Photosynthetic performance, pigments and biochemicals influence seed yield in rapeseed under water deficit conditions: MGIDI index helps screening drought-tolerant genotypes

Field Crops Research, Год журнала: 2025, Номер 322, С. 109733 - 109733

Опубликована: Янв. 9, 2025

Язык: Английский

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The MYB transcription factor RtAN2 promotes anthocyanin accumulation over proanthocyanidin during the fruit ripening of rose myrtle berries

Industrial Crops and Products, Год журнала: 2025, Номер 224, С. 120433 - 120433

Опубликована: Янв. 5, 2025

Язык: Английский

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Spatial and Temporal Regulation of Flower Coloration in Cymbidium lowianum

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Янв. 20, 2025

ABSTRACT Flower color is a crucial trait that attracts pollinators and determines the ornamental value of floral crops. Cymbidium lowianum , one most important breeding parent hybrids, has two flower morphs (normal albino) differ in lip color. However, molecular mechanisms underlying formation C. are not well understood. In this study, comparative metabolomic analysis between normal albino tissues indicated cyanidin‐3‐ O ‐glucoside content was significantly higher red epichiles than other tissues. This finding suggests responsible for variation differentiation . We also found coloration correlated with high levels F3'H expression; further, anthocyanins, carotenoids chlorophyll coordinate to influence sepal petal during development. transgenic Arabidopsis lines, overexpression increased anthocyanin concentration, BCH carotenoid whereas HEMG CHLI both concentration. Identification assessment several transcription factors revealed MYB308‐1 activates MYB111 PIF4‐2 activate expression Importantly, MYB14‐1 shows interaction PIF4‐2, appears act as connector biosynthesis by either activating or inhibiting expression. These results indicate that, patterns primarily regulated types concentrations flavonoids;

Язык: Английский

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Developmental Mechanisms of Fruit Diversification in Angiosperms and the Evolutionary Implications

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

The evolutionary origin of fruits played a pivotal role in promoting the dominance angiosperms on Earth as protect and nourish seeds facilitate their dispersal through diverse mechanisms. Understanding molecular networks underlying fruit development is prerequisite for elucidating mechanisms shaping diversification, particularly improving crop yield quality response to rapid climate change modern agricultural systems. In this article, we offer comprehensive analysis classification, emphasising intrinsic characteristics adaptive strategies specific environments. Based studies model systems such Arabidopsis thaliana Solanum lycopersicum, highlight recent advances identifying novel components involved development. We further discuss that contribute diversification context well-established phylogenetic frameworks, with examples from Brassicaceae Solanaceae families. A comparison between indicates key module largely conserved evolution. propose future research integrates multidisciplinary evidence could help better understand which ultimately practice.

Язык: Английский

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Protein kinase SnRK2.6 phosphorylates transcription factor bHLH3 to regulate anthocyanin homeostasis during strawberry fruit ripening

Journal of Experimental Botany, Год журнала: 2024, Номер 75(18), С. 5627 - 5640

Опубликована: Май 29, 2024

Strawberry (Fragaria × ananassa) is a model plant for studying non-climacteric fruit ripening regulated by abscisic acid (ABA); however, the signaling of ABA in regulation coloration not fully understood. In this study, we identified transcription factor BASIC HELIX-LOOP-HELIX 3 (bHLH3) as being key to via yeast two-hybrid library screening using bait SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASE 2 (SnRK2.6), which core component that negatively regulates ripening. The interaction was also confirmed firefly luciferase complementation assays and pull-down assays. RT-qPCR western blot analysis bHLH3 expressed ubiquitously strawberry tissues, it stably during development. Overexpression RNAi both SnRK2.6 demonstrated promote inhibit coloration, respectively. Using EMSAs, showed promotes expression UDP-GLUCOSE: FLAVONOL-O-GLUCOSYLTRANSFERASE (UFGT), gene anthocyanin biosynthesis, directly binding its promoter. We determined can phosphorylate inhibits UFGT promoter, consequently suppressing expression. Altogether, propose increased content leads decreased SnRK2.6, turn releases phosphorylation thereby enhances expression, ultimately promoting fruit.

Язык: Английский

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Molecular mechanisms underlying natural deficient and ultraviolet‐induced accumulation of anthocyanin in the peel of ‘Jinxiu’ peach

Plant Cell & Environment, Год журнала: 2024, Номер 47(12), С. 4833 - 4848

Опубликована: Авг. 5, 2024

Abstract Peach varieties that differ in red coloration due to varied anthocyanin accumulation result from transcriptional regulation by PpMYB10s , a group of specific R2R3 MYBs. Here we investigated the mechanisms driving lack yellow‐skinned ‘Jinxiu’ peach peel, as well induced UV irradiance. It was found PpMYB10.1 PpMYB10.2 and PpMYB10.3 were positive regulators accumulation, but stimulation weak. Low expression causes natural deficiency peel. However, promoter sequences identical naturally red‐coloured ‘Hujingmilu’. Therefore, potential negative regulator(s) upstream explored. A novel R2R3‐MYB repressor termed PpMYB80 identified through comparative transcriptomic analysis then functionally confirmed via transiently overexpressing silencing fruit, transformation tobacco. directly binds inhibits its expression, does not affect . In UV‐exposed upregulated, while remained low enhanced, which results This study revealed cascade involving PpMYB activators repressors regulating basal UV‐induced

Язык: Английский

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Integrated transcriptomic and physio-molecular studies unveil the melatonin and PGPR induced protection to photosynthetic attributes in Brassica juncea L. under cadmium toxicity

Journal of Hazardous Materials, Год журнала: 2024, Номер 476, С. 134875 - 134875

Опубликована: Июнь 19, 2024

Язык: Английский

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Tracing the color: quantitative trait loci analysis reveals new insights into red-flesh pigmentation in apple (Malus domestica)

Horticulture Research, Год журнала: 2024, Номер 11(8)

Опубликована: Июнь 27, 2024

Abstract Red-flesh color development in apple fruit is known to depend upon a particular allele of the MdMYB10 gene. While anthocyanin metabolic pathway well characterized, current genetic models do not explain observed variations red-flesh pigmentation intensity. Previous studies focused on total content as phenotypic trait characterize overall flesh color. this approach led global understanding mechanisms involved expression, it essential adopt more quantitative approach, by analyzing other phenolic compound classes, order better understand molecular subtle variation and distribution. In study, we performed pedigree-based loci (QTL) mapping, using FlexQTL™ software, decipher determinism five F1 inter-connected families segregating for trait. A 452 genotypes were evaluated profiles during 3 years (2021–2023). We identified 24 QTLs intensity profiles. Six detected LG1, LG2, LG8, LG9, LG11, LG16. Several genes QTL confidence intervals related metabolism. Further analyses allowed us propose model which competition between anthocyanins flavan-3-ols (monomer oligomer) end-products decisive development. model, alleles favorable high can be inherited from both white-flesh parents.

Язык: Английский

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An ‘activator-repressor’ loop controls the anthocyanin biosynthesis in red-skinned pear

Molecular Horticulture, Год журнала: 2024, Номер 4(1)

Опубликована: Июль 1, 2024

Abstract The color of red-skinned pear ( Pyrus spp.) is primarily attributed to accumulation anthocyanins, which provide nutritional benefits for human health and are closely associated with the commercial value fruits. Here, we reported functional characterization a R2R3-MYB repressor PyMYB107, forms an ‘activator-repressor’ loop control anthocyanin in pear. PyMYB107 overexpression inhibited biosynthesis both calli fruits, while virus-induced gene silencing increased Furthermore, ectopic expression decreased tomato, strawberry tobacco. can competitively bind PybHLH3 PyMYB10/MYB114, thereby suppressing transcriptional activation key genes, PyANS PyUFGT . Site-directed mutagenesis showed that mutations within R3 domain EAR motif eliminated its repressive activity. Additionally, exhibited comparable pattern PyMYB10 / MYB114 was transcriptionally activated by them. Our finding advanced comprehension repression mechanism underlying accumulation, providing valuable molecular insights into improving quality

Язык: Английский

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Metabolomic and transcriptomic analyses provide insights into temperature and light regulated anthocyanin accumulation in flesh of ‘Furongli’ plum (Prunus salicina Lindl.)

Postharvest Biology and Technology, Год журнала: 2024, Номер 221, С. 113326 - 113326

Опубликована: Ноя. 27, 2024

Язык: Английский

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