Laboratory Investigation, Год журнала: 2024, Номер 104(12), С. 102150 - 102150
Опубликована: Окт. 10, 2024
Язык: Английский
International Journal of Cancer, Год журнала: 2025, Номер unknown
Опубликована: Апрель 25, 2025
Abstract Micropeptides are commonly identified as peptides encoded by non‐coding RNAs (ncRNAs). In the short open reading frame (sORF) of ncRNAs, there is a base sequence encoding functional micropeptides, which great significance in biological field. Recently, micropeptides regulate diverse processes, including mitochondrial metabolism, calcium transport, mRNA splicing, signal transduction, myocyte fusion, and cellular senescence, regulating homeostasis internal environment cancer's incidence progression. Especially, study cancer about potential regulatory mechanism will be conducive to further understanding process initiation development. More more research shows have been confirmed play an essential role emergence multiple kinds cancers, Breast cancer, Colon Colorectal Glioma, Glioblastoma, Liver cancer. This review presents comprehensive synthesis latest advancements our roles cells, with particular focus on networks involving oncogenesis. The new mode action provides innovative ideas for diagnosis treatment. Moreover, we explored significant capacity diagnostic biomarkers targets anti‐cancer therapies clinical settings, highlighting their development micropeptide‐based tools therapeutics.
Язык: Английский
Процитировано
0
Cancers, Год журнала: 2025, Номер 17(9), С. 1555 - 1555
Опубликована: Май 3, 2025
Non-coding RNAs (ncRNAs) constitute the majority of human transcriptome and play diverse structural, catalytic, regulatory roles. The ability ncRNAs to be translated into functional peptides microproteins expands our understanding their potential beyond established non-coding functions. Our comprehensive search identified 86 translating “non-coding” RNAs. While have traditionally been categorized as “peptide-encoding”, in this study, we introduce a novel classification based on amino acid length, distinguishing products ncRNA encoded (ncRNA-PEPs), which are less than 60 acids, or (ncRNA-MPs) ranging from 61 200 acids. These act co-regulators cell signaling, transcriptional regulation, protein complex assembly, playing role both health disease. We outline molecular pathways by ncRNA-PEPs ncRNA-MPs could govern cycle progression, highlighting influence transitions, oncogenic tumor suppressor pathways, metabolic homeostasis, autophagy, key regulators like PCNA, Rad18, CDK–cyclin complexes. Furthermore, highlight recent advancements detection characterization, exploring evolutionary origins, species-specific conservation, therapeutic applications. findings underscore emerging significance integral cellular processes, versatility opening promising avenues for further research
Язык: Английский
Процитировано
0
International Journal of COPD, Год журнала: 2025, Номер Volume 20, С. 1543 - 1553
Опубликована: Май 1, 2025
Chronic obstructive pulmonary disease (COPD) primarily results from cigarette smoke (CS)-induced chronic inflammation. Although numerous long non-coding ribonucleic acids (lncRNAs) have been extensively studied for their crucial roles in COPD, the peptides encoded by these lncRNAs garnered limited attention. This study aimed to investigate role of a peptide lncRNA HOXB-AS3 extract (CSE)-induced inflammation and 16HBE cells. Open reading frames (ORF) Find software was utilized predict encoding potential HOXB-AS3. Quantitative real-time polymerase chain reaction (qRT-PCR) employed detect levels HOXB-AS3-32aa peripheral blood mononuclear cells (PBMCs) both healthy controls COPD patients exposed different CSE. To establish an vitro inflammatory cell model were treated with 2% Enzyme-Linked Immunosorbent Assay (ELISA) measured cytokines, while CCK-8 assay assessed viability. Flow cytometry assess apoptosis. Western blot analysis performed measure expression HOXB-AS3-32aa, EZH2, H3K27me3 proteins. Co-Immunoprecipitation (Co-IP) conducted verify interaction between EZH2 HOXB-AS3-32aa. Our findings revealed elevated PBMCs compared controls. CSE treatment dose-dependently increased expression. Overexpression exacerbated CS-induced bronchial epithelial cells, leading inhibited proliferation Furthermore, suppressed H3k27me3 protein Co-IP confirmed protein. demonstrate that novel promotes apoptosis via EZH2-mediated modification.
Язык: Английский
Процитировано
0
Frontiers in Oncology, Год журнала: 2024, Номер 14
Опубликована: Июль 4, 2024
CRC poses a significant challenge in the global health domain, with high number of deaths attributed to this disease annually. If is detected only its advanced stages, difficulty treatment increases significantly. Therefore, biomarkers for early detection play crucial role improving patient outcomes and increasing survival rates. The development reliable biomarker particularly important timely diagnosis treatment. However, current methods detection, such as endoscopic examination, blood, stool tests, have certain limitations often detect cases late stages. To overcome these constraints, researchers turned their attention molecular biomarkers, which are considered promising approach detection. Non-invasive using mRNA, circulating cell-free DNA, microRNA, LncRNA, proteins can provide more diagnostic information. These be found tissue, stool, volatile organic compounds. Identifying sensitivity specificity safe, economic, easily measurable remains researchers.
Язык: Английский
Процитировано
3
The FASEB Journal, Год журнала: 2023, Номер 37(12)
Опубликована: Ноя. 23, 2023
Abstract Rapid advances in high‐quality sequencing and bioinformatics have invalidated the argument that noncoding RNAs (ncRNAs) are junk transcripts do not encode proteins. Increasing evidence suggests small open reading frames (sORFs) ncRNAs can micropeptides polypeptides within 100 amino acids length. Several been characterized proven to various functions human physiology pathology, particularly cancer. The present review mainly highlights latest studies on ncRNA‐encoded different cancers categorizes them based their subcellular localization, thereby providing a theoretical basis for micropeptide applications early diagnosis prognosis of cancer as therapeutic targets. However, considering inherent characteristics limitations assay technology methods, more detailed information is warranted.
Язык: Английский
Процитировано
6
Poultry Science, Год журнала: 2024, Номер 103(5), С. 103582 - 103582
Опубликована: Фев. 22, 2024
Small peptides are nutrients and bioactive molecules that have dual regulatory effects on nutrition physiology. They of great significance for maintaining the intestinal health production performance broilers. We here cultured primary small epithelial cells (IEC) chicken in a medium containing L-Leu (Leu) L-Leu-L-Leu (Leu-Leu) 24 h. The untreated were considered as control group. growth, proliferation, apoptosis IEC examined. By combining RNA-seq label-free sequencing technology, candidate genes, proteins, pathways related to screened. Immunofluorescence detection revealed purity isolated was >90%. Leu-Leu group significantly promoted growth proliferation inhibited apoptosis, effect better than those Leu groups. Using transcriptome sequencing, four CCL20, IL8L1, IL8, IL6, screened group, one gene, Two IL6 RGN, compared with Nonquantitative proteomic marker results through screening proteins pathways, found growth-related protein PGM3 three proliferation-related RPS17, RPS11, RPL23, two apoptosis-related GPX4 PDPK1 In short, could promote inhibit apoptosis. On proteome technologies, multiple immune- energy-related signal be Three genes RGN identified, six PGM3, GPX4, involved provide valuable data preliminarily elucidating peptide-mediated regulation improving peptide theoretical system, establishing technology.
Язык: Английский
Процитировано
2
Опубликована: Июнь 28, 2024
Cancer develops from abnormal cell growth in the body, causing significant mortalities every year. To date, potent therapeutic approaches have been developed to eradicate tumor cells, but intolerable toxicity and drug resistance can occur treated patients, limiting efficiency of existing treatment strategies. Therefore, searching for novel genes critical cancer progression response is urgently needed successful therapy. Recent advances bioinformatic proteomic techniques allowed identification a category peptides encoded by non-canonical open reading frames (ncORFs) historically non-coding genomic regions. Surprisingly, many ncORFs express functional microproteins that play vital role human cancers. In this review, we provide comprehensive description different ncORF types with coding capacity technological methods discovering among genomes. We also summarize diagnostic prognostic value these as well their carcinogenic underestimated potential targets.
Язык: Английский
Процитировано
2
Cancers, Год журнала: 2024, Номер 16(15), С. 2660 - 2660
Опубликована: Июль 26, 2024
Cancer develops from abnormal cell growth in the body, causing significant mortalities every year. To date, potent therapeutic approaches have been developed to eradicate tumor cells, but intolerable toxicity and drug resistance can occur treated patients, limiting efficiency of existing treatment strategies. Therefore, searching for novel genes critical cancer progression response is urgently needed successful therapy. Recent advances bioinformatics proteomic techniques allowed identification a category peptides encoded by non-canonical open reading frames (ncORFs) historically non-coding genomic regions. Surprisingly, many ncORFs express functional microproteins that play vital role human cancers. In this review, we provide comprehensive description different ncORF types with coding capacity technological methods discovering among genomes. We also summarize carcinogenic such as pTINCR HOXB-AS3 regulating hallmarks cancer, well roles CIP2A-BP diagnosis prognosis. discuss how AKT-174aa DDUP are involved anti-cancer underestimated potential targets.
Язык: Английский
Процитировано
2
Current Proteomics, Год журнала: 2024, Номер 21(3), С. 140 - 161
Опубликована: Авг. 15, 2024
: Transposable elements are the oldest structural and functional units that were formed during emergence of life on Earth. The most ancient properties transposable multifunctionality their transcription translation products formation many variants through processing, due to which key evolutionary sources long non-coding RNAs, circular microRNAs, proteins peptides formation. Moreover, same type transposon can simultaneously serve as source origin all these molecules, providing adaptive living organisms, especially complex eukaryotes, including humans. ability for mutual integration protein interacting with DNA RNA well regulation functionality RNA, is basis RNAs characterized by properties. This explain factors from elements, is, capable structures molecules presence specific amino acid sequences derived elements. article presents facts about evolution non-- coding genes Specific translated pri-microRNAs described, reflect in evolution. These promising tools treatment viral infections drug-resistant tumors, since, together they involved antiviral antitumor responses.
Язык: Английский
Процитировано
2
Frontiers in Pharmacology, Год журнала: 2024, Номер 15
Опубликована: Сен. 16, 2024
Non-coding RNAs (ncRNAs), which are usually considered not to encode proteins, widely involved in important activities including signal transduction and cell proliferation. However, recent studies have shown that small peptides encoded by ncRNAs (SPENs) roles the development of malignant tumors. Some SPENs participate regulation skeleton reorganization, intercellular adhesion, signaling other processes tumor cells, with effects on invasive migratory abilities cells. Therefore, potential applications as therapeutic targets biomarkers Invasion migration cells main reasons for poor prognosis cancer patients represent most challenging aspects treatment Currently, treatments tumors include surgery, radiotherapy, targeted drug therapy. Surgery, however, is reserved early stages carries risks costs. Radiotherapy therapy serious side effects. This review describes mechanisms their invasion migration, aim providing new diagnosis treatment.
Язык: Английский
Процитировано
2