Nanoparticle-mediated stimulus-responsive antibacterial therapy

Biomaterials Science, Год журнала: 2023, Номер 11(6), С. 1994 - 2019

Опубликована: Янв. 1, 2023

This work demonstrates different stimulus-based antibacterial treatment strategies for both resistant bacteria and biofilms. Moreover, a future perspective has been illustrated making these modalities clinically feasible.

Язык: Английский

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Unraveling the Microbiome–Human Body Axis: A Comprehensive Examination of Therapeutic Strategies, Interactions and Implications

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5561 - 5561

Опубликована: Май 20, 2024

This review scrutinizes the intricate interplay between microbiome and human body, exploring its multifaceted dimensions far-reaching implications. The microbiome, comprising diverse microbial communities inhabiting various anatomical niches, is increasingly recognized as a critical determinant of health disease. Through an extensive examination current research, this elucidates dynamic interactions host physiology across multiple organ systems. Key topics include establishment maintenance microbiota diversity, influence factors on composition, bidirectional communication pathways cells. Furthermore, we delve into functional implications dysbiosis in disease states, emphasizing role shaping immune responses, metabolic processes, neurological functions. Additionally, discusses emerging therapeutic strategies aimed at modulating to restore host–microbe homeostasis promote health. Microbiota fecal transplantation represents groundbreaking approach management dysbiosis-related diseases, offering promising avenue for restoring balance within gut ecosystem. innovative therapy involves transfer from healthy donor individual suffering dysbiosis, aiming replenish beneficial populations mitigate pathological imbalances. By synthesizing findings fields, offers valuable insights complex relationship highlighting avenues future research clinical interventions.

Язык: Английский

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Swine Gastrointestinal Microbiota and the Effects of Dietary Amino Acids on Its Composition and Metabolism

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 1237 - 1237

Опубликована: Янв. 19, 2024

Many researchers consider gut microbiota (trillions of microorganisms) an endogenous organ its animal host, which confers a vast genetic diversity in providing the host with essential biological functions. Particularly, regulates not only tissue structure but also health and functionality. This paper first summarized those common bacterial species (dominated by Firmicutes, Bacteroidota, Proteobacteria phyla) swine then briefly discussed their roles nutrition health, include nutrient metabolism, pathogen exclusion, immunity modulation. Secondly, current knowledge on how dietary nutrients feed additives affect composition metabolism pigs was discussed. Finally, amino acids relative abundances bacteria reviewed. Tryptophan supplementation promotes growth beneficial suppresses pathogens, while arginine affects nitrogen recycling, impacting immune response health. Glutamate glutamine supplementations elevate levels mitigate pathogenic ones. It concluded that nutritional strategies to manipulate microbial ecosystems are useful measures optimize For example, promote Lactobacillus Bifidobacterium can lead better performance, especially when protein is limited. Further research establish mechanistic cause-and-effect relationships between dynamics will allow producers reap greatest return investment.

Язык: Английский

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In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions

Discover Nano, Год журнала: 2025, Номер 20(1)

Опубликована: Янв. 23, 2025

Diabetic wounds with chronic infections present a significant challenge, exacerbated by the growing issue of antimicrobial resistance, which often leads to delayed healing and increased morbidity. This study introduces novel silver-zinc oxide-eugenol (Ag+ZnO+EU) nanocomposite, specifically designed enhance activity promote wound healing. The nanocomposite was thoroughly characterized using advanced analytical techniques, confirming its nanoscale structure, stability chemical composition. Ag+ZnO+EU demonstrated potent efficacy against range associated pathogens, including standard clinical isolates Staphylococcus aureus, Pseudomonas aeruginosa Candida albicans. Minimum inhibitory concentrations for were significantly lower than those individual components, highlighting synergistic effect nanocomposite. Time-kill assays revealed rapid microbial eradication, achieving complete sterility within 240-min. Importantly, effectively eliminated persister-like cells, are typically resistant conventional treatments, suggesting potential solution persistent infections. In vitro scratch human keratinocyte cells that accelerated closure, near-complete observed 24-h, indicating enhanced cell migration tissue regeneration. Additionally, showed antidiabetic effects increasing glucose uptake up 97.21% in an assay 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose, fluorescent analog, applications beyond These findings highlight as promising candidate addressing both resistance impaired diabetic contexts.

Язык: Английский

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Gut microbiota, host genetics and phenotypes in aquatic animals: A review

Aquaculture Reports, Год журнала: 2023, Номер 31, С. 101648 - 101648

Опубликована: Июль 7, 2023

In aquatic animals, microbial communities associated with the host exert powerful influences on physiology, reproduction, nutrient metabolism, and phenotypic traits, as well complex behaviors. This relationship between microbes suggests that host-microbiota interactions might have influenced host's genetic variation. To improve our understanding of how hosts interact produce phenotypes, it is necessary to focus animal traits a basis understand potential roles for microbiome in shaping phenotypes. Here, we review literatures environmental effects intestinal community structure, corresponding microbiomes phenotypes breeding. provides better gut microbiota establishes connection factors, while modulating affect differentiation. A more detailed contribute determining role field breeding animals.

Язык: Английский

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Study on intestinal microbial communities of three different cattle populations on Qinghai-Tibet Plateau

PLoS ONE, Год журнала: 2025, Номер 20(2), С. e0312314 - e0312314

Опубликована: Фев. 4, 2025

The Tibetan cattle, indispensable·animals on the Qinghai-Tibet Plateau, have become a focal point for region’s economic development. As such, hybridization of these cattle has been recognized as pivotal strategy to enhance local industry. However, research gut microbiota hybrid remains scarce. Based this, we conducted comparative analysis and its functional implications across three distinct populations: two populations (Tibetan × Holstein TH Jersey TJ) one locoal population (BL). Bacteroidetes Firmicutes dominate all at phylum level. In addition, predominant phyla in BL were found be Cyanobacteria, Verrucomicrobiota, Actinobacteria, which may important reasons adaptability high-altitude environment Plateau. Further identified specific biomarkers associated with immune systems including Bacteroidales_RF16, Coriobacterium , Muribaculaceae. contrast, are primarily dominated by Oscillospiraceae Clostridia_UCG_014, TJ mainly Christensenellaceae Gammaproteobacteria. KEGG enrichment revealed that showed significant system, energy metabolism, amino acid metabolism-related pathways compared cattle. Overall, results suggest demonstrate enhanced indicate intestinal different altitudes breeds diverse structures functions. Our study presents new perspective role microbiome enhancement

Язык: Английский

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Impact of Early Weaning on Development of the Swine Gut Microbiome

Microorganisms, Год журнала: 2023, Номер 11(7), С. 1753 - 1753

Опубликована: Июль 5, 2023

Considering that pigs are naturally weaned between 12 and 18 weeks of age, the common practice in modern swine industry weaning as early two four age increases challenges during this transition period. Indeed, young with an immature gut suddenly separated from sow, switched milk to a diet consisting only solid ingredients, subjected new social hierarchy mixing multiple litters. From perspective host development, under these conditions causes regression histological structure well digestive barrier functions. While is main center immunity mature animals, underdeveloped has yet contribute function until seven age. The microbiota or microbiome, essential contributor health nutrition their animal host, undergoes dramatic alterations transition, descriptive review aims present microbial ecology-based on events. communities dependent cross-feeding relationships, change substrate availability triggers cascade succession events stable composition reached. During process, unstable prone dysbiosis, which can devolve into diseased state. One potential strategy accelerate maturation microbiome would be identify species critical microbiomes, develop strategies facilitate establishment post-weaning communities.

Язык: Английский

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A first characterization of the microbiota-resilience link in swine

Microbiome, Год журнала: 2024, Номер 12(1)

Опубликована: Март 15, 2024

Abstract Background The gut microbiome plays a crucial role in understanding complex biological mechanisms, including host resilience to stressors. Investigating the microbiota-resilience link animals and plants holds relevance addressing challenges like adaptation of agricultural species warming environment. This study aims characterize connection swine. As is not directly observable, we estimated it using four distinct indicators based on daily feed consumption variability, assuming with greater intake variation may face maintaining stable physiological status. These were analyzed both as linear categorical variables. In our first set analyses, explored PERMANOVA, α-diversity analysis, discriminant analysis. Additionally, quantified ratio microbiota variance total phenotypic (microbiability). Finally, conducted Partial Least Squares-Discriminant Analysis (PLS-DA) assess classification performance expressed classes. Results offers key insights. Firstly, among all indicators, two effectively captured resilience. Secondly, analyses revealed robust relationship between microbial composition terms richness. We found decreased less-resilient animals, while specific amplicon sequence variants (ASVs) KEGG pathways associated inflammatory responses negatively linked Thirdly, considering classes, observed significant differences primarily lower Lastly, indicates that can serve reliable biomarker for distinguishing individuals Conclusion Our comprehensive have highlighted host-microbiota connection, contributing valuable insights existing scientific knowledge. practical implications PLS-DA microbiability results are noteworthy. suggests interactions could be utilized biomarkers monitoring Furthermore, findings show leveraging improve identification resilient supporting their adaptive capacity response changing environmental conditions. offer promising avenues enhancing animal well-being strategies context faced by livestock populations.

Язык: Английский

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Contribution of trehalose to ethanol stress tolerance of Wickerhamomyces anomalus

BMC Microbiology, Год журнала: 2023, Номер 23(1)

Опубликована: Авг. 29, 2023

Abstract Background The ascomycetous heterothallic yeast Wickerhamomyces anomalus (WA) has received considerable attention and been widely reported in the winemaking industry for its distinctive physiological traits metabolic attributes. An increased concentration of ethanol during fermentation, however, causes stress (ES) on cells. Trehalose implicated improving survival under various conditions microorganisms. Herein, we determined effects trehalose supplementation survival, differentially expressed genes (DEGs), cellular morphology, oxidative tolerance WA response to ES. Results results indicated that improved anomalous surface ultrastructural morphology WA. Additionally, redox homeostasis by reducing levels reactive oxygen species (ROS) inducing activities antioxidant enzymes. In addition, DEGs affected application were enriched these categories including gene expression, protein synthesis, energy metabolism, cell cycle pathways. content intracellular malondialdehyde (MDA) adenosine triphosphate. Conclusions These reveal protective role ES mitigation strengthen possible uses wine fermentation sector.

Язык: Английский

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Comparison of metagenomic analysis of fecal and gastrointestinal tract samples for identifying beneficial gut microorganisms

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 26, 2025

Introduction Previous research on the gut microbiome has primarily focused fecal microbiota, raising concerns about whether samples accurately represent entire intestinal microbiota. Studies have shown that microbial communities across gastrointestinal (GI) tract are more diverse than those in feces, suggesting composition may vary depending sampling method. Additionally, analyzing broader diversity of GI enhance identification potentially beneficial Methods In this study, we compare datasets obtained from and (collected by pooling luminal contents mucosal scrapings stomach to end colon) 6-month-old mice using 16S rRNA sequencing. We further investigate associations between microbiota motor, cognitive, emotional functions mice, examining differences two sample types. To assess these variations, apply DESeq2 analysis identify species enriched high-functioning groups evaluate how their selection differ approach. Results Our findings reveal notable samples, methods influence bacteria. Discussion These results highlight importance selecting an appropriate approach ensure a comprehensive understanding microbiota-host interactions.

Язык: Английский

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