efficacy
P.
cells,
paving
way
future
parasite
studies
monitor
cellular
processes.
Chemosensors,
Год журнала:
2024,
Номер
12(4), С. 58 - 58
Опубликована: Апрель 8, 2024
Pharmaceutical
opioids
are
intravenously
or
orally
administered
analgesics.
While
they
effective
in
relieving
chronic
and
acute
pain,
their
narrow
window
of
therapeutic
use
contributes
to
the
high
occurrence
abuse.
The
associated
abuse
this
family
drugs
can
be
correlated
increase
dependency,
overdose,
death
users.
negative
effects
extend
beyond
physical
psychological
experienced
by
user
unregulated
synthesis
sale,
which
contribute
socioeconomic
challenges
a
biproduct
global
public
health
epidemic.
From
clinical
point-of-care
applications,
detection
real-time
monitoring
drug
is
critical
fight
decrease
improve
settings.
Chromatographic
separations
chromatography–mass
spectrometry
traditional
methods
opioid
analyses,
but
cost,
long
analysis
time,
absence
portability
highlight
need
for
development
fast,
situ,
analysis,
community
services.
This
review
highlights
recent
electrochemical
optical
(FTIR,
Raman,
colorimetric,
fluorescent)
advances
biosensors
pharmaceutical
illicit
analysis.
Specifically,
an
emphasis
placed
on
metabolites
biological
samples
vitro
cellular
assays
diagnosis
forensic
applications.
prospects
role
sensors,
biosensors,
sensors
promoting
diagnosis,
study,
point-of-care,
services
reduce
harm
also
provided.
Journal of the American Chemical Society,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 10, 2025
Monoacylglycerol
lipase
(MAGL)
is
the
pivotal
catabolic
enzyme
responsible
for
signal
termination
in
endocannabinoid
system.
Inhibition
of
MAGL
offers
unique
advantages
over
direct
activation
cannabinoid
receptors
treating
cancer,
metabolic
disorders,
and
inflammatory
diseases.
Although
specific
fluorescent
molecular
imaging
probes
are
commonly
used
real-time
analysis
localization
distribution
drug
targets
cells,
they
almost
invariably
composed
a
linker
connecting
pharmacophore
with
large
fluorophore.
In
this
study,
we
have
developed
miniaturized
targeting
by
incorporating
highly
boron-dipyrromethene
(BODIPY)
moiety
into
inhibitor
structure
that
interacts
active
site.
These
exhibit
favorable
drug-like
properties
such
as
high
solubility
permeability,
picomolar
potency
across
various
species,
cell
selectivity
specificity.
A
range
translational
investigations
were
conducted,
including
cell-free
fluorescence
polarization
assays,
fluorescence-activated
sorting
analysis,
confocal
microscopy
live
cancer
primary
neurons,
human-induced
pluripotent
stem
cell-derived
brain
organoids.
Furthermore,
application
red-shifted
analogs
or
18F
positron
emission
labeling
illustrated
significant
versatility
adaptability
ligands
experimental
contexts.
Analytical Methods,
Год журнала:
2024,
Номер
16(36), С. 6119 - 6133
Опубликована: Янв. 1, 2024
This
paper
provides
a
comprehensive
overview
of
fluorescent
probes
for
Ga
3+
,
addressing
challenges
and
proposing
corresponding
solutions,
while
also
outlining
future
directions
the
development
highly
efficient
probes.
MedComm – Biomaterials and Applications,
Год журнала:
2025,
Номер
4(1)
Опубликована: Янв. 19, 2025
Abstract
Escherichia
coli
(
E.
)
and
Salmonella
enteritidis
S.
are
common
food‐borne
pathogens,
which
pose
a
very
significant
threat
to
the
healthcare
environment.
The
rapid
detection
of
relevant
bacteria
can
help
control
their
spread,
while
traditional
bacterial
culture
method
is
time‐consuming
not
conducive
pathogens.
Recently,
new
methods
for
related
pathogenic
have
emerged,
but
these
relatively
complex,
few
detect
two
at
same
time.
Therefore,
there
an
urgent
need
develop
multi‐target,
convenient,
fast
pathogen
methods.
This
successfully
constructed
enzyme‐free
fluorescent
biosensor
based
on
adapter‐mediated
strand
displacement
reaction
ATCC25922
ATCC13076.
had
ultrasensitive
limit
0.7
CFU/mL
0.61
within
20
min,
with
broad
linear
range
34–10
5
17–10
6
,
respectively.
Importantly,
spiked
recovery
three
clinical
fluid
samples
performed
well,
proved
that
this
potential
in
samples.
sensor
by
dual
targets
time,
increasing
possibility
large‐scale
use.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 21, 2025
Ferroptosis
is
a
type
of
cell
death
triggered
by
the
iron-dependent
accumulation
lipid
peroxides
in
cells.
Diabetes,
chronic
metabolic
disorder
characterized
hyperglycemia,
can
lead
to
various
health
complications.
The
process
ferroptosis
and
progression
diabetes
are
closely
linked
redox
homeostasis,
which
regulated
levels
reactive
oxygen
sulfur
species.
Currently,
there
no
fluorescent
probes
available
monitor
changes
homeostasis
during
diabetes.
Here,
we
report
first
endeavor
create
reversible
near-infrared
fluorogenic
(NIRF)
probe
for
monitoring
reversal
precise
diagnosis.
In
vitro
data
demonstrated
that
NIR-CSTe
could
cyclically
reversibly
detect
ONOO-
GSH
up
four
times
with
minimal
loss
fluorescence
intensity.
With
help
NIR-CSTe,
observed
HT-1080
cells,
induced
undergo
erastin
after
being
washed
PBS
24
h
then
treated
ferrostatin-1,
showed
recovery
intracellular
levels.
contrast,
treatment
deferoxamine
did
not
yield
similar
results.
Lastly,
was
also
utilized
early
diagnosis
efficacy
assessment
relation
ONOO-/GSH
balance,
results
illustrating
combined
administration
metformin
empagliflozin
more
effective
than
using
either
drug
alone.
Thus,
this
smart
holds
significant
potential
as
an
essential
tool
clinical
diseases
associated
homeostasis.
