Asymmetric neuromodulation in the respiratory network contributes to rhythm and pattern generation DOI Creative Commons
Rishi R. Dhingra, Peter M. MacFarlane, Peter J. Thomas

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 11, 2024

Like other brain circuits, the brainstem respiratory network is continually modulated by neurotransmitters that activate slow metabotropic receptors. In many cases, activation of these receptors only subtly modulates motor pattern. However, some receptor types evokes arrest pattern as can occur following µ-opioid We propose varied effects neuromodulation on depend neuromodulator expression and their influence excitability post-synaptic targets. Because a comprehensive characterization cellular properties across remains challenging, we test our hypothesis combining computational modelling with ensemble electrophysiologic recording in pre-Bötzinger complex (pre-BötC) using high-density multi-electrode arrays (MEA). Our model encapsulates neuromodulatory transmission organized asymmetrically to promote rhythm generation. To this hypothesis, increased strength connections used selective agonists

Язык: Английский

Inspiratory and sigh breathing rhythms depend on distinct cellular signalling mechanisms in the preBötzinger complex DOI Creative Commons
Daniel S. Borrus,

Marco K. Stettler,

Cameron J. Grover

и другие.

The Journal of Physiology, Год журнала: 2024, Номер 602(5), С. 809 - 834

Опубликована: Фев. 14, 2024

Abstract Breathing behaviour involves the generation of normal breaths (eupnoea) on a timescale seconds and sigh order minutes. Both rhythms emerge in tandem from single brainstem site, but whether how cell population can generate two disparate remains unclear. We posit that recurrent synaptic excitation concert with depression cellular refractoriness gives rise to eupnoea rhythm, whereas an intracellular calcium oscillation is slower by orders magnitude rhythm. A mathematical model capturing these dynamics simultaneously generates frequencies, which be separately regulated physiological parameters. experimentally validated key predictions regarding signalling. All vertebrate brains feature network oscillator drives breathing pump for regular respiration. However, air‐breathing mammals compliant lungs susceptible collapse, rhythmogenic may have refashioned ubiquitous signalling systems produce second rhythm (for sighs) prevents atelectasis without impeding eupnoea. image Key points simplified activity‐based preBötC inspiratory neuron population. Inspiration attributable canonical excitatory mechanism. Sigh emerges The predicts perturbations uptake release across endoplasmic reticulum counterintuitively accelerate decelerate rhythmicity, respectively, was validated. Vertebrate evolution adapted existing mechanisms slow oscillations needed optimize pulmonary function mammals.

Язык: Английский

Процитировано

7
Degenerate Neuronal and Circuit Mechanisms Important for Generating Rhythmic Motor Patterns DOI
Ronald L. Calabrese, Eve Marder

Physiological Reviews, Год журнала: 2024, Номер 105(1), С. 95 - 135

Опубликована: Июнь 13, 2024

In 1996, we published a review article (Marder E, Calabrese RL.

Язык: Английский

Процитировано

4
Putting the theory into ‘burstlet theory’ with a biophysical model of burstlets and bursts in the respiratory preBötzinger complex DOI Creative Commons
Ryan S. Phillips, Jonathan Rubin

eLife, Год журнала: 2022, Номер 11

Опубликована: Апрель 5, 2022

Inspiratory breathing rhythms arise from synchronized neuronal activity in a bilaterally distributed brainstem structure known as the preBötzinger complex (preBötC). In vitro slice preparations containing preBötC, extracellular potassium must be elevated above physiological levels (to 7–9 mM) to observe regular rhythmic respiratory motor output hypoglossal nerve which preBötC projects. Reexamination of how K + affects has revealed that low-amplitude oscillations persist at levels. These oscillatory events are subthreshold standpoint transmission and dubbed burstlets. Burstlets neural rhythmogenic subpopulation within some instances may fail recruit larger network events, or bursts, required generate output. The fraction (burstlet fraction) decreases sigmoidally with increasing potassium. observations underlie burstlet theory rhythm generation. Experimental computational studies have suggested recruitment non-rhythmogenic component population requires intracellular Ca 2+ dynamics activation calcium-activated nonselective cationic current. this study, we show calcium driven by synaptically triggered influx well release/uptake endoplasmic reticulum conjunction current can reproduce offer an explanation for many key properties associated Altogether, our modeling work provides mechanistic basis unify wide range experimental findings on generation preBötC.

Язык: Английский

Процитировано

18
Analytical strength-duration curve for the spiking response of the LIF neuron to an alpha-function-shaped excitatory current pulse DOI Creative Commons
A. V. Paraskevov

Results in Applied Mathematics, Год журнала: 2025, Номер 25, С. 100548 - 100548

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Symmetric and asymmetric bursting oscillations in a hybrid van der Pol-Duffing-Rayleigh system DOI
Xindong Ma, Zhao Zhang

Chaos Solitons & Fractals, Год журнала: 2024, Номер 186, С. 115310 - 115310

Опубликована: Июль 26, 2024

Язык: Английский

Процитировано

3
Jaynes machine: The universal microstructure of deep neural networks DOI Creative Commons
Venkat Venkatasubramanian,

N. Sanjeevrajan,

Manasi Khandekar

и другие.

Computers & Chemical Engineering, Год журнала: 2024, Номер 192, С. 108908 - 108908

Опубликована: Ноя. 4, 2024

Язык: Английский

Процитировано

3
Transcriptomes of electrophysiologically recorded Dbx1-derived respiratory neurons of the preBötzinger complex in neonatal mice DOI Creative Commons
Prajkta Kallurkar, Maria Cristina D. Picardo, Yae K. Sugimura

и другие.

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Фев. 21, 2022

Breathing depends on interneurons in the preBötzinger complex (preBötC) derived from Dbx1-expressing precursors. Here we investigate whether rhythm- and pattern-generating functions reside discrete classes of Dbx1 preBötC neurons. In a slice model breathing with ~ 5 s cycle period, putatively rhythmogenic Type-1 neurons activate 100-300 ms prior to Type-2 neurons, specialized for output pattern, 300-500 inspiratory motor output. We sequenced transcriptomes identified differential expression 123 genes including ionotropic receptors (Gria3, Gabra1) that may explain their preinspiratory activation profiles Ca

Язык: Английский

Процитировано

11
Inhibitory subpopulations in preBötzinger Complex play distinct roles in modulating inspiratory rhythm and pattern DOI Open Access

Zheng Chang,

Jordan Skach,

Kaiwen Kam

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 8, 2023

Abstract Inhibitory neurons embedded within mammalian neural circuits shape breathing, walking, chewing, and other rhythmic motor behaviors. At the core of circuit controlling breathing is preBötzinger Complex (preBötC), a nucleus in ventrolateral medulla necessary for generation inspiratory rhythm. In preBötC, recurrently connected network glutamatergic Dbx1-derived (Dbx1 + ) generates drive. Functionally anatomically intercalated among preBötC Dbx1 are GABAergic (GAD1/2 glycinergic (GlyT2 neurons, whose roles remain unclear. To elucidate inhibitory microcircuits we first characterized spatial distribution molecularly-defined subpopulations double reporter mice expressing either red fluorescent protein tdTomato or EGFP GlyT2 , GAD1 GAD2 neurons. We found that, neonatal mice, majority expressed combination while much smaller subpopulation also GAD1. determine functional role these subpopulations, used holographic photostimulation, patterned illumination technique with high spatiotemporal resolution, rhythmically active medullary slices from ;GlyT2 ;GAD1 mice. Stimulation 4 8 during endogenous rhythm prolonged interburst interval phase-dependent manner increased latency to burst initiation when bursts were evoked by stimulation contrast, did not affect initiation. Instead, photoactivation duration decreased amplitude. conclude that express both modulate delaying shapes patterning altering

Язык: Английский

Процитировано

6
Inhibitory subpopulations in preBötzinger Complex play distinct roles in modulating inspiratory rhythm and pattern DOI

Zheng Chang,

Jordan Skach,

Kaiwen Kam

и другие.

Journal of Neuroscience, Год журнала: 2024, Номер 44(25), С. e1928232024 - e1928232024

Опубликована: Май 10, 2024

Inhibitory neurons embedded within mammalian neural circuits shape breathing, walking, and other rhythmic motor behaviors. At the core of circuit controlling breathing is preBtzinger Complex (preBtC), where GABAergic (GAD1/2 + ) glycinergic (GlyT2 are functionally anatomically intercalated among glutamatergic Dbx1-derived (Dbx1 that generate inspiratory drive. The roles these preBtC inhibitory in remain unclear. We first characterized spatial distribution molecularly defined subpopulations male female neonatal double reporter mice expressing either tdTomato or EGFP GlyT2 , GAD1 GAD2 neurons. found majority expressed both while a much smaller subpopulation also GAD1. To determine functional role subpopulations, we used holographic photostimulation, patterned illumination technique, rhythmically active medullary slices from Dbx1 ;GlyT2 ;GAD1 sex. Stimulation 4 8 during endogenous rhythm prolonged interburst interval phase-dependent manner increased latency to burst initiation when bursts were evoked by stimulation In contrast, did not affect initiation. Instead, photoactivation duration decreased amplitude. conclude /GAD2 modulate delaying shapes patterning altering

Язык: Английский

Процитировано

1
Analytical strength-duration curve for the spiking response of the LIF neuron to an alpha-function-shaped excitatory current pulse DOI Open Access
A. V. Paraskevov

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 5, 2023

Abstract Whether or not the neuron emits a spike in response to stimulation by an excitatory current pulse is determined strength-duration curve (SDC) for parameters. The SDC dependence of minimal amplitude required elicit spiking on either duration its decay time. Excitatory neurons affect others through pulses postsynaptic current. A simple yet plausible approximation time course such alpha function, with linear rise at start and exponential end. However, exact analytical this case hitherto known, even leaky integrate- and-fire (LIF) neuron, simplest model used practice. We have obtained general equations LIF neuron. Using Lambert W function — widely-implemented special we found stimulated form function. To compare results unified way, also derived SDCs (i) rectangular pulse, (ii) ascending ramp (iii) instantly rising exponentially decaying pulse. In limit no leakage, show that reduced classical hyperbola all considered cases.

Язык: Английский

Процитировано

2