A rare PRIMER cell state in plant immunity

Nature, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Abstract Plants lack specialized and mobile immune cells. Consequently, any cell type that encounters pathogens must mount responses communicate with surrounding cells for successful defence. However, the diversity, spatial organization function of cellular states in pathogen-infected plants are poorly understood 1 . Here we infect Arabidopsis thaliana leaves bacterial trigger or supress integrate time-resolved single-cell transcriptomic, epigenomic transcriptomic data to identify states. We describe cell-state-specific gene-regulatory logic involves transcription factors, putative cis -regulatory elements target genes associated disease immunity. show a rare population emerges at nexus immune-active hotspots, which designate as primary responder (PRIMER) PRIMER have non-canonical signatures, exemplified by expression genome accessibility previously uncharacterized factor, GT-3A, contributes plant immunity against pathogens. surrounded another state (bystander) activates long-distance cell-to-cell signalling. Together, our findings suggest interactions between these propagate across leaf. Our molecularly defined spatiotemporal atlas provides functional regulatory insights into plants.

Язык: Английский

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Decoding Plant–Pathogen Interactions: A Comprehensive Exploration of Effector–Plant Transcription Factor Dynamics

Molecular Plant Pathology, Год журнала: 2025, Номер 26(1)

Опубликована: Янв. 1, 2025

ABSTRACT In the coevolutionary process between plant pathogens and hosts, pathogen effectors, primarily proteinaceous, engage in interactions with host proteins, such as transcription factors (TFs), during infection process. This review delves into intricate interplay TFs a key aspect prolonged complex battle plants pathogens. Effectors strategically manipulate using diverse tactics. These include modulating activity of TFs, influencing their incorporation multimeric complexes, directly changing TF expression levels, promoting degradation via ubiquitin‐proteasome system, inducing subcellular relocalization. The systematically presents documented interactions, elucidating mechanisms profound impact on host–pathogen dynamics. It emphasises central role defence investigates convergent evolution effectors targeting TFs. By providing this overview, we offer valuable insights dynamic interaction landscape suggest potential directions for future research.

Язык: Английский

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Function analysis of the cytidine deaminases in root-knot nematode, Meloidogyne incognita

European Journal of Plant Pathology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Язык: Английский

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The Candida quercitrusa strain Cq-C08 induces plant resistance to root-knot nematodes

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Апрель 17, 2025

Root-knot nematodes (RKNs), belonging to the genus Meloidogyne, are plant parasitic with a broad host range, causing substantial economic losses annually. The selection and utilization of novel biological control resources crucial for managing RKNs diseases. This study isolated Candida quercitrusa Cq-C08 from rhizosphere soil, which efficacy against Meloidogyne incognita was investigated through laboratory experiments, pot plot trials, analysis transcriptomic data cucumber roots treated Cq-C08. series experiments confirmed that fermentation broth had lethal rate 100% M. J2s within 12 h exhibited significant repellent effect on nematodes. In tests, strain achieved over 50% significantly promoted (Cucumis sativus, Zhongnong No. 6, China) growth. Inoculation could activate key immune signaling pathways salicylic acid (SA) jasmonic (JA). Split-root tests showed induced resistance by 32.3%. Comparative transcriptome regulate host's basal response oxidative burst SA, JA, ethylene (ET) pathways, alter secondary metabolism, activating synthesis cucurbitacin auxins, promotes regulation These results prove C. has high effects potential be developed into product root-knot

Язык: Английский

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NRPS-like ATRR in Plant-Parasitic Nematodes Involved in Glycine Betaine Metabolism to Promote Parasitism

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(8), С. 4275 - 4275

Опубликована: Апрель 12, 2024

Plant-parasitic nematodes (PPNs) are among the most serious phytopathogens and cause widespread damage in major crops. In this study, using a genome mining method, we identified nonribosomal peptide synthetase (NRPS)-like enzymes genomes of plant-parasitic nematodes, which conserved with two consecutive reducing domains at N-terminus (A-T-R1-R2) homologous to fungal NRPS-like ATRR. We experimentally investigated roles enzyme (MiATRR) nematode (Meloidogyne incognita) parasitism. Heterologous expression Miatrr Saccharomyces cerevisiae can overcome growth inhibition caused by high concentrations glycine betaine. RT-qPCR detection shows that is significantly upregulated early parasitic life stage (J2s plants) M. incognita. Host-derived RNA interference (RNAi) Arabidopsis thaliana decrease number galls egg masses incognita, as well retard development reduce body size nematode. Although exogenous betaine choline have no obvious impact on survival free-living incognita J2s (pre-parasitic J2s), they performance planta, especially Miatrr-RNAi plants. Following application rhizosphere soil A. thaliana, numbers were obviously reduced but increased choline. Based knowledge about function ATRR host plants suggest MiATRR involved nematode–plant interaction acting reductase, converting This may be universal strategy utilizing promote their parasitism

Язык: Английский

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Harnessing nematode-responsive promoters: A promising solution for plant parasitic nematodes management

Plant Stress, Год журнала: 2025, Номер unknown, С. 100835 - 100835

Опубликована: Март 1, 2025

Язык: Английский

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The nematicidal activity of Bacillus thuringiensis Cry1Ia36 expressing in Escherichia coli

Pesticide Biochemistry and Physiology, Год журнала: 2025, Номер unknown, С. 106419 - 106419

Опубликована: Апрель 1, 2025

Язык: Английский

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Combating Root-Knot Nematodes (Meloidogyne spp.): From Molecular Mechanisms to Resistant Crops

Plants, Год журнала: 2025, Номер 14(9), С. 1321 - 1321

Опубликована: Апрель 27, 2025

Root-knot nematodes (RKNs; Meloidogyne spp.) are significant plant–parasitic that cause major yield losses worldwide. With growing awareness of the harmful effects chemical pesticides on human health and environment, there is an urgent need to develop alternative strategies for controlling RKN in agricultural fields. In recent years, implementing multiple approaches based transcriptomics, genomics, genome engineering, including modern platforms like CRISPR/Cas9, along with traditional genetic mapping, has led great advances understanding plant–RKN interactions underlying molecular mechanisms plant resistance. this literature review, we synthesize contributions relevant studies field discuss key findings. This includes, instance, transcriptomics helped expand our RKN-resistance mechanisms, overexpression hormone-related genes, silencing susceptibility genes lead review was conducted by searching scientific sources, PubMed Google Scholar, publications filtering them using keywords such as RKN–plant defense host–plant resistance against RKN, mapping RKN. knowledge can be leveraged accelerate development RKN-resistant plants substantially improve management economically important crops.

Язык: Английский

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The root‐knot nematode effector MiEFF12 targets the host ER quality control system to suppress immune responses and allow parasitism

Molecular Plant Pathology, Год журнала: 2024, Номер 25(7)

Опубликована: Июль 1, 2024

Abstract Root‐knot nematodes (RKNs) are microscopic parasitic worms able to infest the roots of thousands plant species, causing massive crop yield losses worldwide. They evade plant's immune system and manipulate cell physiology metabolism transform a few root cells into giant cells, which serve as feeding sites for nematode. RKN parasitism is facilitated by secretion in planta effector molecules, mostly proteins that hijack host cellular processes. We describe here conserved RKN‐specific effector, 12 (EFF12), synthesized exclusively oesophageal glands nematode, we demonstrate its function parasitism. In plant, MiEFF12 localizes endoplasmic reticulum (ER). A combination RNA‐sequencing analysis immunity‐suppression bioassays revealed contribution modulation immunity. Yeast two‐hybrid, split luciferase co‐immunoprecipitation approaches identified an essential component ER quality control system, Solanum lycopersicum bap‐like (PBL), basic leucine zipper 60 (BZIP60) targets MiEFF12. Finally, silencing PBL genes Nicotiana benthamiana decreased susceptibility Meloidogyne incognita infection. Our results suggest EFF12 manipulates modify responses allow

Язык: Английский

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Silencing CsMAP65-2 and CsMAP65-3 in cucumber reduces susceptibility to Meloidogyne incognita

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 219, С. 109356 - 109356

Опубликована: Ноя. 29, 2024

Язык: Английский

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