Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Июль 22, 2022
Biocatalytic
cascades
play
a
fundamental
role
in
sustainable
chemical
synthesis.
Fusion
enzymes
are
one
of
the
powerful
toolboxes
to
enable
tailored
combination
multiple
for
efficient
cooperative
cascades.
Especially,
this
approach
offers
substantial
potential
practical
application
cofactor-dependent
oxidoreductases
by
forming
cofactor
self-sufficient
Adequate
recycling
while
keeping
oxidized/reduced
confined
microenvironment
benefits
from
fusion
fashion
and
makes
use
harsh
non-aqueous
media
practical.
In
mini-review,
we
have
summarized
various
aqueous
with
focus
on
discussion
linker
design
within
oxidoreductases.
The
properties
reported
linkers
been
reviewed
detail.
Besides,
substrate
loadings
these
studies
listed
showcase
key
limitations
(low
solubility
hydrophobic
substrates)
biocatalysis
when
it
comes
efficiency
economic
feasibility.
Therefore,
straightforward
strategy
applying
has
briefly
discussed
using
oxidoreductase
interest
organic
was
highlighted.
Green Chemistry,
Год журнала:
2022,
Номер
24(8), С. 3225 - 3234
Опубликована: Янв. 1, 2022
Biocatalytic
cascades
were
developed
to
synthesize
15
benzylisoquinoline
alkaloids
in
excellent
ee
values
from
dopamine
and
p
-coumaric
acid
derivatives.
Microbial Biotechnology,
Год журнала:
2022,
Номер
15(5), С. 1622 - 1632
Опубликована: Янв. 27, 2022
Summary
The
fluorinase
enzyme
represents
the
only
biological
mechanism
capable
of
forming
stable
C–F
bonds
characterized
in
nature
thus
far,
offering
a
biotechnological
route
to
biosynthesis
value‐added
organofluorines.
is
known
operate
hexameric
form,
but
consequence(s)
oligomerization
status
on
activity
and
its
catalytic
properties
remain
largely
unknown.
In
this
work,
aspect
was
explored
by
rationally
engineering
trimeric
variants
that
retained
same
rate
as
wild‐type
enzyme.
These
results
ruled
out
hexamerization
requisite
for
fluorination
activity.
Michaelis
constant
(
K
M
)
S
‐adenosyl‐
l
‐methionine,
one
substrates
fluorinase,
increased
two
orders
magnitude
upon
hexamer
disruption.
Such
shift
‐methionine
affinity
points
long‐range
effect
substrate
binding
–
likely
decreasing
dissociation
release
from
active
site.
A
practical
application
illustrated
establishing
vitro
fluorometabolite
synthesis
bacterial
cell‐free
system.
Abstract
5‐(Hydroxymethyl)furfural
(HMF)
is
a
key
platform
chemical
derived
from
renewable
biomass
sources,
holding
great
potential
as
starting
material
for
the
synthesis
of
valuable
compounds,
thereby
replacing
petrochemical‐derived
counterparts.
Among
these
valorised
2,5‐furandicarboxylic
acid
(FDCA)
has
emerged
versatile
building
block.
Here
we
demonstrate
biocatalytic
FDCA
HMF
via
one‐pot
three‐step
oxidative
cascade
performed
two
operative
steps
under
mild
reaction
conditions
employing
unspecific
peroxygenases
(UPOs)
using
hydrogen
peroxide
only
oxidant.
The
challenge
oxidation
by
UPOs
chemoselectivity
first
step,
one
possible
products
poor
substrate
further
oxidation.
peroxygenase
Marasmius
oreades
(
Mor
UPO)
was
found
to
oxidize
100
mM
5‐formyl‐2‐furoic
(FFCA)
with
95
%
chemoselectivity.
In
sequential
UPO
(TON
up
13535)
and
Agrocybe
aegerita
Aae
UPO,
TON
7079),
were
oxidized
reaching
99
conversion
yielding
861
mg
isolated
pure
crystalline
FDCA,
presenting
example
gram
scale
involving
UPOs.
Synthetic biology and engineering,
Год журнала:
2024,
Номер
2(1), С. 10004 - 10004
Опубликована: Янв. 1, 2024
Green
and
efficient
biocatalytic
technology
has
become
a
complementary
or
alternative
means
of
organic
synthesis.
Chemicals
with
two
functional
groups,
such
as
α,ω-dicarboxylic
acids,
ω-amino
fatty
acids
ω-hydroxy
are
widely
used
in
the
synthesis
polymers
polyesters
polyamides.
In
recent
years,
production
biodegradable
materials
using
renewable
abundant
vegetable
oils
green
raw
attracted
increasing
attention,
receiving
an
additional
impetus
from
synthetic
biology.
This
paper
presents
research
progress
bifunctional
chemicals
medium
chain
lengths
C8–C12
multi-enzyme
cascades.
Recent
studies
have
developed
multilevel
optimization
strategies
to
improve
efficiency,
economics,
sustainability
Cofactor
regeneration
were
avoid
large
additions
expensive
coenzymes.
Protein
engineering
applied
enzyme
stability
catalytic
performance.
addition,
blocking
β-oxidation
pathway,
improving
efficiency
substrate
transport
across
membranes
cellular
robustness
effective
for
whole-cell
systems.
we
discuss
development
prospects
producing
high
value-added
fine
one-pot
reaction
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Июль 22, 2022
Biocatalytic
cascades
play
a
fundamental
role
in
sustainable
chemical
synthesis.
Fusion
enzymes
are
one
of
the
powerful
toolboxes
to
enable
tailored
combination
multiple
for
efficient
cooperative
cascades.
Especially,
this
approach
offers
substantial
potential
practical
application
cofactor-dependent
oxidoreductases
by
forming
cofactor
self-sufficient
Adequate
recycling
while
keeping
oxidized/reduced
confined
microenvironment
benefits
from
fusion
fashion
and
makes
use
harsh
non-aqueous
media
practical.
In
mini-review,
we
have
summarized
various
aqueous
with
focus
on
discussion
linker
design
within
oxidoreductases.
The
properties
reported
linkers
been
reviewed
detail.
Besides,
substrate
loadings
these
studies
listed
showcase
key
limitations
(low
solubility
hydrophobic
substrates)
biocatalysis
when
it
comes
efficiency
economic
feasibility.
Therefore,
straightforward
strategy
applying
has
briefly
discussed
using
oxidoreductase
interest
organic
was
highlighted.
