bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Авг. 12, 2022
Abstract
Epithelial
cells
remodel
cell
adhesion
and
change
their
neighbors
to
shape
a
tissue.
This
rearrangement
proceeds
in
three
steps:
the
shrinkage
of
junction,
exchange
junctions,
elongation
newly
generated
junction.
Herein,
by
combining
live
imaging
physical
modeling,
we
showed
that
formation
myosin-II
(myo-II)
cables
around
vertices
underlies
junctions.
The
local
transient
detachment
myo-II
from
cortex
is
coupled
with
junction
via
an
interplay
between
LIM
domain-containing
protein
Jub
tricellular
M6.
Furthermore,
developed
mechanical
model
based
on
wetting
theory
clarified
way
which
properties
are
integrated
geometry
induce
transition
attached
detached
states
support
unidirectionality
rearrangement.
Collectively,
present
study
elucidates
orchestration
geometry,
mechanics
signaling
for
exchanging
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(13)
Опубликована: Март 22, 2023
Biological
tissues
acquire
reproducible
shapes
during
development
through
dynamic
cell
behaviors.
Most
of
these
behaviors
involve
the
remodeling
cell–cell
contacts.
During
epithelial
morphogenesis,
contractile
actomyosin
networks
remodel
contacts
by
shrinking
and
extending
junctions
between
lateral
surfaces.
However,
not
only
generate
mechanical
stresses
but
also
respond
to
them,
confounding
our
understanding
how
Here,
we
develop
a
two-point
optical
manipulation
method
impose
different
stress
patterns
on
in
early
epithelium
Drosophila
embryo.
The
technique
allows
us
produce
junction
extension
shrinkage
push
pull
manipulations
at
edges
junctions.
We
use
observations
expand
classical
vertex-based
models
tissue
mechanics,
incorporating
negative
positive
mechanosensitive
feedback
depending
type
remodeling.
In
particular,
show
that
Myosin-II
activity
responds
strain
rate
facilitates
full
shrinkage.
Altogether
work
provides
insight
into
produces
efficient
deformation
vivo
identifies
unanticipated
features
their
PLoS Computational Biology,
Год журнала:
2022,
Номер
18(12), С. e1010762 - e1010762
Опубликована: Дек. 16, 2022
We
introduce
a
modelling
and
simulation
framework
for
cell
aggregates
in
three
dimensions
based
on
interacting
active
surfaces.
Cell
mechanics
is
captured
by
physical
description
of
the
acto-myosin
cortex
that
includes
cortical
flows,
viscous
forces,
tensions,
bending
moments.
Cells
interact
with
each
other
via
short-range
forces
capturing
effect
adhesion
molecules.
discretise
model
equations
using
finite
element
method,
provide
parallel
implementation
C++.
discuss
examples
application
this
to
small
medium-sized
aggregates:
we
consider
shape
dynamics
doublet,
planar
sheet,
growing
aggregate.
This
opens
door
systematic
exploration
tissue-scale
aggregates,
which
plays
key
role
morphogenesis
embryos
organoids.
PLoS Computational Biology,
Год журнала:
2024,
Номер
20(4), С. e1012001 - e1012001
Опубликована: Апрель 1, 2024
Epithelial
tissues
are
the
most
abundant
tissue
type
in
animals,
lining
body
cavities
and
generating
compartment
barriers.
The
function
of
a
monolayered
epithelial
tissue–whether
protective,
secretory,
absorptive,
or
filtrative–relies
on
side-by-side
arrangement
its
component
cells.
mechanical
parameters
that
determine
shape
cells
apical-basal
plane
not
well-understood.
architecture
culture
is
intimately
connected
to
cell
density,
cultured
layers
transition
between
architectures
as
they
proliferate.
This
prompted
us
ask
what
extent
emerges
from
two
considerations:
A)
constraints
densification
B)
cell-cell
adhesion,
hallmark
feature
To
address
these
questions,
we
developed
novel
polyline
cell-based
computational
model
used
it
make
theoretical
predictions
about
upon
changes
density
adhesion.
We
tested
using
experiments.
Our
results
show
appearance
extended
lateral
borders
arises
consequence
crowding–independent
However,
cadherin-mediated
adhesion
associated
with
architectural
transition.
suggest
this
represents
initial
distinctive
architecture.
Together
our
work
reveals
distinct
roles
layer
formation
provides
framework
understand
less
well-studied
tissues.
PLoS Biology,
Год журнала:
2024,
Номер
22(4), С. e3002611 - e3002611
Опубликована: Апрель 29, 2024
As
tissues
grow
and
change
shape
during
animal
development,
they
physically
pull
push
on
each
other,
these
mechanical
interactions
can
be
important
for
morphogenesis.
During
Drosophila
gastrulation,
mesoderm
invagination
temporally
overlaps
with
the
convergence
extension
of
ectodermal
germband;
latter
is
caused
primarily
by
Myosin
II–driven
polarised
cell
intercalation.
Here,
we
investigate
impact
ectoderm
extension,
examining
possible
mechanotransductive
effects
II
recruitment
We
find
that
germband
deformed
pulling
in
orthogonal
direction
to
(GBE),
showing
coupling
between
tissues.
However,
do
not
a
significant
planar
polarisation
response
invagination,
nor
rate
junction
shrinkage
leading
neighbour
exchange
events.
conclude
main
cellular
mechanism
axis
intercalation,
robust
pull.
find,
however,
slows
down
anterior-posterior
elongation
contributes
counteracting
tension
from
endoderm
which
pulls
along
GBE.
PLoS Computational Biology,
Год журнала:
2025,
Номер
21(5), С. e1012993 - e1012993
Опубликована: Май 21, 2025
Vertex
models
provide
a
robust
theoretical
framework
for
studying
epithelial
tissues
as
network
of
cell
boundaries.
They
have
been
pivotal
in
exploring
properties
such
packing
geometry
and
rigidity
transitions.
Recently,
extended
vertex
become
instrumental
bridging
the
subcellular
scales
to
tissue
scale.
Here,
we
review
extensions
model
aiming
capture
experimentally
observed
features
including
heterogeneity
myosin
activity
across
tissue,
non-uniform
contractility
structures,
mechanosensitive
feedback
loops.
We
discuss
how
these
change
challenge
current
perspectives
on
observables
macroscopic
properties.
First,
find
that
can
significantly,
impacting
critical
threshold
some
cases
even
existence
transition.
Second,
disorder
be
explained
by
employing
different
mechanisms,
indicating
source
stochasticity
gradual
local
size
changes
common
mesoscopic
motifs
mechanics
organization.
address
complementary
statistical
inference,
putting
broader
methodological
context
give
brief
overview
software
packages
utilized
increasingly
complex
studies.
Our
emphasizes
need
more
comparative,
systematic
studies
identify
specific
classes
which
share
set
well-defined
properties,
well
in-depth
discussion
modeling
choices
their
biological
motivations.
Current Opinion in Cell Biology,
Год журнала:
2024,
Номер
91, С. 102427 - 102427
Опубликована: Сен. 26, 2024
Epithelial
cells
adhere
to
each
other
via
intercellular
junctions
that
can
be
classified
into
bicellular
and
tricellular
contacts
(vertices).
morphogenesis
involves
cell
rearrangement
requires
remodeling
of
vertices.
Although
our
understanding
how
junction
mechanics
drive
epithelial
has
advanced,
the
mechanisms
underlying
vertex
during
this
process
have
only
received
attention
recently.
In
review,
we
outline
recent
progress
in
reorganize
adhesion
cytoskeleton
trigger
displacement
resolution
We
will
also
discuss
achieve
optimal
balance
between
structural
flexibility
stability
their
Finally,
introduce
new
modeling
frameworks
designed
analyze
at
Integration
live
imaging
techniques
is
providing
insights
active
roles
vertices
morphogenesis.
