Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 23, 2024
Thermal-responsive
hydrogels
are
developed
as
ion-conductive
switchs
for
energy
storage
devices,
however,
the
molecule
mechanism
of
switch
on/off
remains
unclear.
Here,
poly(N-isopropylacrylamide-co-acrylamide)
hydrogel
is
synthesized
a
model
material
and
nanodiamond
(ND)
based
quantum
sensing
phase
change
study
developed.
First,
micro-scale
separation
with
cross-linked
mesh
structure
after
sol-gel
transition
visualized
in
situ
water
molecules
trapped
by
polymer
chains
on
chemically
"frozen"
state.
Then,
nano-scale
inhomogeneous
distributions
viscosity,
thermal
conductivity
ionic
mobility
at
high
temperature
observed
measuring
rotation,
translation
zero-field
splitting
NDs.
Besides,
found
to
be
dependent
not
only
but
also
concentration.
These
observations
suggested
that
physical
"wall"
induced
microscopic
scale
blocked
ion
conduction
pathways,
providing
potential
intrinsic
explanation
migration
shut-down
temperature.
ACS Sensors,
Год журнала:
2024,
Номер
9(7), С. 3511 - 3519
Опубликована: Апрель 23, 2024
Excavating
nucleic
acid
quantitative
capabilities
by
combining
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
and
isothermal
amplification
in
one
pot
is
of
common
interest.
However,
the
mutual
interference
between
CRISPR
cleavage
primary
obstacle
to
detection.
Though
several
works
have
demonstrated
enhanced
detection
sensitivity
reducing
inhibition
on
pot,
few
paid
attention
process
even
dynamic
reaction
processes
two.
Herein,
we
find
that
DNA
quantification
can
be
realized
regulating
either
recombinase
polymerase
(RPA)
efficiency
or
CRISPR/Cas12a
cleaving
(namely,
tuning
balance)
pot.
The
sensitive
utilizing
dual
PAM-free
crRNAs
for
recognition.
varied
RPA
primer
concentration
with
stabilized
systems
significantly
affects
performances.
Alternatively,
also
achieved
stabilizing
while
concentration.
capability
proved
detecting
targets
from
Lactobacillus
acetotolerans
SARS-CoV-2.
performance
toward
real
samples
comparable
real-time
PCR
L.
spiked
fermented
food
SARS-CoV-2
clinical
samples.
We
expect
presented
method
will
a
powerful
tool
quantifying
other
targets.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Abstract
Microfluidic
droplets,
with
their
unique
properties
and
broad
applications,
are
essential
in
chemical,
biological,
materials
synthesis
research.
Despite
the
flourishing
studies
on
artificial
intelligence‐accelerated
microfluidics,
most
research
efforts
have
focused
upstream
design
phase
of
microfluidic
systems.
Generating
user‐desired
droplets
still
remains
laborious,
inefficient,
time‐consuming.
To
address
long‐standing
challenges
associated
accurate
efficient
identification,
sorting,
analysis
morphology
generation
rate
single
double
emulsion
a
novel
machine
vision
approach
utilizing
deformable
detection
transformer
(DETR)
algorithm
is
proposed.
This
method
enables
rapid
precise
(detection
relative
error
<
4%
precision
>
94%)
across
various
scales
scenarios,
including
real‐world
simulated
environments.
identification
(MDIA),
web‐based
tool
powered
by
Deformable
DETR,
which
supports
transfer
learning
to
enhance
accuracy
specific
user
scenarios
developed.
MDIA
characterizes
diameter,
number,
frequency,
other
parameters.
As
more
training
data
added
users,
MDIA's
capability
universality
expand,
contributing
comprehensive
database
for
droplet
microfluidics.
The
work
highlights
potential
intelligence
advancing
regulation,
fabrication,
label‐free
analysis,
accelerating
biochemical
sciences
engineering.
ACS Synthetic Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 2, 2025
We
report
here
the
use
of
antibody-DNA
conjugates
(Ab-DNA)
to
activate
collateral
cleavage
activity
CRISPR-Cas12a
enzyme.
Our
findings
demonstrate
that
Ab-DNA
effectively
trigger
CRISPR-Cas12a,
enabling
transduction
antibody-mediated
recognition
events
into
fluorescence
outputs.
developed
two
different
immunoassays
using
an
as
activator
Cas12a:
CRISPR-based
immunosensing
assay
(CIA)
for
detecting
SARS-CoV-2
spike
S
protein,
which
shows
superior
sensitivity
compared
with
traditional
enzyme-linked
immunosorbent
(ELISA),
and
immunomagnetic
(CIMA).
Notably,
CIMA
successfully
detected
protein
in
undiluted
saliva
a
limit
detection
(LOD)
890
pM
2
h
assay.
results
underscore
benefits
integrating
Cas12a-based
signal
amplification
antibody
methods.
The
potential
conjugates,
combined
CRISPR
technology,
offers
promising
alternative
conventional
enzymes
used
could
facilitate
development
versatile
analytical
platforms
non-nucleic
acid
targets.
ACS Nano,
Год журнала:
2024,
Номер
18(35), С. 24236 - 24251
Опубликована: Авг. 22, 2024
CRISPR/Cas-based
molecular
diagnosis
demonstrates
potent
potential
for
sensitive
and
rapid
pathogen
detection,
notably
in
SARS-CoV-2
mutation
tracking.
Yet,
a
major
hurdle
hindering
widespread
practical
use
is
its
restricted
throughput,
limited
integration,
complex
reagent
preparation.
Here,
system,
microfluidic
multiplate-based
ultrahigh
throughput
analysis
of
variants
concern
using
CRISPR/Cas12a
nonextraction
RT-LAMP
(mutaSCAN),
proposed
detection
with
resource
requirements.
With
the
aid
self-developed
reagents
deep-learning
enabled
prototype
device,
our
mutaSCAN
system
can
detect
mock
swab
samples
below
30
min
as
low
250
copies/mL
up
to
96
per
round.
Clinical
specimens
were
tested
this
accuracy
routine
testing
(22
wildtype
samples,
26
mutational
samples)
was
98%
100%,
respectively.
No
false-positive
results
found
negative
(n
=
24)
samples.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(8), С. 6894 - 6894
Опубликована: Апрель 7, 2023
Genome
editing
technologies
that
are
currently
available
and
described
have
a
fundamental
impact
on
the
development
of
molecular
biology
medicine,
industrial
agricultural
biotechnology
other
fields.
However,
genome
based
detection
manipulation
targeted
RNA
is
promising
alternative
to
control
gene
expression
at
spatiotemporal
transcriptomic
level
without
complete
elimination.
The
innovative
CRISPR-Cas
RNA-targeting
systems
changed
conception
biosensing
also
allowed
effectors
be
used
in
various
applications;
for
example,
genomic
editing,
effective
virus
diagnostic
tools,
biomarkers,
transcription
regulations.
In
this
review,
we
discussed
current
state-of-the-art
specific
known
bind
cleave
substrates
summarized
potential
applications
versatile
systems.
Analytical Methods,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Reviews
the
development
of
visual
detection
methods
in
recent
years
based
on
different
Cas
and
analyzes
their
advantages
disadvantages
as
well
challenges
future
research.
