Enhanced ROS Production and Mitochondrial Metabolic Shifts in CD4+ T Cells of an Autoimmune Uveitis Model DOI Open Access

Ronja Söth,

Anne Lisbeth Hoffmann,

Cornelia A. Deeg

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11513 - 11513

Опубликована: Окт. 26, 2024

Equine recurrent uveitis (ERU) is a spontaneously occurring autoimmune disease and one of the leading causes blindness in horses worldwide. Its similarities to autoimmune-mediated humans make it unique spontaneous animal model for this disease. Although many aspects ERU pathogenesis have been elucidated, remains not fully understood requires further research. CD4+ T cells particular focus In previous study, we showed metabolic alterations from cases, including an increased basal oxygen consumption rate (OCR) elevated compensatory glycolysis. To investigate underlying reasons consequences these changes, quantified reactive species (ROS) production cases compared healthy controls, revealing significantly higher ROS ERU-affected horses. Additionally, aimed define mitochondrial fuel oxidation glucose, glutamine, long-chain fatty acids (LCFAs) identified significant differences between controls. lower dependency on glucose greater flexibility LCFAs, indicating enhanced ability switch alternative fuels when necessary.

Язык: Английский

Unraveling the Role of Reactive Oxygen Species in T Lymphocyte Signaling DOI Open Access
Karsten Gülow,

Deniz Tümen,

Philipp Heumann

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 6114 - 6114

Опубликована: Июнь 1, 2024

Reactive oxygen species (ROS) are central to inter- and intracellular signaling. Their localized transient effects due their short half-life, especially when generated in controlled amounts. Upon T cell receptor (TCR) activation, regulated ROS signaling is primarily initiated by complexes I III of the electron transport chain (ETC). Subsequent production triggers activation nicotinamide adenine dinucleotide phosphate oxidase 2 (NADPH 2), prolonging oxidative signal. This signal then engages kinase cascades such as mitogen-activated protein (MAPK) pathway increases activity REDOX-sensitive transcription factors nuclear factor-kappa B (NF-κB) activator protein-1 (AP-1). To limit overproduction prevent stress, factor erythroid 2-related (Nrf2) antioxidant proteins superoxide dismutases (SODs) finely regulate intensity capable terminating needed. Thus, signals, well-controlled critical for cellular communication.

Язык: Английский

Процитировано

8

Immunometabolism of Liver Xenotransplantation and Prospective Solutions DOI Creative Commons
Shoulong Deng, Yi Zhang,

Shasha Shen

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

End-stage liver diseases, such as hepatocellular carcinoma or acute failure, critically necessitate transplantation. However, the shortage of available organ donors fails to meet rapidly growing transplantation demand. Due high similarity tissue structure and metabolism between miniature pigs humans, xenotransplantation pig livers is considered a potentially viable solution scarcity. In 2024, teams from China first time have successfully transplanted genetically modified Bama into clinically brain-dead man lasting for 10 days. This milestone in human research not only confirms feasibility clinical application xenotransplantation, but also underscores daunting protracted nature this pathway. Despite advanced gene-editing technologies theoretically circumventing occurrence most transplant rejection reactions, patients still face challenges chronic immune rejection, coagulation disorders, thrombotic microangiopathy after receiving xenografts. Moreover, prolonged use immunosuppressive drugs may induce irreversible dysfunction, leading opportunistic infections metabolic disorders. article compares similarities differences humans pigs, summarizes immunometabolism based on current findings, provides perspectives pre-transplantation post-transplantation strategies prolonging survival

Язык: Английский

Процитировано

0

Enhanced ROS Production and Mitochondrial Metabolic Shifts in CD4+ T Cells of an Autoimmune Uveitis Model DOI Open Access

Ronja Söth,

Anne Lisbeth Hoffmann,

Cornelia A. Deeg

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11513 - 11513

Опубликована: Окт. 26, 2024

Equine recurrent uveitis (ERU) is a spontaneously occurring autoimmune disease and one of the leading causes blindness in horses worldwide. Its similarities to autoimmune-mediated humans make it unique spontaneous animal model for this disease. Although many aspects ERU pathogenesis have been elucidated, remains not fully understood requires further research. CD4+ T cells particular focus In previous study, we showed metabolic alterations from cases, including an increased basal oxygen consumption rate (OCR) elevated compensatory glycolysis. To investigate underlying reasons consequences these changes, quantified reactive species (ROS) production cases compared healthy controls, revealing significantly higher ROS ERU-affected horses. Additionally, aimed define mitochondrial fuel oxidation glucose, glutamine, long-chain fatty acids (LCFAs) identified significant differences between controls. lower dependency on glucose greater flexibility LCFAs, indicating enhanced ability switch alternative fuels when necessary.

Язык: Английский

Процитировано

1