EventLFM: event camera integrated Fourier light field microscopy for ultrafast 3D imaging
Light Science & Applications,
Год журнала:
2024,
Номер
13(1)
Опубликована: Июнь 26, 2024
Abstract
Ultrafast
3D
imaging
is
indispensable
for
visualizing
complex
and
dynamic
biological
processes.
Conventional
scanning-based
techniques
necessitate
an
inherent
trade-off
between
acquisition
speed
space-bandwidth
product
(SBP).
Emerging
single-shot
wide-field
offer
a
promising
alternative
but
are
bottlenecked
by
the
synchronous
readout
constraints
of
conventional
CMOS
systems,
thus
restricting
data
throughput
to
maintain
high
SBP
at
limited
frame
rates.
To
address
this,
we
introduce
EventLFM,
straightforward
cost-effective
system
that
overcomes
these
challenges
integrating
event
camera
with
Fourier
light
field
microscopy
(LFM),
state-of-the-art
technique.
The
operates
on
novel
asynchronous
architecture,
thereby
bypassing
rate
limitations
systems.
We
further
develop
simple
robust
event-driven
LFM
reconstruction
algorithm
can
reliably
reconstruct
dynamics
from
unique
spatiotemporal
measurements
captured
EventLFM.
Experimental
results
demonstrate
EventLFM
robustly
fast-moving
rapidly
blinking
fluorescent
samples
kHz
Furthermore,
highlight
EventLFM’s
capability
neuronal
signals
in
scattering
mouse
brain
tissues
tracking
GFP-labeled
neurons
freely
moving
C.
elegans
.
believe
combined
ultrafast
large
offered
may
open
up
new
possibilities
across
many
biomedical
applications.
Язык: Английский
Lensless imaging with a programmable Fresnel zone aperture
Science Advances,
Год журнала:
2025,
Номер
11(12)
Опубликована: Март 21, 2025
Optical
imaging
has
long
been
dominated
by
traditional
lens-based
systems
that,
despite
their
success,
are
inherently
limited
size,
weight,
and
cost.
Lensless
seeks
to
overcome
these
limitations
replacing
lenses
with
thinner,
lighter,
cheaper
optical
modulators
reconstructing
images
computationally,
while
facing
trade-offs
in
image
quality,
artifacts,
flexibility
inherent
static
modulation.
Here,
we
propose
a
lensless
method
programmable
Fresnel
zone
aperture
(FZA),
termed
LIP.
With
commercial
liquid
crystal
display,
designed
an
integrated
LIP
module
demonstrated
its
capability
of
high-quality
artifact-free
reconstruction
through
dynamic
modulation
offset-FZA
parallel
merging.
Compared
static-modulation
approaches,
achieves
2.5×
resolution
enhancement
3
decibels
improvement
signal-to-noise
ratio
“static
mode”
maintaining
interaction
frame
rate
15
frames
per
second
“dynamic
mode.”
Experimental
results
demonstrate
LIP’s
potential
as
miniaturized
platform
for
versatile
advanced
tasks
like
virtual
reality
human-computer
interaction.
Язык: Английский
V-shaped PSF for 3D imaging over an extended depth of field in wide-field microscopy
Optics Letters,
Год журнала:
2024,
Номер
50(2), С. 383 - 383
Опубликована: Дек. 2, 2024
Single-shot
3D
optical
microscopy
that
can
capture
high-resolution
information
over
a
large
volume
has
broad
applications
in
biology.
Existing
imaging
methods
using
point-spread-function
(PSF)
engineering
often
have
limited
depth
of
field
(DOF)
or
require
custom
and
complex
design
phase
masks.
We
propose
new,
to
the
best
our
knowledge,
PSF
approach
is
easy
implement
offers
DOF.
The
appears
be
axially
V-shaped,
engineered
by
replacing
conventional
tube
lens
with
pair
axicon
lenses
behind
objective
wide-field
microscope.
reconstructed
from
single-shot
image
deep
neural
network.
Simulations
10×
magnification
microscope
show
V-shaped
excellent
resolution
(<2.5
µm
lateral
∼15
axial)
∼350
DOF
at
550
nm
wavelength.
Compared
other
popular
PSFs
designed
for
imaging,
simple
deploy
provides
high
reconstruction
quality
an
extended
Язык: Английский