High Dose Metformin Treatment to Inhibit Complex I during Early Reperfusion Protects the Aged Mouse Heart via Decreased MPTP Opening DOI
Qun Chen, Jeremy Thompson, Ying Hu

и другие.

Journal of Pharmacology and Experimental Therapeutics, Год журнала: 2024, Номер 392(3), С. 100529 - 100529

Опубликована: Дек. 24, 2024

Acute, high-dose metformin (MET, 2 mM) results in partial complex I inhibition ischemia (ISC)-modified mitochondria. Mitochondrial permeability transition pore (MPTP) opening increases cardiac injury during ISC-reperfusion (REP). We evaluated whether MET (2 can decrease MPTP aged hearts REP. Sestrin2 (Sesn2) regulates metabolism through activation of AMP-dependent protein kinase. Sesn2 decreases hearts. The knockout (KO) mimics the aging phenotype. Inactivation glycogen synthase kinase-3 β (GSK-3β) via serine-9 phosphorylation opening. assessed if mM given early REP by blockade with decreased and protection depends on Sesn2-mediated GSK-3β phosphorylation. C57BL/6BJ male mice (22-24 months) adult KO were evaluated. dose-dependently inhibited NADH oxidase activity permeabilized mitochondria both greater after 25 minutes ISC. infarct size improved calcium retention capacity wild-type mice. treatment only increased heart but not Thus, at partially inhibits susceptibility downstream is fully dependent inhibition. Complex downregulation acute, has translational potential to protect heart. SIGNIFICANCE STATEMENT: This study explores efficacy mechanism acute reducing mitochondrial-driven reperfusion stop-flow high-risk Metformin (NADH oxidation) ischemia-altered mitigated as injury. modulation application mitigate ST-elevation myocardial infarction

Язык: Английский

Elamipretide: A Review of Its Structure, Mechanism of Action, and Therapeutic Potential DOI Open Access

Cheryl Tung,

Fahimeh Varzideh,

Emanuele Farroni

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 944 - 944

Опубликована: Янв. 23, 2025

Mitochondria serve an essential metabolic and energetic role in cellular activity, their dysfunction has been implicated a wide range of disorders, including cardiovascular conditions, neurodegenerative syndromes. Mitochondria-targeted therapies, such as Elamipretide (SS-31, MTP-131, Bendavia), have consequently emerged topic scientific clinical interest. unique structure allowing for uptake variety cell types highly selective mitochondrial targeting. This mitochondria-targeting tetrapeptide selectively binds cardiolipin (CL), lipid found the inner membrane, thus stabilizing cristae structure, reducing oxidative stress, enhancing adenosine triphosphate (ATP) production. Preclinical studies demonstrated protective restorative efficacy models heart failure, neurodegeneration, ischemia–reperfusion injury, syndromes, muscle atrophy weakness. Clinical trials PROGRESS-HF, TAZPOWER, MMPOWER-3, ReCLAIM elaborate on preclinical findings highlight significant therapeutic potential Elamipretide. Further research may expand its application to other diseases involving well investigate long-term safety drug. The following review synthesizes current knowledge mechanisms action, promising fitness, improving bioenergetics, minimizing stress.

Язык: Английский

Процитировано

4

A look at MERCs as UPRmt regulatory hubs in age-associated heart diseases DOI Open Access

Gabriela Navarrete-Anastasio,

Zeltzin Alejandra Ceja-Galicia, Cecilia Zazueta

и другие.

The Journal of Cardiovascular Aging, Год журнала: 2025, Номер 5(1)

Опубликована: Янв. 22, 2025

With the increase in life expectancy globally, challenge of dealing with aging becomes more prominent. Aging is a risk factor for several diseases, including cardiovascular disease. Mitochondria, which have long been studied relation to aging, play crucial role maintaining cellular homeostasis. However, there limitation interorganellar communication as organisms age. The unfolded protein response mitochondria (UPRmt) activated during stress maintain mitochondrial homeostasis and prevent accumulation damaged mitochondria. This involves signaling from nucleus, leading transcriptional changes. In context heart, this review explores terms function morphology. It also discusses impact UPRmt on cardiac diseases such heart failure, acute myocardial infarction, dilated cardiomyopathy. highlights potential mitochondria-endoplasmic reticulum contact sites (MERCs) modulating aging. Finally, it provides an update molecules that induce activity, potentially benefiting

Язык: Английский

Процитировано

0

Mitochondrial Electron Transport Chain Disruption and Oxidative Stress in Lipopolysaccharide-Induced Cardiac Dysfunction in rats and mice DOI
A. Sousa, Leonardo da Silva Chaves, Heberty Tarso Facundo

и другие.

Free Radical Research, Год журнала: 2025, Номер unknown, С. 1 - 14

Опубликована: Май 8, 2025

Sepsis, characterized by severe systemic inflammation and an excessive immune response to infection, is frequently triggered bacterial endotoxins like lipopolysaccharide (LPS) from Gram-negative bacteria. Moreover, sepsis-induced cardiac dysfunction remains a leading cause of mortality. This study aims elucidate the effects LPS-induced injury on mitochondrial damage, oxidative stress, subsequent dysfunction. LPS injections (in rats mice) for three days (1.5 mg/kg) impacted body weight increased TNF-α. Additionally, it decreased complexes I II activities while III IV remained unaffected. Disturbed in electron transport chain leads increase reactive oxygen species (ROS). Indeed, treatment significantly hydrogen peroxide production, reduced activity antioxidant enzymes catalase, superoxide dismutase, glutathione peroxidase, reductase activity. was accompanied cytosolic sulfhydryl proteins parallel cellular lipid peroxidation presence or absence Fe2+. LPS-treated samples had s-transferase activity, which may be attempt cell remove toxic products. In more acute Langendorff-perfused rat hearts, infusion (0.5 μg/mL) induced significant elevation left ventricular end-diastolic pressure decrease developed pressure. These findings harmful tissue could help development targeted therapies mitigate adverse

Язык: Английский

Процитировано

0

Aging and rejuvenation of engineered cardiovascular tissues: from research to clinical application DOI Open Access
Chang Liu,

Jing Guo,

Yanan Zhang

и другие.

The Journal of Cardiovascular Aging, Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Aging is a key contributor to the pathogenesis of cardiovascular diseases (CVDs). However, current methods and models CVD do not include factor aging due use premature cardiomyocytes. There an urgent need for engineered tissue (ECT) model that includes as greatest risk facilitate drug development aged patients. Cell therapy, which transplants pluripotent stem cell-derived cardiomyocytes in patients, was proved be effective cardiac repair, while cell retention rate limited. Alternatively, implantation ECT could enable long-term cells after translation may result rejuvenation hearts. This review summarizes features influencing factors tissues. The applications challenges myocardium designed clinical are also discussed.

Язык: Английский

Процитировано

0

High Dose Metformin Treatment to Inhibit Complex I during Early Reperfusion Protects the Aged Mouse Heart via Decreased MPTP Opening DOI
Qun Chen, Jeremy Thompson, Ying Hu

и другие.

Journal of Pharmacology and Experimental Therapeutics, Год журнала: 2024, Номер 392(3), С. 100529 - 100529

Опубликована: Дек. 24, 2024

Acute, high-dose metformin (MET, 2 mM) results in partial complex I inhibition ischemia (ISC)-modified mitochondria. Mitochondrial permeability transition pore (MPTP) opening increases cardiac injury during ISC-reperfusion (REP). We evaluated whether MET (2 can decrease MPTP aged hearts REP. Sestrin2 (Sesn2) regulates metabolism through activation of AMP-dependent protein kinase. Sesn2 decreases hearts. The knockout (KO) mimics the aging phenotype. Inactivation glycogen synthase kinase-3 β (GSK-3β) via serine-9 phosphorylation opening. assessed if mM given early REP by blockade with decreased and protection depends on Sesn2-mediated GSK-3β phosphorylation. C57BL/6BJ male mice (22-24 months) adult KO were evaluated. dose-dependently inhibited NADH oxidase activity permeabilized mitochondria both greater after 25 minutes ISC. infarct size improved calcium retention capacity wild-type mice. treatment only increased heart but not Thus, at partially inhibits susceptibility downstream is fully dependent inhibition. Complex downregulation acute, has translational potential to protect heart. SIGNIFICANCE STATEMENT: This study explores efficacy mechanism acute reducing mitochondrial-driven reperfusion stop-flow high-risk Metformin (NADH oxidation) ischemia-altered mitigated as injury. modulation application mitigate ST-elevation myocardial infarction

Язык: Английский

Процитировано

0