Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

Nature Reviews Microbiology, Год журнала: 2019, Номер 18(2), С. 67 - 83

Опубликована: Дек. 19, 2019

Язык: Английский

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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors

Nature Biotechnology, Год журнала: 2020, Номер 38(7), С. 824 - 844

Опубликована: Июнь 22, 2020

Язык: Английский

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CRISPR-based diagnostics

Nature Biomedical Engineering, Год журнала: 2021, Номер 5(7), С. 643 - 656

Опубликована: Июль 16, 2021

Язык: Английский

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Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration

Nature, Год журнала: 2019, Номер 571(7764), С. 219 - 225

Опубликована: Июнь 12, 2019

Язык: Английский

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CRISPR-CasΦ from huge phages is a hypercompact genome editor

Science, Год журнала: 2020, Номер 369(6501), С. 333 - 337

Опубликована: Июль 17, 2020

Compact defense system in bacteriophages The CRISPR-Cas system, naturally found many prokaryotes, is widely used for genome editing. CRISPR arrays the bacterial genome, derived from of invading viruses, are to generate a RNA that guides Cas enzyme destroy repeat viral invaders. Recently, an unexpectedly compact was identified huge bacteriophages. Pausch et al . show even though this lacks commonly accessory proteins, it functional. In addition array, only component called CasF, which uses same active site process transcripts into and foreign nucleic acids. This human plant cells, provides hypercompact genome-editing toolbox. Science issue p. 333

Язык: Английский

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Clades of huge phages from across Earth’s ecosystems

Nature, Год журнала: 2020, Номер 578(7795), С. 425 - 431

Опубликована: Фев. 12, 2020

Bacteriophages typically have small genomes

Язык: Английский

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The emerging and uncultivated potential of CRISPR technology in plant science

Nature Plants, Год журнала: 2019, Номер 5(8), С. 778 - 794

Опубликована: Июль 15, 2019

Язык: Английский

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CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis

Nano Letters, Год журнала: 2020, Номер 21(1), С. 693 - 699

Опубликована: Дек. 21, 2020

Cell-free DNA (cfDNA) has attracted significant attention due to its high potential diagnose diseases, such as cancer. Still, detection by amplification method limitations because of false-positive signals and difficulty in designing target-specific primers. CRISPR-Cas-based fluorescent biosensors have been developed but also need the step for detection. In this study, first time CRISPR-Cas12a based nucleic acid amplification-free biosensor was detect cfDNA a metal-enhanced fluorescence (MEF) using DNA-functionalized Au nanoparticle (AuNP). Upon activating complex target subsequent single-strand (ssDNA) degradation between AuNP fluorophore, MEF occurred with color changes from purple red-purple. Using system, breast cancer gene-1 (BRCA-1) can be detected very sensitivity 30 min. This rapid highly selective sensor applied measure other biomarkers viral field-deployable point-of-care testing (POCT) platform.

Язык: Английский

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Increasing the specificity of CRISPR systems with engineered RNA secondary structures

Nature Biotechnology, Год журнала: 2019, Номер 37(6), С. 657 - 666

Опубликована: Апрель 15, 2019

Язык: Английский

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Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay

ACS Nano, Год журнала: 2020, Номер 14(2), С. 2497 - 2508

Опубликована: Фев. 11, 2020

The lateral flow assay is one of the most convenient analytical techniques for analyzing immune response, but its applicability to precise genetic analyses limited by false-positive signal and tedious inefficient hybridization steps. Here, we introduce CRISPR (clustered regularly interspaced short palindromic repeats) /Cas system into assay, termed CRISPR/Cas9-mediated nucleic acid (CASLFA), address such issues. In this study, CASLFA utilized identify Listeria monocytogenes, genetically modified organisms (GMOs), African swine fever virus (ASFV) at a detection limit hundreds copies genome samples with high specificity within 1 h. We further evaluated performance in nonlaboratory environment successfully confirmed 27 ASFV-infected from 110 suspected serum samples, an accuracy 100% when compared real-time PCR (RT-PCR) assay. satisfies some characteristics next-generation molecular diagnostics tool due rapidity accuracy, allowing point-of-care use without need technical expertise complex ancillary equipment. This method has great potential gene analysis resource-poor or environments.

Язык: Английский

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