Cell Reports, Год журнала: 2020, Номер 32(6), С. 108007 - 108007
Опубликована: Авг. 1, 2020
Язык: Английский
Nature, Год журнала: 2020, Номер 586(7828), С. 275 - 280
Опубликована: Окт. 7, 2020
Язык: Английский
Процитировано
225
Nature Communications, Год журнала: 2020, Номер 11(1)
Опубликована: Окт. 6, 2020
Abstract Development of high throughput single-cell sequencing technologies has made it cost-effective to profile thousands cells from diverse samples containing multiple cell types. To study how these different types work together, here we develop NATMI (Network Analysis Toolkit for Multicellular Interactions). uses connectomeDB2020 (a database 2293 manually curated ligand-receptor pairs with literature support) predict and visualise cell-to-cell communication networks (or bulk) expression data. Using published datasets demonstrate can be used identify (i) the cell-type that are communicating most specifically) within a network, (ii) active specific) (iii) putative highly-communicating cellular communities (iv) differences in intercellular when profiling given under conditions. Furthermore, analysis Tabula Muris (organism-wide) atlas confirms our previous prediction autocrine signalling is major feature networks, while also revealing hundreds ligands their cognate receptors co-expressed individual suggesting substantial potential self-signalling.
Язык: Английский
Процитировано
203
Nature Cell Biology, Год журнала: 2021, Номер 23(7), С. 745 - 757
Опубликована: Июнь 21, 2021
Язык: Английский
Процитировано
165
World Journal of Gastroenterology, Год журнала: 2023, Номер 29(10), С. 1569 - 1588
Опубликована: Март 14, 2023
Significant progress has been achieved in the treatment of metastatic colorectal cancer (mCRC) patients during last 20 years. There are currently numerous treatments available for first-line mCRC. Sophisticated molecular technologies have developed to reveal novel prognostic and predictive biomarkers CRC. The development next-generation sequencing whole-exome sequencing, which strong new tools discovery facilitate delivery customized treatment, resulted tremendous breakthroughs DNA technology recent appropriate adjuvant mCRC determined by tumor stage, presence high-risk pathologic characteristics, microsatellite instability status, patient age, performance status. Chemotherapy, targeted therapy, immunotherapy main systemic with Despite fact that these choices increased overall survival mCRC, remains optimal individuals non-metastatic disease. being used support our ability practice personalized medicine; practical aspects applying regular clinical practice; evolution chemotherapy, strategies front-line setting all reviewed here.
Язык: Английский
Процитировано
48
Cell stem cell, Год журнала: 2020, Номер 27(4), С. 557 - 573
Опубликована: Сен. 23, 2020
Язык: Английский
Процитировано
107
Advanced Biosystems, Год журнала: 2020, Номер 4(11)
Опубликована: Окт. 9, 2020
Abstract Recent findings about cell fate change induced by physical stimuli have expedited the discovery of underlying regulatory mechanisms that determine stem differentiation. Progress with regards to micro‐/nanofabrication technology led development advanced materials can mimic biophysical features in vivo related circumstances human body. Since cellular microenvironment directly defines structure and function, diverse material properties including stiffness, topology, surface chemistry are investigated regulate multiple signaling cascades involved differentiation for innovative tools be widely utilized various fields ranging from basic research medical applications. This progress report addresses essential regulation alteration applied control It also presents novel strategies based on relationships between recently discovered mechanotransduction pathways signaling. A new perspective physiology will further provide a framework biomedical applications such as regenerative medicine therapy.
Язык: Английский
Процитировано
100
iScience, Год журнала: 2021, Номер 24(5), С. 102405 - 102405
Опубликована: Апрель 23, 2021
Teeth exert fundamental functions related to mastication and speech. Despite their great biomedical importance, an overall picture of cellular molecular composition is still missing. In this study, we have mapped the transcriptional landscape various cell populations that compose human teeth at single-cell resolution, analyzed in deeper detail stem microenvironment. Our study identified heterogeneity dental pulp periodontium. Unexpectedly, found signatures were very similar, while respective microenvironments strongly diverged. findings suggest microenvironmental specificity a potential source for functional differences between highly similar cells located tooth compartments open new perspectives toward cell-based therapeutic approaches.
Язык: Английский
Процитировано
87
Nature Communications, Год журнала: 2021, Номер 12(1)
Опубликована: Дек. 15, 2021
Abstract Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. Here, we employ high-resolution intravital microscopy using a CSC biosensor to directly observe in live mice with mammary tumors. display slow-migratory, invadopod-rich phenotype that is hallmark disseminating tumor cells. enriched near macrophages, particularly macrophage-containing intravasation sites called Tumor Microenvironment Metastasis (TMEM) doorways. Substantial enrichment occurs on association TMEM doorways, contributing finding represent >60% circulating Mechanistically, stemness induced non-stem cancer upon their direct contact macrophages via Notch-Jagged signaling. In breast cancers from patients, density doorways correlates proportion expressing cell markers, indicating human only portals also programming sites.
Язык: Английский
Процитировано
82
Cell Metabolism, Год журнала: 2020, Номер 32(4), С. 629 - 642.e8
Опубликована: Сен. 8, 2020
Язык: Английский
Процитировано
81
PLoS Biology, Год журнала: 2022, Номер 20(1), С. e3001532 - e3001532
Опубликована: Янв. 27, 2022
Chronic inflammation is often associated with the development of tissue fibrosis, but how mesenchymal cell responses dictate pathological fibrosis versus resolution and healing remains unclear. Defining stromal heterogeneity identifying molecular circuits driving extracellular matrix deposition remodeling stands to illuminate relationship between inflammation, healing. We performed single-cell RNA-sequencing colon-derived cells identified distinct classes fibroblasts gene signatures that are differentially regulated by chronic including IL-11–producing inflammatory fibroblasts. further identify a transcriptional program trans -differentiation mucosa-associated define functional signature in inflamed colon. Our analysis supports critical role for metalloprotease Adamdec1 at interface during colitis, demonstrating its requirement colon epithelial integrity. These findings provide mechanistic insight into perturbs behaviors drive fibroblastic controlling mucosal
Язык: Английский
Процитировано
68