A One Health approach for the genomic characterization of antibiotic-resistant Campylobacter isolates using Nanopore whole-genome sequencing DOI Creative Commons
Ana Hurtado, Medelin Ocejo,

Beatriz Oporto

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Янв. 29, 2025

In response to the growing threat posed by spread of antimicrobial resistance in zoonotic Campylobacter , a One Health approach was used examine genomic diversity, phylogenomic relationships, and distribution genetic determinants (GDR) C. jejuni coli isolates from humans, animals (ruminants, swine, chickens), avian food products collected during regionally (Basque Country, Spain) temporally (mostly 2021–2022) restricted sampling. Eighty-three seventy-one isolates, most exhibiting ciprofloxacin and/or erythromycin, were whole-genome sequenced using Oxford Nanopore Technologies long-fragment sequencing (ONT). Multilocus sequence typing (MLST) analysis identified high diversity among isolates. Phylogenomic showed that clustering based on core genome aligned with MLST profiles, regardless sample source. contrast, accessory content sometimes discriminated within same STs occasionally differentiated different sources. The majority GDRs present sources, good correlation observed between GDR phenotypic susceptibility profiles (based minimum inhibitory concentrations interpreted according EUCAST epidemiological cutoff values). Genotypic independent genotypes, indicating no apparent association phylogenetic origin. This study demonstrates ONT is powerful tool for molecular surveillance bacterial pathogens framework.

Язык: Английский

A One Health approach for the genomic characterization of antibiotic-resistant Campylobacter isolates using Nanopore whole-genome sequencing DOI Creative Commons
Ana Hurtado, Medelin Ocejo,

Beatriz Oporto

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Янв. 29, 2025

In response to the growing threat posed by spread of antimicrobial resistance in zoonotic Campylobacter , a One Health approach was used examine genomic diversity, phylogenomic relationships, and distribution genetic determinants (GDR) C. jejuni coli isolates from humans, animals (ruminants, swine, chickens), avian food products collected during regionally (Basque Country, Spain) temporally (mostly 2021–2022) restricted sampling. Eighty-three seventy-one isolates, most exhibiting ciprofloxacin and/or erythromycin, were whole-genome sequenced using Oxford Nanopore Technologies long-fragment sequencing (ONT). Multilocus sequence typing (MLST) analysis identified high diversity among isolates. Phylogenomic showed that clustering based on core genome aligned with MLST profiles, regardless sample source. contrast, accessory content sometimes discriminated within same STs occasionally differentiated different sources. The majority GDRs present sources, good correlation observed between GDR phenotypic susceptibility profiles (based minimum inhibitory concentrations interpreted according EUCAST epidemiological cutoff values). Genotypic independent genotypes, indicating no apparent association phylogenetic origin. This study demonstrates ONT is powerful tool for molecular surveillance bacterial pathogens framework.

Язык: Английский

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