Identification and mechanistic exploration of key anti‐inflammatory molecules in American ginseng: Impacts on signal transducer and activator of transcription 3 STAT3 phosphorylation and macrophage polarization

Phytotherapy Research, Год журнала: 2024, Номер 38(8), С. 4307 - 4320

Опубликована: Июль 8, 2024

Abstract American ginseng (AG) has been reported to have anti‐inflammatory effects in many diseases, but the key molecules and mechanisms are unclear. This study aims evaluate mechanism of AG identify by vivo vitro models. Zebrafish was employed assess properties compounds. Metabolomics utilized potential AG, while molecular dynamics simulations were conducted forecast interaction capabilities these compounds with inflammatory targets. Additionally, macrophage cell investigate enzyme‐linked immunosorbent assay western blotting. Seven discovered ginsenoside Rg1, Rs3 (G‐Rs3), oleanolic acid exhibiting strongest affinity for signal transducer activator transcription 3. These demonstrated inhibitory on migration zebrafish models ability regulate ROS levels both macrophages. The experiments found that Rs3, could promote M2/M1 polarization ratio inhibit phosphorylation overexpression revealed provided new evidence scientific use AG.

Язык: Английский

---

Pyraclostrobin induces developmental toxicity and cardiotoxicity through oxidative stress and inflammation in zebrafish embryos

Environmental Pollution, Год журнала: 2024, Номер 358, С. 124490 - 124490

Опубликована: Июль 1, 2024

Язык: Английский

---

Exposure of male adult zebrafish (Danio rerio) to triphenyl phosphate (TPhP) induces eye development disorders and disrupts neurotransmitter system-mediated abnormal locomotor behavior in larval offspring

Journal of Hazardous Materials, Год журнала: 2023, Номер 465, С. 133332 - 133332

Опубликована: Дек. 22, 2023

Язык: Английский

---

Shortening the early diagnostic window of Hg2+-induced liver injury with a H2O2-activated fluorescence/afterglow imaging assay

Journal of Hazardous Materials, Год журнала: 2024, Номер 480, С. 136059 - 136059

Опубликована: Окт. 4, 2024

Язык: Английский

---

Soybean hull polysaccharides ameliorate lipid metabolic disorders and gut microbiota dysbiosis in high-fat diet-fed zebrafish

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 141123 - 141123

Опубликована: Фев. 1, 2025

Язык: Английский

---

Methodological and Ethical Considerations in the Use of Chordate Embryos in Biomedical Research

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2624 - 2624

Опубликована: Март 14, 2025

Animal embryos are vital tools in scientific research, providing insights into biological processes and disease mechanisms. This paper explores their historical contemporary significance, highlighting the shift towards refinement of vitro systems as alternatives to animal experimentation. We have conducted a data review relevant literature on use research synthesized highlight importance this model for progress ethical considerations regulations surrounding embryo emphasizing minimizing suffering while promoting through principles replacement, reduction, refinement. Embryos from wide range species, including mammals, fish, birds, amphibians, reptiles, play crucial experimental role enabling us understand factors such substance toxicity, embryonic development, metabolic pathways, physiological processes, etc., that contribute advancement sciences. To apply effectively, it is essential match objectives with most appropriate methodology, ensuring chosen approach scope study.

Язык: Английский

---

Carrier‐free co‐delivery nanosystem based on prothioconazole and pyrimethanil to enhance fungicidal efficacy and reduce environmental risks

Pest Management Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

The conventional application of fungicides suffers from substantial off-target loss due to photolysis, evaporation, and rain washing, resulting in risks the environment human health. To address these limitations, self-assembled nanopesticides based on two kinds have emerged as precision agrochemical delivery platforms that synchronize enhanced bioactivity with environmental risk mitigation. Herein, core-shell structured nanoparticles (PRO-PYR NPs) an average particle size 263 nm were fabricated prothioconazole (PRO) pyrimethanil (PYR) via non-covalent interactions using co-assembly technology. co-delivery system acid-responsive release property both antifungal activities against Botrytis cinerea Fusarium graminearum duration cinerea. Importantly, obtained PRO-PYR NPs could significantly improve photostability foliage adhesion reduce their volatility leaching potential. Additionally, reduced genotoxicity PRO PYR root tip cells Vicia faba acute toxicity zebrafish. commonly used successfully prepared by technology effectively physicochemical properties. Carrier-free strategy multiple components enhance control effect lower negative impacts associated non-target organisms for integrated pest management. © 2025 Society Chemical Industry.

Язык: Английский

---

Zebrafish as a model for olfactory research: A systematic review from molecular mechanism to technology application

Food Chemistry, Год журнала: 2025, Номер unknown, С. 144698 - 144698

Опубликована: Май 1, 2025

Язык: Английский

---

A Multi-Endpoint Approach to Ecotoxicological Assessment of Wastewater Polluted Rivers Using Zebrafish

Environmental Research, Год журнала: 2025, Номер unknown, С. 121996 - 121996

Опубликована: Июнь 1, 2025

Язык: Английский

---

Human‐based new approach methodologies to accelerate advances in nutrition research

Food Frontiers, Год журнала: 2024, Номер 5(3), С. 1031 - 1062

Опубликована: Март 5, 2024

Abstract Much of nutrition research has been conventionally based on the use simplistic in vitro systems or animal models, which have extensively employed an effort to better understand relationships between diet and complex diseases as well evaluate food safety. Although these models undeniably contributed increase our mechanistic understanding basic biological processes, they do not adequately model human physiopathological phenomena, creating concerns about translatability humans. During last decade, extraordinary advancement stem cell culturing, three‐dimensional cultures, sequencing technologies, computer science occurred, originated a wealth novel human‐based more physiologically relevant tools. These tools, also known “new approach methodologies,” comprise patient‐derived organoids, organs‐on‐chip, multi‐omics approach, along with computational analysis, represent innovative exciting tools forward from human‐biology‐oriented perspective. After considering some shortcomings conventional vivo approaches, here we describe main available emerging that are appropriate for designing human‐relevant research. Our aim is encourage discussion opportunity explore paths promote paradigm‐change toward biology‐focused nutritional pathophysiology, products, develop effective targeted preventive therapeutic strategies while helping reducing number replacing animals

Язык: Английский

---