A Systematic Method for the Identification of Oligosaccharide Constituents in Polygonatum cyrtonema Hua Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry

Molecules, Год журнала: 2025, Номер 30(7), С. 1433 - 1433

Опубликована: Март 24, 2025

A Polygonatum cyrtonema Hua (PCH) is a common medicinal and edible plant whose rhizomes are widely used for the treatment prevention of various diseases. Previous studies have revealed variety chemical components such as polysaccharides, saponins, flavonoids, which possess biological activities antimicrobial, lipid-regulating, anti-aging, hypoglycemic, anti-inflammatory. However, to date, structure activity its oligosaccharide still unclear. In this study, we developed method combining ultra-high-performance liquid chromatography with Q-Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive MS) monosaccharide analysis identification oligosaccharides in PCH. Finally, total 44 oligosaccharides, including 27 fructo-oligosaccharides (FOS), 10 arabino-oligosaccharides (AOS), 7 others, were identified based on precise relative molecular fragment ion information provided by high-resolution spectrometry, combination standard comparison, composition analysis, relevant literature reports. All those reported first time. These findings laid foundation subsequent study substances theoretical basis more comprehensive development utilization PCH product.

Язык: Английский

Gut Microbiota Modulation in IBD: From the Old Paradigm to Revolutionary Tools

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3059 - 3059

Опубликована: Март 27, 2025

Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders primarily comprising two main conditions: ulcerative colitis and Crohn’s disease. The gut microbiota’s role in driving inflammation IBD has garnered significant attention, yet the precise mechanisms through which microbiota influences pathogenesis remain largely unclear. Given limited therapeutic options for IBD, alternative microbiota-targeted therapies—including prebiotics, probiotics, postbiotics, symbiotics—have been proposed. While these approaches have shown promising results, modulation is still mainly considered an adjunct therapy to conventional treatments, with a demonstrated impact on patients’ quality of life. Fecal transplantation (FMT), already approved treating Clostridioides difficile infection, represents first series innovative microbiota-based therapies under investigation. Microbial biotherapeutics emerging as personalized cutting-edge tools management, encompassing next-generation bacterial consortia, bacteriophages, engineered direct metabolic pathway modulation, nanotherapeutics. This review explores microbial strategy IBDs, highlighting current examining development better understand their potential clinical applications managing intestinal disorders.

Язык: Английский

3-Fucosyllactose alleviates DSS-induced mouse colitis through modulation of the PI3K-Akt signaling pathway: An integrated multi-omics analysis

Journal of Functional Foods, Год журнала: 2025, Номер 127, С. 106789 - 106789

Опубликована: Апрель 1, 2025

Язык: Английский