bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 13, 2023
Abstract
The
fast-growing
microbe
Vibrio
natriegens
is
capable
of
natural
transformation
where
it
draws
DNA
in
from
media
via
an
active
process
under
physiological
conditions.
Using
engineered
strain
with
a
genomic
copy
the
master
competence
regulator
tfoX
cholera
combination
new
minimal
(MCM)
that
uses
acetate
as
energy
source,
we
demonstrate
naturally
competent
cells
which
are
created,
transformed,
and
recovered
entirely
same
media,
without
exchange
or
addition
media.
Cells
to
plasmids,
recombination
linear
DNA,
co-transformation
both
select
for
scarless
markerless
edits.
entire
simple
inexpensive,
requiring
no
capital
equipment
room
temperature
(Zero
Capital
protocol,
10
4
cfu/
µ
g),
just
incubator
(High
Efficiency
5–6
g).
These
retain
their
state
when
frozen
transformable
immediately
upon
thawing
like
typical
chemical
electrochemical
cell.
Since
optimized
protocol
requires
only
50
minutes
hands-on
time,
V.
grows
quickly
even
on
plates,
started
at
9
AM
yields
abundant
culturable
single
colonies
by
5
PM.
Further,
because
all
stages
occur
can
be
arbitrarily
scaled
volume,
this
could
ideal
automated
directed
evolution
applications.
As
result,
compete
E.
coli
excellent
chassis
low-cost
highly
scalable
synthetic
biology.
Abstract
The
fast-growing
microbe
Vibrio
natriegens
is
capable
of
natural
transformation
where
it
draws
DNA
in
from
media
via
an
active
process
under
physiological
conditions.
Using
engineered
strain
with
a
genomic
copy
the
master
competence
regulator
tfoX
cholerae
combination
new
minimal
(MCM)
that
uses
acetate
as
energy
source,
we
demonstrate
naturally
competent
cells
which
are
created,
transformed,
and
recovered
entirely
same
media,
without
exchange
or
addition
fresh
media.
Cells
to
plasmids,
recombination
linear
DNA,
cotransformation
both
select
for
scarless
markerless
edits.
entire
simple
inexpensive,
requiring
no
capital
equipment
room
temperature
(zero
protocol,
104
cfu/μg),
just
incubator
(high-efficiency
105−6
cfu/μg).
These
retain
their
state
when
frozen
transformable
immediately
upon
thawing
like
typical
chemical
electrochemical
cell.
Since
optimized
protocol
requires
only
50
min
hands-on
time,
V.
grows
quickly
even
on
plates,
started
at
9
AM
yields
abundant
culturable
single
colonies
by
5
PM.
Further,
because
all
stages
occur
can
be
arbitrarily
scaled
volume,
this
could
ideal
automated
directed
evolution
applications.
As
result,
compete
Escherichia
coli
excellent
chassis
low-cost
highly
scalable
synthetic
biology.
Microbial Cell Factories,
Год журнала:
2023,
Номер
22(1)
Опубликована: Сен. 4, 2023
Pyruvate
is
a
widely
used
value-added
chemical
which
also
serves
as
hub
of
various
metabolic
pathways.
The
fastest-growing
bacterium
Vibrio
natriegens
promising
chassis
for
synthetic
biology
applications
with
high
substrate
uptake
rates.
aim
this
study
was
to
investigate
if
the
rates
V.
enable
pyruvate
production
at
productivities.Two
prophage
gene
clusters
and
several
essential
genes
biosynthesis
byproducts
were
first
deleted.
In
order
promote
accumulation,
key
aceE
encoding
dehydrogenase
complex
E1
component
down-regulated
reduce
carbon
flux
into
tricarboxylic
acid
cycle.
Afterwards,
expression
ppc
phosphoenolpyruvate
carboxylase
fine-tuned
balance
cell
growth
synthesis.
resulting
strain
PYR32
able
produce
54.22
g/L
from
glucose
within
16
h,
yield
1.17
mol/mol
an
average
productivity
3.39
g/L/h.
addition,
efficiently
convert
sucrose
or
gluconate
titers.A
novel
engineered
capable
provide
higher
in
This
lays
foundation
its
derivatives
fast-growing
natriegens.
Biotechnology Advances,
Год журнала:
2024,
Номер
77, С. 108464 - 108464
Опубликована: Окт. 9, 2024
Biotechnology
has
been
built
on
the
foundation
of
a
small
handful
well
characterized
and
well-engineered
organisms.
Recent
years
have
seen
breakout
performer
gain
attention
as
new
entrant
into
bioengineering
toolbox:
Vibrio
natriegens.
This
review
covers
recent
research
efforts
making
V.
natriegens
biotechnology
platform,
using
large
language
model
(LLM)
knowledge
graph
to
expedite
literature
survey
process.
Scientists
made
advancements
in
pertaining
fundamental
metabolic
characteristics
natriegens,
development
characterization
synthetic
biology
tools,
systems
analysis
modeling,
bioproduction
engineering,
microbial
ecology.
Each
these
subcategories
relevance
future
for
applications.
In
this
review,
we
cover
offer
context
impact
they
may
field,
highlighting
benefits
drawbacks
organism.
From
examining
research,
it
appears
that
is
precipice
becoming
platform
bacterium
biotechnology.
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 22, 2025
Vibrio
natriegens,
a
fast-growing
bacterium,
is
an
emerging
chassis
of
next-generation
industrial
biotechnology
capable
thriving
under
open
and
continuous
culture
conditions.
Cadaverine,
valuable
C5
platform
chemical,
has
various
chemical
biological
activities.
This
study
found
that
V.
natriegens
exhibited
superior
tolerance
to
lysine,
the
substrate
cadaverine
production.
For
first
time,
synthesis
pathway
was
introduced
into
for
whole-cell
catalysis
from
lysine.
A
high-efficiency
cadaverine-producing
strain
harboring
toxin–antitoxin
system,
(pSEVA341-pTac-ldcC-pHbpBC-hbpBC)
with
lysE
(PN96_RS17440)
inactivation,
constructed.
In
7
L
bioreactors,
titer
increased
115
g/L
in
original
158
within
11
h
biotransformation,
exhibiting
37%
increase
Its
productivity
reached
14.4
g/L/h
conversion
rate
as
high
90%.
These
results
confirm
exceptional
effective
bioproduction.
